Structure & Function of the Cells

 

STRUCTURE AND FUNCTION OF THE CELL

All Materials © Cmassengale

I. All Organisms are Made of Cells

 

A. The cell is the basic unit of structure & function

 

B. The cell is the smallest unit that can still carry on all life processes

C. Both unicellular (one celled) and multicellular (many celled) organisms are composed of cells

D. Before the 17th century, no one knew cells existed

E. Most cells are too small to be seen with the unaided eye

 

F. In the early 17th century microscopes were invented & cells were seen for the 1st time

G. Anton Von Leeuwenhoek, a Dutchman, made the 1st hand-held microscope & viewed microscopic organisms in water & bacteria from his teeth

 

Leeuwenhoek’s microscope consisted simply of:

  • A) a screw for adjusting the height of the object being examined
  • B) a metal plate serving as the body
  • C) a skewer to impale the object and rotate it
  • D) the lens itself, which was spherical

 

 

H. In 1665, an English scientist named Robert Hooke made an improved microscope and viewed thin slices of cork viewing plant cell walls

 

I. Hooke named what he saw “cells”

J. In the 1830’s, Matthias Schleiden (botanist studying plants) & Theodore Schwann (zoologist studying animals) stated that all living things were made of cells

 

K. In 1855, Rudolf Virchow stated that cells only arise from pre-existing cells

 

L. Virchow’s idea contradicted the idea of spontaneous generation (idea that nonliving things could give rise to organisms)

M. The combined work of Schleiden, Schwann, & Virchow is known as the Cell Theory

 

Schwann Schleiden Virchow

 

 

II. Principles of the Cell Theory

 

A. All living things are made of one or more cells

B. Cells are the basic unit of structure & function in organisms

C. Cells come only from the reproduction of existing cells

 

III. Cell Diversity

 

A. Not all cells are alike

B. Cells differ in size, shape, and function

 

C. The female egg cell is the largest cell in the body & can be seen without a microscope

relative sizes of cells and their components

D. Bacterial cells are some of the smallest cells & are only visible with a microscope


E.coli Bacterial Cells

E. Cells need surface area of their cell membrane large enough to adequately exchange materials with the environment (wastes, gases such as O2 & CO2, and nutrients)

 

F. Cells are limited in size by the ratio between their outer surface area & their volume

 

G. Small cells have more surface area for their volume of cytoplasm than large cells

H. As cells grow, the amount of surface area becomes too small to allow materials to enter & leave the cell quickly enough

I. Cell size is also limited by the amount of cytoplasmic activity that the cell’s nucleus can control

J. Cells come in a variety of shapes, & the shape helps determine the function of the cell (e.g. Nerve cells are long to transmit messages in the body, while red blood cells are disk shaped to move through blood vessels)

 

IV. Prokaryotes

 

A. Prokaryotic cells are less complex

B. Unicellular

C. Do not have a nucleus & no membrane-bound organelles

 

D. Most have a cell wall surrounding the cell membrane & a single, looped chromosome (genetic material) in the cytoplasm

 

E. Include bacteria & blue-green bacteria

 

F. Found in the kingdom Monera

 

 

V. Eukaryotes

 

A. More complex cells

B. Includes both unicellular & multicellular organisms

 

C. Do have a true nucleus & membrane-bound organelles

 

D. Organelles are internal structures in cell’s that perform specific functions

 

a. Nucleus b. Chloroplast c. Golgi d. Mitochondria

 

E. Organelles are surrounded by a single or double membrane

 

F. Entire eukaryotic cell surrounded by a thin cell membrane that controls what enters & leaves the cell

G. Nucleus is located in the center of the cell

H. The nucleus contains the genetic material (DNA) & controls the cell’s activities

I. Eukaryotes include plant cells, animal cells, fungi, algae, & protists

J. Prokaryotes or bacteria lack a nucleus

K. Found in the kingdoms Protista, Fungi, Plantae, & Animalia

 

 

VI. Cell Membrane

 

A. Separates the cytoplasm of the cell from its environment

B. Protects the cell & controls what enters and leaves

 

C. Cell membranes are selectively permeable only allowing certain materials to enter or leave

D. Composed of a lipid bilayer made of phospholipid molecules

 

E. The hydrophilic head of a phospholipid is polar & composed of a glycerol & phosphate group and points to the aqueous cytoplasm and external environment.

