Griffith’s Experiment
Griffith’s Experiment
Procedure (Internal Anatomy):
With scissors and beginning at the tip of the abdomen, make an incision (lengthwise) in the body covering slightly to the left of the mid-dorsal line and along the entire length of the grasshopper. Make a similar cut ventrally and also up the front of the head. Keep the inner scissors point just inside the body covering to avoid damaging the internal organs. If the specimen is a mature female, the interior spaces may be filled largely with slender eggs in the ovaries. Remove some of these is so directed by the instructor.
Locate the following organ systems:
1. Integument and exoskeleton
2. Muscular – In studying the other systems, note the many muscles, especially those connecting the wings and the legs.
3. Digestive system – remove some of the lateral muscles and trachea as necessary without injuring other organs. Identify the following structures in the digestive system.
a. *Esophagus
b. *Crop
c. *Gizzard
d. *Gastric caeca
e. *Stomach
f. *Intestine
g. *Rectum
h. *Anus
4. Circulatory system – heart
5. Respiratory system – tracheae
6. Excretory system – Malpighian tubules
7. Nervous system – brain, nerve cord
8. Reproductive system – testes, *ovaries, oviduct


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| Grasshopper Dissection | ![]() |
Introduction:
Insects are arthropods with jointed appendages, segmented bodies, and an exoskeleton composed of chitin. Insects are in the class Insecta, & are the largest and most diverse group of animals on earth. The genus Romalea is a large grasshopper common in the southeastern United States. Insects have three body regions (head, thorax, & abdomen), 3 pairs of legs attached to the thorax, a single pair of antenna attached to the head, mouthparts adapted for chewing or sucking, and two pairs of wings. Some insects may have a single pair of wings or be wingless. Insect legs are often adapted for digging, crawling, jumping, or swimming. The insects are mostly terrestrial, they breathe air which enters small lateral openings on the body called spiracles and circulates in a system of ducts to all organs and tissues. Their chewing or sucking mouth parts are adapted for feeding on plant or animal materials.
Classification:
Kingdom – Animalia
Phylum – Arthropoda
Class – Insecta
Order – Orthoptera
Objective:
Identify & label the internal & external anatomy of a grasshopper.
Materials:
Lab apron, gloves, eyeglasses, dissecting pan, dissecting kit with forceps & scalpel, t-pins, magnifying glass, preserved grasshopper, paper, pencil.
Procedure (External Anatomy): Examine the entire grasshopper and identify the major subdivisions and parts of the body.



| 1. Labrum | 4. Labium |
| 2. Mandibles | 5. Maxillary Palps |
| 3. Labial Palps | 6. Maxillae |
| 7. compound eye | 8. ocelli |
THORAX
Legs (first 2 pairs are for walking & the last pair are for jumping)
Wings (forewings have a leathery appearance & protect the hind wings)





ABDOMEN
Spiracles (small openings on the side of somites or body segments)
Auditory Organs (two located laterally on the 1st body somite or segment)
Ovipositor (on female)
Observations & Conclusion:
Figure 1 – Grasshopper Head (Label ALL parts.)
Figure 2 – External Grasshopper anatomy (Label ALL parts.)
Table 1 – External Appendages of the Grasshopper (Attach ALL parts.)
| Antenna
|
|
| Labrum
|
|
| Mandible
|
|
| Maxilla
|
|
| Labium
|
|
| Forewing
|
|
| Hindwing
|
|
| Walking Leg
|
|
| Jumping Leg
|
|
| Sex of Grasshopper |
1. Which region of the insect’s body is specialized for sensory functions? Explain your answer.
2. Which region of the insect’s body is specialized for movement & explain why?
3. What is the purpose of compound eyes? of simple eyes?
4. List the grasshopper’s mouthparts & their functions.
5. How are the ends of the legs adapted for holding onto plants?
6. How is the third pair of legs adapted for jumping?
7. Describe the differences between the two pairs of wings (appearance & function).
8. How does the tympanic membrane help a grasshopper?
9. What system do spiracles open into on a grasshopper?
10. Do all abdominal segments have spiracles? Are there any spiracles on the thoraic segments?
11. How did you determine the sex of your grasshopper?
12. Explain how grasshoppers dig holes to lay their eggs.
Graphing Practice

Introduction
Procedure 1:
1. What is the dependent variable and why? 2. What is the independent variable and why? 3. What title would you give the graph? . 4. What are the mean, median, and mode of all 3 columns of data? a). Depth : Mean____________Median__________Mode________ b). Bubble Plant A.: Mean ____________Median_________Mode________ c). Bubbles Plant B: Mean ____________Median_________Mode________ Graph Title: _________________________________________________________
Legend: ______________________________________________________________ Procedure 2: Answer the following questions concerning the data below and then graph it.
1. What is the dependent variable and why? 2. What is the independent variable and why? 3. What title would you give the graph? 4. Which, if any, of the above individuals (A or B) has diabetes? 5. What data do you have to support your hypothesis? 6. If the time period were extended to 6 hours, what would the expected blood glucose level for Person B? Title: ________________________________________________________________
Legend: ______________________________________________________________ Summary: 2. What conclusions can be determined from the data in graph 2? 3. Can the data in each of these graphs be used to construct other types of graphs? 4. If so, what other graph types can be constructed?
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