Chapter 3: Biochemistry PowerPoint Worksheet

Chapter 3 Biochemistry of Cells PowerPoint Notes

 

1. What is the most abundant organic compound on Earth?
2. Approximately how much water makes up the cells of organisms?
3. ___________ is known as the universal solvent.

 

4. List 4 properties of water that make it so useful to organisms.

 

 

5. Besides water, what other substance makes up most of the cell?

 

6. ____________ chemistry is the study of carbon compounds.

 

7. Carbon has _______ outer electrons so it can form ___________ bonds by sharing these electrons.

8. Carbon & hydrogen make up compounds called ________________.

 

9. Sketch a simple hydrocarbon with the formula CH4.

 

 

10. Carbon skeletons may be straight _______________, _____________ chains, or ______________ structures.

11. Hydrocarbons in ____________ supply our bodies with energy.

 

12. The _______ of an organic molecule determines its function.

13. ____________ groups give different properties to the organic compound to which they attach.

14. Write the formula for the following functional groups:

a. Hydroxyl

 

b. Carbonyl

 

c. Carboxyl

 

d. Amino

15. Give examples of organic compounds that contain each of the functional groups from question 14.

 

 

16. Large organic molecules are called _______________.

 

17. Polymers are built from smaller subunits called _____________.

 

18. Biologists call polymers _____________________.

 

19. Name 4 examples of polymers found in living things.

 

 

20. Monomers linked together are called ____________.

 

21. The process of linking monomers together is called _______________________.

 

22. Dehydration synthesis links small molecules or monomers together by removing molecules of _____________.

23. Name the process used to break down large polymers into smaller monomers.

 

24. Hydrolysis involves ____________ a molecule of water in order to break bonds.

 

25. Name some foods that contain lots of carbohydrates.

 

26. _________________ are simple sugars.

 

27. Name 3 monosaccharides & give their chemical formula.

 

 

28. Monosaccharides are called hexose sugars because they contain 6 _______________.

 

29. __________ is the simple sugar made by plants, ___________ is the sugar found in fruits, while _______________ is known as “milk sugar”.  Sugars have an __________ ending.

 

30. What are isomers?

 

31. Name 2 isomers.

32. What does aqueous mean?

33. What happens to simple sugars, monosaccharides, when they are put into aqueous solutions inside cells?

 

34. ___________________ serve as fuel for cells. Saccharide means ________________.

 

35. What is a double sugar called?

 

36. How are disaccharides formed? Name the BOND that joins them together.

 

37. Name 3 disaccharides.

 

38. Name the simple sugars that make up each of these disaccharides:

a. Sucrose

b. Maltose

c. Lactose

 

39. Complex carbohydrates are called ________________ & are made of chains of ________________________.

40. Name 3 examples of polysaccharides and tell the shape of each.

 

41. Plants store carbohydrate energy as ____________.

 

42. Name some starchy foods.

 

43. Animals store their carbohydrate energy as __________________.

 

44. Both starch & glycogen are made of monomers of ____________ or glucose.

 

45. Describe cellulose fibers & tell where in plants it is found.

 

 

46. Cellulose makes up __________ in plants and serves as dietary __________ in animals.

47. How are cows able to digest cellulose?

 

48. Since sugars dissolve in water, they are said to be _____________ or water-loving. What functional group makes them water soluble?

49. Lipids are hydrophobic. What does this mean?

 

50. Name 4 examples of lipids and then give 3 functions for lipids in the body.

Examples:

a.

b.

c.

 

51. If the bonds between carbons in a fatty acid are all single bonds, the fatty acid is ___________________.  Sketch a saturated fatty acid.

 

52. If there is a double bond between carbons in a fatty acid, the fatty acid is ___________________. Sketch an unsaturated fatty acid.

 

53. _______________ are the monomers that make up lipids or fats.

 

54. Triglycerides are made of an alcohol called ____________ and 3 ___________ acid chains.

55. ___________ forms the backbone of the fat. Sketch glycerol.

56. Saturated fatty acids are ___________ at room temperature and include __________,

margarine, and _____________.

57. Unsaturated fats in plants exist as ________ or oils at room temperature.

 

58. (a) What process links the 3 fatty acid chains to the glycerol in lipids?

(b) What lipids are in cell membranes?

(c) Sketch and label a phospholipid.

 

 

(d) Phospholipid heads are _____________ and attract water, while the 2 tails are _________ and repel water.

 

59. Lipids called _____________ are made of four, fused rings of carbon.

60. Name 3 steroids found in organisms.

a.

b.

c.