F. The two hydrophobic tails are nonpolar point toward each other in the center of the membrane & are composed of two fatty acids

G. When phospholipids are placed in water, they line up on the water’s surface with their heads sticking into the water & their tails pointing upward from the surface.

H. The inside of the cell or cytoplasm is an aqueous or watery environment & so is the outside of the cell. Phospholipid “heads” point toward the water.

I. Phospholipid “tails” are sandwiched inside the lipid bilayer.

J. The cell membrane is constantly breaking down & being reformed inside living cells.

K. Certain small molecules such as CO2, H2O, & O2 can easily pass through the phospholipids

 

VII. Membrane Proteins

 

A. A variety of protein molecules are embedded in the cell’s lipid bilayer.

B. Some proteins called peripheral proteins are attached to the external & internal surface of the cell membrane

C. Integral proteins or transmembrane proteins are embedded & extend across the entire cell membrane. These are exposed to both the inside of the cell & the exterior environment.

D. Other integral proteins extend only to the inside or only to the exterior surface.

E. Cell membrane proteins help move materials into & out of the cell.

F. Some integral proteins called channel proteins have holes or pores through them so certain substances can cross the cell membrane.

G. Channel proteins help move ions (charged particles) such as Na+, Ca+, & K+ across the cell membrane

H. Transmembrane proteins bind to a substance on one side of the membrane & carry it to the other side. e.g. glucose

 

I. Some embedded, integral proteins have carbohydrate chains attached to them to serve as chemical signals to help cells recognize each other or for hormones or viruses to attach

 

 

VIII. Fluid Mosaic Model

 

A. The phospholipids & proteins in a cell membrane can drift or move side to side making the membrane appear “fluid”.

B. The proteins embedded in the cell membrane form patterns or mosaics.

C. Because the membrane is fluid with a pattern or mosaic of proteins, the modern view of the cell membrane is called the fluid mosaic model.

 

IX. Internal Cell Structure & Organelles of Eukaryotes

A. Cytoplasm includes everything between the nucleus and cell membrane.

 

B. Cytoplasm is composed of organelles & cytosol (jellylike material consisting of mainly water along with proteins.

 

C. Eukaryotes have membrane-bound organelles; prokaryotes do not

 

D. Mitochondria are large organelles with double membranes where cellular respiration (breaking down glucose to get energy) occurs

1. Energy from glucose is used to make ATP or adenosine triphosphate

 

2. Cells use the ATP molecule for energy

3. More active cells like muscle cells have more mitochondria

 

4. Outer membrane is smooth, while inner membrane has long folds called cristae

 

5. Have their own DNA to make more mitochondria when needed

E. Ribosomes are not surrounded by a membrane & are where proteins are made in the cytoplasm (protein synthesis)

 

1. Most numerous organelle

2. May be free in the cytoplasm or attached to the rough ER (endoplasmic reticulum)

F. Endoplasmic reticulum are membranous tubules & sacs that transport molecules from one part of the cell to another

1. Rough ER has embedded ribosomes on its surfaces for making proteins

2. Smooth ER lacks ribosomes & helps break down poisons, wastes, & other toxic chemicals

3. Smooth ER also helps process carbohydrates & lipids (fats)

4. The ER network connects the nucleus with the cell membrane

 

G. Golgi Apparatus modifies, packages, & helps secrete cell products such as proteins and hormones

1. Consists of a stack of flattened sacs called cisternae

 

2. Receives products made by the ER

 

H. Lysosomes are small organelles containing hydrolytic enzymes to digest materials for the cell

1. Single membrane

2. Formed from the ends of Golgi that pinch off

 

3. Found in most cells except plant cells

I. Cytoskeleton consists of a network of long protein tubes & strands in the cytoplasm to give cells shape and helps move organelles

 

1. Composed of 2 protein structures — microtubules, intermediate filaments, & microfilaments

 

2. Microfilaments are ropelike structures made of 2 twisted strands of the protein actin capable of contracting to cause cellular movement (muscle cells have many microfilaments)

3. Microtubules are larger, hollow tubules of the protein called tubulin that maintain cell shape, serve as tracks for organelle movement, & help cells divide by forming spindle fibers that separate chromosome pairs

 

Cytoskeleton Element  General Function
Microtubules Move materials within the cell
Move the cilia and flagella
Actin Filaments Move the cell
Intermediate Filaments Provides mechanical support

 

 

J. Cilia are short, more numerous hair like structures made of bundles of microtubules to help cells move

 

