|Chapter 21 Genetic Basis of Development|
|1. Explain how advances in recombinant DNA technology have helped scientists study the eukaryotic genome.
2. Describe the natural function of restriction enzymes.
3. Describe how restriction enzymes and gel electrophoresis are used to isolate DNA fragments.
4. Explain how the creation of sticky ends by restriction enzymes is useful in producing a recombinant DNA molecule.
5. Outline the procedures for producing plasmid and phage vectors.
6. Explain how vectors are used in recombinant DNA technology.
7. List and describe the two major sources of genes for cloning.
8. Describe the function of reverse transcriptase in retroviruses and explain how they are useful in recombinant DNA technology.
9. Describe how “genes of interest” can be identified with the use of a probe.
10. Explain the importance of DNA synthesis and sequencing to modern studies of eukaryotic genomes.
11. Describe how bacteria can be induced to produce eukaryotic gene products.
12. List some advantages for using yeast in the production of gene products.
13. List and describe four complementary approaches used to map the human genome.
14. Explain how RFLP analysis and PCR can be applied to the Human Genome Project.
15. Describe how recombinant DNA technology can have medical applications such as diagnosis of genetic disease, development of gene therapy, vaccine production, and development of pharmaceutical products.
16. Describe how gene manipulation has practical applications for agriculture.
17. Describe how plant genes can be manipulated using the Ti plasmid carried by Agrobacterium as a vector.
18. Explain how foreign DNA may be transferred into monocotyledonous plants.
19. Describe how recombinant DNA studies and the biotechnology industry are regulated with regards to safety and policy matters.