 

61. Proteins are polymers made of monomers called ___________________.

62. How many different amino acids are there?

 

63. Give 3 jobs for proteins in cells.

a.

b.

c.

 

64. What four things are bonded to the central carbon of every amino acid?

 

65. Sketch the structure of an amino acid & label the attached groups.

 

 

 

66. Amino acids are linked together by ____________ synthesis and held together by _____________ bonds.

67. Many proteins act as __________ or biological catalysts.

68. Cells have _____________ of enzymes which may ___________ chemical bonds and ____________ the amount of activation energy needed for the reaction to occur.

 

69. Enzymes have what shape?

 

70. Substrates attach to an enzyme at its ___________ site. When a substrate attaches to the active site the active site changes ________________.  This is called ______________ fit.

71. Can enzymes be reused?

 

72. The linear sequence of amino acids (chain) is the ____________ structure of a protein.

 

73. Protein chains are called __________________.

74. Secondary protein structures occur when proteins ___________ or ___________.

 

75. When polypeptides join together, the _________ groups interact with each other forming the ___________ structure of a protein forms.

76. Proteins take on a _____________ shape in the watery environment inside a cell. This is known as their _______________________ structure. Protein shape is also known as protein _____________________.

 

77. Denaturing a protein involves changing its __________ so it no longer works.

78. Name 2 things that denature proteins.

 

79. (a) What causes sickle cell anemia (disease)?

 

(b) What is the function of the protein hemoglobin in red blood cells?

 

(c) What protein controls blood sugar level?

(d) Insulin causes excess sugar to be stored in the _____________ as ________________.

(e) Proteins in the cell membrane that help cells recognize similar cells are called __________ proteins.

 

80. ___________ acids store hereditary information for making all of the body’s ______________.

 

81. Name the 2 types of nucleic acids.

 

82. What are the monomers for nucleic acids? Sketch a nucleotide.

 

83. Name the 4 bases on DNA.

84. What 2 things make up the sides of DNA?

 

85. DNA is ___________ stranded & coiled to make a shape called the double ____________.

 

86. RNA has __________ sugar instead of DEOXYRIBOSE sugar on DNA

 

87. RNA is a _____________ stranded molecule unlike double stranded DNA.

 

88. On RNA, the base ______________ replaces thymine.

89. _____________ is the cell’s energy molecule.

90. What is the monomer for ATP?

91. What does ATP stand for?

92. How is the nucleotide monomer for ATP DIFFERENT from the nucleotide monomer for nucleic acids?

93. Where is the energy stored in ATP?

94. Which bonds are considered HIGH ENERGY bonds in ATP?

95. When the last phosphate bond is broken, what is released?

96. what is the energy of ATP used for?

97. Besides energy, what two other things are formed when the last phospheta bond of ATP is broken?

98. How can ATP be reformed?

 

 

Chapter 11 – Cell Communication Objectives

 

 

Chapter 11   Cell Communication
Objectives
An Overview of Cell Signaling

1.  Describe the basic signal-transduction pathway used for mating in yeast. Explain why we believe these pathways evolved before the first multicellular organisms appeared on Earth.

2.  Define paracrine signaling and give an example.

3.  Define local regulation and explain why hormones are not local regulators.

4.  Explain how plant and animal hormones travel to target cells.

5.  List and briefly define the three stages of cell signaling.

Signal Reception and the Initiation of Transduction

6.  Describe the nature of a ligand-receptor interaction and state how such interactions initiate a signal-transduction system.

7.  State where signal receptors may be located in target cells.

8.  Compare and contrast G-protein-linked receptors, tyrosine-kinase receptors, and ligand-gated ion channels.

Signal-Transduction Pathways

9.  Describe two advantages of using a multistep pathway in the transduction stage of cell signaling.

10. Explain how the original signal molecule can produce a cellular response when it may not even enter the target cell.

11. Describe how phosphorylation propagates signal information.

12. Explain why a single cell may require hundreds of different protein kinases.

13. Explain how protein phosphatases turn off signal-transduction pathways.

14. Define the term second messenger. Briefly describe the role of these molecules in signaling pathways.

15. Describe how cyclic AMP is formed and how it propagates signal information in target cells.

16. Explain how the cholera bacterium causes the symptoms of cholera by disrupting G-protein-signaling pathways.

17. Describe how the cytosolic concentration of Ca2+ can be altered and how the increased pool of Ca2+ is involved with signal transduction.