1. Line respiratory tract to remove dust & move paramecia


Cross section of Cilia & Flagella

K. Flagella are long whip like tails of microtubules bundles used for movement (usually 1-3 in number)

1. Help sperm cells swim to egg

L. Nucleus (nuclei) in the middle of the cell contains DNA (hereditary material of the cell) & acts as the control center

 

1. Most cells have 1 nucleolus, but some have several

2. Has a protein skeleton to keep its shape

3. Surrounded by a double layer called the nuclear envelope containing pores

4. Chromatin is the long strand of DNA in the nucleus, which coils during cell division to make chromosomes

 

5. Nucleolus (nucleoli) inside the nucleus makes ribosomes & disappears during cell division

 

M. Cell walls are nonliving, protective layers around the cell membrane in plants, bacteria, & fungi

1. Fungal cell walls are made of chitin, while plant cell walls are made of cellulose

 

2. Consist of a primary cell wall made first and a woody secondary cell wall in some plants

 

N. Vacuoles are the largest organelle in plants taking up most of the space

1. Serves as a storage area for proteins, ions, wastes, and cell products such as glucose

 

2. May contain poisons to keep animals from eating them

3. Animal vacuoles are smaller & used for digestion

O. Plastids in plants make or store food & contain pigments to trap sunlight

1. Chloroplast is a plastid that captures sunlight to make O2 and glucose during photosynthesis; contains chlorophyll

a. Double membrane organelle with an inner system of membranous sacs called thylakoids

 

b. Thylakoids made of stacks of grana containing chlorophyll

2. Other plastids contain red, orange, and yellow pigments

3. Found in plants, algae, & seaweed

X. Multicellular Organization

 

A. Cells are specialized to perform one or a few functions in multicellular organisms

B. Cells in multicellular organisms depend on each other

 

C. The levels of organization include:
Cells –> Tissues –> Organs –> Systems –> Organism

D. Tissues are groups of cells that performs a particular function (e.g. Muscle)

 

E. Organs are groups of tissues working together to do a job (e.g. heart, lungs, kidneys, brain)

F. Systems are made of several organs working together to carry out a life process (e.g. Respiratory system for breathing)

G. Plants have specialized tissues & organs different from animals

1. Dermal tissue forms the outer covering of plants

2. Ground tissue makes up roots & stems

3. Vascular tissue transports food & water

4. The four plant organs are the root, stem, leaf, & flower

 

H. Colonial organisms are made of cells living closely together in a connected group but without tissues & organs (e.g. Volvox)

Cell Cycle & Division

 

 

Cell Cycle & Division
All Materials © Cmassengale

 

Cell Division:

  • All cells are derived from preexisting cells (Cell Theory)
  • Cell division is the process by which cells produce new cells
  • Cell division differs in prokaryotes (bacteria) and eukaryotes (protists, fungi, plants, & animals)
  • Some tissues must be repaired often such as the lining of gut, white blood cells, skin cells with a short lifespan 
  • Other cells do not divide at all after birth such as muscle & nerve 

Reasons for Cell Division:

  • Cell growth
  • Repair & replacement of damaged cell parts
  • Reproduction of the species

Copying DNA: 

  • Since the instructions for making cell parts are encoded in the DNA, each new cell must get a complete set of the DNA molecules
  • This requires that the DNA be copied (replicated, duplicated) before cell division

Replication process

Chromosomes & Their Structure:

  • The plans for making cells are coded in DNA
  • DNA, deoxyribose nucleic acid, is a long thin molecule that stores genetic information
  • DNA in a human cell is estimated to consist of six billion pairs of nucleotides
  • DNA is organized into giant molecules called chromosomes
  • Chromosomes are made of protein & a long, single, tightly-coiled DNA molecule visible only when the cell divides
  • When a cell is not dividing the DNA is less visible & is called chromatin
  • DNA in eukaryotic cells wraps tightly around proteins called histones to help pack the DNA during cell division
  • Nonhistone proteins help control the activity of specific DNA genes
  • Kinetochore proteins bind to centromere and attach chromosome to the spindle in mitosis
  • Centromeres hold duplicated chromosomes together before they are separated in mitosis
  • Telomeres are the ends of chromosomes which are important in cell aging
  • When DNA makes copies of itself before cell division, each half of the chromosome is called a sister chromatid

  • DNA of prokaryotes (bacteria) is one, circular chromosome attached to the inside of the cell membrane

Chromosome Numbers:

  • Humans somatic or body cells have 23 pairs of chromosomes or 46 chromosomes (diploid or 2n number)
  • The 2 chromatids of a chromosome pair are called homologues (have genes for the same trait at the same location)


Homologs

  • Human reproductive cells or gametes (sperms & eggs) have one set or 23 chromosomes (haploid or n number)
  • Every organism has a specific chromosome number

 

Organism Chromosome Number (2n)
Human 46
Fruit fly 8
Lettuce 14
Goldfish 94

 

  • Fertilization, joining of the egg & sperm, restores the diploid chromosome number in the zygote (fertilized egg cell)
  • Sex chromosomes, either X or Y, determine the sex of the organism
  • Two X chromosomes, XX, will be female and XY will be male
  • All other chromosomes, except X & Y, are called autosomes
  • Chromosomes from a cell may be arranged in pairs by size starting with the longest pair and ending with the sex chromosomes to make a karyotype
  • A human karyotype has 22 pairs of autosomes and 1 pair of sex chromosomes (23 total)


Human Male Karyotype

Genes:

  • A section of DNA which codes for a protein is called a gene
  • Each gene codes for one protein
  • Humans have approximately 50,000 genes or 2000 per chromosome
  • About 95% of the DNA in chromosome is “junk” that does not code for any proteins

Cell Cycle:

  • Cells go through phases or a cell cycle during their life before they divide to form new cells 
  • The cell cycle includes 2 main parts — interphase, and cell division
  • Cell division includes mitosis (nuclear division) and cytokinesis (division of the cytoplasm)
  • Interphase is the longest part of a cell’s life cycle and is called the “resting stage” because the cell isn’t dividing
  • Cells grow, develop, & carry on all their normal metabolic functions during interphase
  • Interphase consists of 3 parts — G1, S, & G2phases

Interphase:

  • G1 or 1st Growth Phase occurs after a cell has undergone cell division
  • Cells mature & increase in size by making more cytoplasm & organelles while carrying normal metabolic activities in G1 
  • S or Synthesis Phase follows  G1  and the genetic material of the cell (DNA) is copied or replicated 

  • G2 or 2nd Growth Phase occurs after S Phase and the cell makes all the structures needed to divide

Cell division in Prokaryotes:

  • Prokaryotes such as bacteria do not have a nucleus
  • Prokaryotes divide into two identical new cells by the process of binary fission
  • Binary fission is an asexual method of reproduction
  • In binary fission,  the chromosome, attached to cell membrane, makes a copy of itself and the cell grows to about twice its normal size
  • Next, a cell wall forms between the chromosomes & the parent cell splits into 2 new identical daughter cells (clones)


Cell Division in Eukaryotes:

  • Eukaryotes have a nucleus & membrane-bound organelles which must be copied exactly so the 2 new cells formed from division will be exactly alike
  • The original parent cell & 2 new daughter cells must have identical chromosomes
  • DNA is copied in the S phase of the cell cycle & organelles, found in the cytoplasm,  are copied in the Growth phases
  • Both the nucleus (mitosis) and the cytoplasm (cytokinesis) must be divided during cell division in eukaryotes

Stages of Mitosis:

  • Division of the nucleus or mitosis occurs first
  • Mitosis is an asexual method of reproduction
  • Mitosis consists of 4 stages — Prophase, Metaphase, anaphase, & Telophase

  • Prophase:
    • Chromosomes become visible when they condense into sister chromatids
    • Sister chromatids attach to each other by the centromere
    • Centrioles in animal cells move to opposite ends of cell
    • Spindle forms from centriole (animals) or microtubules (plants)
    • Kinetochore fibers of spindle attach to centromere
    • Polar fibers of spindle extend across cell from pole to pole
    • Nuclear membrane dissolves
    • Nucleolus disintegrates
  • Metaphase:
    • Chromosomes line up in center or equator of the cell attached to kinetochore fibers of the spindle
  • Anaphase:
    • Kinetochore fibers attached to the centromere pull the sister chromatids apart
    • Chromosomes move toward opposite ends of cell
  • Telophase:
    • Nuclear membrane forms at each end of the cell around the chromosomes
    • Nucleolus reform
    • Chromosomes become less tightly coiled & appear as chromatin again
    • Cytokinesis begins

Cytokinesis:

  • Cytoplasm of the cell and its organelles separate into 2 new daughter cells
  • In animals, a groove called the cleavage furrow forms pinching the parent cell in two

  • In plants, a cell plate forms down the middle of the cell where the new cell wall will be