Cellular Responses to Signals

18. Describe how signal information is transduced into cellular responses in the cytoplasm and in the nucleus.

19. Describe how signal amplification is accomplished in target cells.

20. Explain why different types of cells may respond differently to the same signal molecule.

21. Explain how scaffolding proteins help to coordinate a cell’s response to incoming signals.

 

BACK

 

Cell Respiration

 

Cellular Respiration
All Materials © Cmassengale

 

C6H12O6 + 6O2 —–> 6CO2 + 6H20 + energy (heat and ATP)

Energy

  • Capacity to move or change matter
  • Forms of energy are important to life include Chemical, radiant (heat & light), mechanical, and electrical
  • Energy can be transformed from one form to another
  • Chemical energy is the energy contained in the chemical bonds of molecules
  • Radiant energy travels in waves and is sometimes called electromagnetic energy. An example is visible light
  • Photosynthesis converts light energy to chemical energy
  • Energy that is stored is called potential energy

Laws of Thermodynamics

  • 1st law- Energy cannot be created or destroyed.

    Energy can be converted from one form to another. The sum of the energy before the conversion is equal to the sum of the energy after the conversion.

  • 2nd law- Some usable energy is lost during transformations.

    During changes from one form of energy to another, some usable energy is lost, usually as heat. The amount of usable energy therefore decreases.

 

Adenosine triphosphate (ATP)

  • Energy carrying molecule used by cells to fuel their cellular processes
  • ATP is composed of an adenine base, ribose sugar, & 3 phosphate (PO4) groups

 

 

 

  • The PO4 bonds are high-energy bonds that require energy to be made & release energy when broken

 

 

  • ATP is made & used continuously by cells
  • Every minute all of an organism’s ATP is recycled
  • Phosphorylation refers to the chemical reactions that make ATP by adding Pi to ADP ADP + Pi + energy «  ATP + H2O
  • Enzymes  (ATP synthetase& ATPase) help break & reform these high energy PO4 bonds in a process called substrate-level phosphorylation
  • When the high-energy phosphate bond is broken, it releases energy, a free phosphate group, & adenosine diphosphate (ADP)

 

 

 

Enzymes in Metabolic Pathways:

  • Biological catalysts
  • Speeds up chemical reactions
  • Lowers the amount of activation energy needed by weakening existing bonds in substrates

  • Highly specific protein molecules
  • Have an area called the active site where substrates temporarily join
  • Form an enzyme-substrate complex to stress bonds
  • Enzyme usable

enzyme substrate complex

 
Energy Carriers During Respiration:

NADH: A second energy carrying molecule in the mitochondria; produces 3 ATP

 

 

FADH2: A third energy carrying molecule in the mitochondria; produces 2 ATP

 

 

Mitochondria:

  • Has outer smooth, outer membrane & folded inner membrane
  • Folds are called cristae
  • Space inside cristae is called the matrix & contains DNA & ribosomes
  • Site of aerobic respiration
  • Krebs cycle takes place in matrix
  • Electron Transport Chain takes place in cristae 

Cellular Respiration Overview:

C6H12O6 + 6O2 —–> 6CO2 + 6H20 + energy (heat and ATP)

  • Controlled release of energy from organic molecules (most often glucose)
  • Glucose is oxidized (loses e-) & oxygen is reduced (gains e-)
  • The carbon atoms of glucose (C6H12O6) are released as CO2
  • Generates ATP (adenosine triphosphate)

 

 

  • The energy in one glucose molecule may be used to produce 36 ATP
  • Involves a series of 3 reactions — Glycolysis, Kreb’s Cycle, & Electron Transport Chain

Glycolysis:

  • Occurs in the cytoplasm
  • Summary of the steps of Glycolysis:

    a. 2 ATP added to glucose (6C) to energize it.

    b. Glucose split to 2 PGAL (3C). (PGAL = phosphoglyceraldehyde)

    c. H+ and e- (e- = electron) taken from each PGAL & given to make 2 NADH.

    d. NADH is energy and e- carrier.

    e. Each PGAL rearranged into pyruvate (3C), with energy transferred to make 4 ATP (substrate phosphorylation).

    f. Although glycolysis makes 4 ATP, the net ATP production by this step is 2 ATP (because 2 ATP were used to start glycolysis). The 2 net ATP are available for cell use.

    g. If oxygen is available to the cell, the pyruvate will move into the mitochondria & aerobic respiration will begin.


     

    Net Yield from Glycolysis
    4 NADH2
    2 CO2
    4 ATP ( 2 used to start reaction)

     

h. If no oxygen is available to the cell (anaerobic), the pyruvate will be fermented by addition of 2 H from the NADH (to alcohol + CO2 in yeast or lactic acid in muscle cells). This changes NADH back to NAD+ so it is available for step c above. This keeps glycolysis going!