Summary of Mitosis:

 

 
 
 Interphase

 

  1. Cell matures & carries on normal activities
  2. DNA copied & appears as chromatin
  3. Nucleolus visible
 Early Prophase  

 

  1. Chromosomes condense & become visible
  2. Centrioles separate & spindle starts forming
 
 Late Prophase

  1. Spindle forms with aster at each pole
  2. Nuclear membrane & nucleolus disintegrate
  3. Centromere of chromosomes attaches to spindle fibers
 Metaphase

  1. Chromosomes line up at the equator of the cell attached to kinetochore fibers of spindle
 Anaphase

  1. Centromeres split apart
  2. Homologs move to opposite poles of the cell
 Telophase/Cytokinesis  

  1. Nuclear membrane & nucleolus reform
  2. Cell pinches into 2 cells in animals
  3. In plants, a cell plate separates the 2 new cells

 

Cancer is Uncontrolled Mitosis:

  • Mitosis must be controlled, otherwise growth will occur without limit (cancer)
  • Control is by special proteins produced by oncogenes
  • Mutations in control proteins can cause cancer

Meiosis & Sexual Reproduction

  • Reduces the number of chromosomes in new cells to half the number in the original cell
  • New cells have a single copy of chromosomes (23 total) but are not identical to each other or the original parent cell
  • Used for making gametes ( sperm and eggs) with the haploid or n number
  • In meiosis, cells divide twice after a single DNA duplication
  • Meiosis I separates homologs & the Meiosis II separates sister chromatids
  • Meiosis I stages are Prophase I, Metaphase I, Anaphase I, & Telophase I
  • Meiosis II stages are Prophase II, Metaphase II, Anaphase II, & Telophase II
  • Produces 4 haploid cells or gametes
  • When a sperm fertilizes an egg to form a zygote, the diploid number of chromosomes is restored (23 + 23 = 46)
  • Egg cells or ova (ovum, singular) are larger , nonmotile cells
  • Gametoogenesis is meiosis producing eggs & occurs in the female’s ovaries

 


Oogenesis

  • Sperms contain less cytoplasm so they’re smaller & have a flagellum to swim to the egg
  • Spermatogenesis is meiosis producing sperm cells & occurs in the testes

 


Spermatogenesis

Meiosis I:

  • The cell that undergoes Meiosis I is a primary spermatocyte or oocyte
  • Prophase I:
    • Chromosomes coil tightly & are visible
    • Nuclear membrane & nucleolus disintegrate
    • Spindle forms
    • Synapsis (joining) of homologous chromosomes occurs making tetrads
    • Kinetochore fiber forms on each chromosome
    • Chromosomes in tetrad exchange fragments by a process called crossing over

  • Metaphase I:
    • Tetrads become aligned in the center of the cell attached to spindle fibers
  • Anaphase I:
    • Homologous chromosomes separate
  • Telophase I:
    • May not occur in all species
    • Cytokinesis occurs producing 2 cells
    • In females,  2nd cell in females is called the 1st Polar Body
    • 1st Polar Body dies due to uneven splitting of the cytoplasm

  • Prophase II:
    • Cells called Secondary Spermatocytes or oocytes
    • DNA is not copied before cell divides
    • Chromatids attach to spindle fiber
  • Metaphase II:
    • Chromosomes become aligned in the center of the cell attached to spindle fibers
  • Anaphase II:
    • Sister chromatids separate randomly
    • Called independent assortment
  • Telophase I:
    • Cytokinesis occurs producing 4 cells in males called spermatids
    • Spermatids mature & form flagellum to become sperm
    • Cytokinesis in females produces a 2nd Polar Body that dies and an ootid 
    • Ootids mature to become ovum or egg

Asexual & Sexual reproduction:

  • Evolution is the slow process of change in living populations over time
  • Variations are differences that occur due to crossing-over among members of a sexually reproducing population
  • Variations are important to the survival of individuals in a population (some must survive to reproduce)
  • Asexually reproducing organisms rarely show variations because the organisms have identical genes