 

Alcoholic Fermentation

 

 

Lactic Acid Fermentation

 

Aerobic Respiration:

  • Occurs in the mitochondria
  • Includes the Krebs Cycle & the Electron Transport Chain
  • Pyruvic acid from glycolysis diffuses into matrix of mitochondria & reacts with coenzyme A to for acetyl-CoA (2-carbon compound)
  • CO2 and NADH are also produced

Kreb’s Cycle:

  • Named for biochemist Hans Krebs
  • Metabolic pathway that indirectly requires O2 
  • Kreb’s Cycle is also known as the Citric acid Cycle
  • Requires 2 cycles to metabolize glucose
  • Acetyl Co-A (2C) enters the Kreb’s Cycle & joins with Oxaloacetic Acid (4C) to make Citric Acid (6C)
  • Citric acid is oxidized releasing CO2 , free H+, & e- and forming ketoglutaric acid (5C)
  • Free e- reduce the energy carriers NAD+ to NADH2 and FAD+ to FADH2
  • Ketoglutaric acid is also oxidized releasing more CO2 , free H+, & e-
  • The cycle continues oxidizing the carbon compounds formed (succinic acid, fumaric acid, malic acid, etc.) producing more CO2, NADH2, FADH2, & ATP
  • H2O is added to supply more H+
  • CO2 is a waste product that diffuses out of cells
  • Oxaloacetic acid is regenerated to start the cycle again
  • NADH2 and FADH2 produced migrate to the Electron Transport Chain (ETC)

 

Net Yield from Kreb’s Cycle (2 turns)
6 NADH2
2 FADH2
4 CO2
2 ATP

 

Electron Transport Chain:

  • Found in the inner mitochondrial membrane or cristae
  • Contains 4 protein-based complexes that work in sequence moving H+ from the matrix across the inner membrane (proton pumps)
  • A concentration gradient of H+ between the inner & outer mitochondrial membrane occurs
  • H+ concentration gradient causes the synthesis of ATP by chemiosmosis
  • Energized e- & H+ from the 10 NADH2 and 2 FADH2 (produced during glycolysis & Krebs cycle) are transferred to O2 to produce H2O (redox reaction)

O2  +  4e-  +  4H+  2H2O

 

Energy Yield from Aerobic Respiration
Glycolysis Kreb’s Cycle Total
4 NADH2 6 NADH2 10 NADH2 x 3 = 30 ATP
0 FADH2 2 FADH2 2 FADH2 x 2 = 4 ATP
2 ATP 2 ATP                          4 ATP
38 ATP

 

  • Most cells produce 36- 38 molecules of ATP per glucose (66% efficient)
  • Actual number of ATP’s produced by aerobic respiration varies among cells

 

Cell Reproduction Lecture Notes B1

Cell Reproduction Lecture Guide

SECTION 8-1    CHROMOSOMES

DNA stores?                        Estimated length?

Coiled DNA in eukaryote nucleus called?

Chromosome shape?                              Made of?

Can be seen inside nucleus by?

Histones?

Function of histones?

Function of nonhistone proteins?

Sister chromatids?

When form?

Centromere?

Function of centromere?

Sketch sister chromatids & label centromere.

Prokaryotic chromosomes?

Shape?                         Number?                     Location & attachment?

Number of chromosomes in human body cells?               Called what?

How abbreviated?

Are all diploid numbers in organisms the same?   Explain and give examples.

Human body cells called what?               Examples?

Reproductive cells are called?                        Name them.

Chromosome number of gametes?                              Abbreviation?

Haploid number also called?                                      Haploid number for humans?

Fertilization?

Chromosome number that fertilization restores?

Fertilized egg called?                          Sets of chromosomes in zygote?

Chromosomes in egg and sperm called?             Name them.

Sex chromosomes of female?                                 Male?

Other 22 pairs or 44  chromosomes called?

Karyotype?

Homologous pairs of chromosomes?

Example of information contained in homologs.

SECTION 8-2    CELL DIVISION

All cells come from?                          Process called?                     

Same in prokaryotes & eukaryotes?

Binary fission?

Used by?                               Number of steps or stages.

Stage 1 of binary fission?

Stage 2 of binary fisssion?

Stage 3 of binary fission?

Is binary fission sexual or asexual reproduction?

Original cell that forms eukaryotes is called?

How new cells compare to each other & the original cell after cell division? Why?

Phases cell goes through in its life cycle called?

Number of phases?                  Name them.

Two parts of cell division?

Mitosis?

Interphase?

Also called?                          Length in cell cycle?

What’s occurring to cells in interphase?