Cell Division Study Guide BI

Cell Division Study Guide

What molecule contains the information needed to direct all the activities of a cell?
Where in a cell are prokaryotic chromosomes found? eukaryotic chromosomes?
A human somatic cell contains how many homologous chromosomes?
How many chromosomes are in an human egg cell? sperm cell?
What is a karyotype?
Are gametes diploid or haploid?
Zygotes will have what chromosome number?
Does cell division in bacteria take place in the same way as it does in eukaryotes? Explain.
In what stage do cells spend most of their life cycle?
Is mitosis asexual or sexual reproduction?
A new nuclear envelope develops during cell division in what stage?
In what stage do chromatids separate from each other?
How does the number of chromosomes in newly divided cells compare with the number of chromosomes in the original cell?
During what type of cell division do haploid cells develop from diploid cells?
In order for DNA to fit into a cell, what must be done to compact it?
What is a centromere?
How many chromosomes are in a human skin cell? a human ovum?
Bacteria reproduce by a method known as _____________  ______________.
What is the shape of a bacterial chromosome?
Chromosomes are arranged along the equator of a cell during which stage of cell division?
Spindle fibers are made of ________________.
Be able to recognize sketches of the stages of mitosis.
What happens during cytokinesis in a plant cell?
Homologs separate during ________________.
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Catalase Bi Sample Lab 2

 

 

Enzyme Rate of Reaction for Catalase

 

Introduction:
Life would not be possible without chemical reactions. Chemical reactions are responsible for speeding up the process. A chemical reaction is the process of breaking chemical bonds, forming new bonds or both. The four things that can speed up a chemical reaction is heat, increasing the concentration of reactants, decreasing the concentration of products, and enzymes. Enzyme is a catalase, most the time a protein. Enzymes can control the rate of a reaction, and they also lower activation energy. Enzymes are important in regulating chemical pathways, synthesizing materials needed by cells, releasing energy, and transferring information. Enzymes are involved in digestion, respiration, vision, movement, and thought. There are several things that can affect the function of enzymes like temperature, the pH, and the amount of reactant or product. Simple cells may have as many as 2000 different enzymes, each one catalyzing a different reaction. In this particular lab, your hands act as the enzyme “Catalase”. This enzyme, which is found in your cells, splits hydrogen peroxide, a byproduct made by your cells during cellular respiration,  into water and oxygen.

 

Hypothesis:
If  time is increased, then more hydrogen peroxide molecules will be split into water and oxygen

 

Materials:
The materials used in this lab were pencils, scissors, envelope, 100 paper hydrogen peroxide molecules, and a watch with a second hand so that a person would be able to keep time for the person tearing the strips.

 

Methods:
Take a paper template and cut out 100 hydrogen peroxide molecules. Place the cut out pieces into an envelope. Then have a person act as a catalase and take one piece of the paper molecules out of the envelope at a time and rip it in two and  place the pieces back into the envelope. Have a person hold the envelope person, while another student keeps track of the “tearing” time intervals (10, 20, 30 ,60, and 60 seconds). Count how many molecules are  ripped at the end of each time interval and record this number in your data table. When all time intervals and counts are completed, use the formula below to figure the reaction rate for catalase. Record this rate in  your data table.
M2 – M1 = reaction rate
T2 – T1

Results:

 

 

Time in seconds

 

Ripped Hydrogen Peroxide Molecules

 

Rate of reaction

 

0-10

3 .3
 

10-30

10 .35
 

30-60

24 .47
 

60-120

63 .65
 

120-180

124 1.02

 

1. What is an enzyme? What are its functions in living things?
Enzymes are proteins in living systems. Enzymes can control the rate of a reaction, and they lower activation energy.

 

2. Name several things things that can affect the function of an enzyme?
Temperature, the amount of reactant or product and the pH.

3. Write the chemical equation for the breakdown of hydrogen peroxide by the enzyme catalase.
hydrogen peroxide + catalase yields water + oxygen

4. An enzyme’s efficiency increases with greater substrate concentration, but only up to a point. Why?
all of the active sites of the enzymes become filled with hydrogen peroxide molecules

 

5. If you were allowed to continue this lab and rip hydrogen peroxide molecules for 240 and 300 seconds. What would happen to the rate of reaction and why would this happen?
It would increase.

 

6. What can you say about the length of time and the rate of the reaction?
The less time, the more the reaction rate is lowered, and the more time, the more the reaction rate is higher.

 

7. What would happen to the rate of reaction if you remove the water  and oxygen molecules as soon as they are produced?
It would be faster.

 

Error Analysis:
All pieces must be returned to the envelope each time interval to correctly simulate what occurs within a cell.

 

Discussion and Conclusion:
As the time intervals increased, the reaction rate of catalase increased also. In a living cell, more hydrogen peroxide would be broken down by catalase over a longer period of time.

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