Number of phases in interphase?                   Name them.

G1 phase?

S phase?

G2 phase?

Replication?

Results in forming?                                            Occurs when?

Why all new cells must have exact copy of DNA?

Daughter cells?

How form?                                       Compare to each other?

Two steps of cell division called?

Another name for mitosis?                                    What’s dividing?

Division of the cytoplasm called?                          When occurs?

Parent cell?

How multicellular organisms grow?

Number of steps or phases in mitosis?            Name them in order.

What’s made during mitosis?

When did the chromosomes replicate (make copies of the DNA)?

Prophase?

Chromatin condenses into what?                                 Held together by?

Two structures that disappear in prophase?

Centrosomes located near?                                        Number of centrosomes?

Contain what cylindrical bodies?                                 Found in plant &/or animal cells?
Made of bundles of?                                                   Where centrosomes move?

Help form?

Function of mitotic spindle?

Two types of spindle fibers?

Attach to centromere of sister chromatids?                                          Function?

Metaphase?

             Where are chromosomes moved?

What moves the chromosomes?

Center of cell called?                                        Ends of cell called?

Anaphase?

What happens to sister chromatids?

Once chromatids separate, they’re now individual what?

Telophase?

What happens to spindle fibers?

Chromosomes again tightly coil becoming what?

What two structures reform?

Cytokinesis?

How occurs in animal cells?

How occurs in plant cells?

How many new cells formed?                                    Cells called?

Size of new cells to each other?                   Size of new cells & parent cell?

Daughter cells & parent cell genetically identical or different?

Is mitosis sexual or asexual reproduction?

SECTION 8-3    MEIOSIS

Meiosis?

What happens to chromosome number?

Cells produced by meiosis are called?              Their chromosome number?

Fusion of gametes?                                                      Effect on chromosome number?

Number of chromosomes in human egg?               Sperm?            Zygote?

Sexual reproduction?

Combines what 2 cells?                                                Forms what cell?

Eggs?

Sperm?

How sperm reaches nonmotile egg?

Gametes produced by what process?

Where occurs in females?                                            In males?

What called in females?                                               In males?

Diploid egg or sperm after meiosis have what chromosome number?

How do daughter cells made in meiosis compare to the original cell?

How many divisions do cells undergo during meiosis?

How many new cells are produced?

How many main stages are there in meiosis?                                   Name them.

What occurs in Meiosis I?

What occurs in Meiosis II?

Chromosome number at the beginning of Meiosis I?

Homolog?

Synapsis?

Pair of homologs after synapsis called?                                    Sketch a tetrad.

First step in Meiosis I called?

            Are chromosomes visible?

Chromosome number in meiosis I?

Genes?

Crossing over?

Genetic recombination?

What 2 structures disappear?

What structure appears & attaches to homologs?

Where are tetrads moved during Metaphase I?

What happens to homologs in Anaphase I?

            What separates the homologs?

Random separation of homologous chromosomes called?

What happens to cytoplasm during Telophase I?

Chromosome number of new cells?                             How many new cells formed?

Do chromosomes replicate before Meiosis II?

            Name the 4 steps in Meiosis II.

How many new cells form in males?                          In females?

Polar bodies?

What usually happens to polar bodies? Why?

New cells in females called?                                      Cells after maturing called?

New cells in males called?                                         Cells after maturing called?

Chromosome number of new cells?

Evolution?

Which type of reproduction causes change in organisms?

Reproduction involving one parent?                                               Give 3 examples.

Chromosome number of parent & new cells?

Reproduction involving two parents?

Chromosome number of parent cell?                           Chromosome number of new cells?

Are organisms in a sexually reproducing population genetically identical?

Variations?

“Survival of the fittest”?

How environmental changes affect asexually reproducing organisms?        Sexually reproducing organisms?

BACK

Chapter 2 – Chemistry Outline

 

Student Name
Date
Period
Chapter 2        Chemistry Outline
I. Composition of Matter
         A. Matter
  * 1.1  1. Makes up everything
             2. Occupies space & has mass
             3. Mass – quantity of matter making up an object
             4. Pull of gravity produces weight
             5. Chemical changes in matter necessary for life processes
   B. Elements and Atoms
           1. Pure substances that can’t be chemically broken down
           2. about 30 elements important to organisms
           3. Most common elements are C, H, O, N
           4. Symbols (1-2 letters) stand for elements
             5. May use Latin or Greek  e.g. Na – sodium (natrium)
*1.2 6. Simplest part of an element with the same properties
                                
 C. Nucleus
           1.  Most of the mass
           2. Central part of an atom
           3.