Starfish Dissection

 

Starfish Dissection

Introduction:

Echinoderms are radially symmetrical animals that are only found in the sea (there are none on land or in fresh water). Echinoderms mean “spiny skin” in Greek. Many, but not all, echinoderms have spiny skin. There are over 6,000 species. Echinoderms usually have five appendages (arms or rays), but there are some exceptions.

Radial symmetry means that the body is a hub, like a bicycle wheel, and tentacles are spokes coming out of it (think of a starfish). As larvae, echinoderms are bilaterally symmetrical. As they mature, they become radially symmetrical.

Most adult echinoderms live on the bottom of the ocean floor. Many echinoderms have suckers on the ends of their feet that are used to capture and hold prey, and to hold onto rocks in a swift current.

Sea Stars
Sea StarSea stars (group name Stelleroidea) are sometimes called starfish, though they are not real fish (they lack both vertebrae and fins). There are two sub-types of sea stars:

  • Asteroideas are the true sea stars and sun stars.
  • Ophiuroideas are brittle stars and basket stars.

The differences between the two sub-types lies in how the arms connect to the central disk. Ophiuroids have arms that do not connect with each other. There is a distinct boundary between arm and central disk. Asteroids have arms that are connected to each other. Also, it is harder to tell with asteroids where the central disk ends and the arms begin.

The sea star’s top surface (or skin) looks spiny if you examine it. If you look very closely you will notice that there are different types of growths on the surface. Some bumps are used to absorb oxygen, they are called dermal branchiae. Pedicellaria are pincher-like organs used to clean the surface of the skin. Barnacle larvae could land on a sea star and start growing if it were not for these organs.

How Do Sea Stars Move?
Underside of a Sea StarEach sea star had hundreds of tiny feet on the bottom of each ray. These are tube feet, or podia. These tiny feet can be filled with sea water. The vascular system of the sea star is also filled with sea water. By moving water from the vascular system into the tiny feet, the sea star can make a foot move by expanding it. This is how sea stars move around. Muscles within the feet are used to retract them.

Each ray of a sea star has a light sensitive organ called an eyespot. Though it can not see nearly as well as we do, sea stars can detect light and its general direction. They have some idea of where they are going.

Sea Star Anatomy

Can Sea Stars Grow New Arms?
Given enough time, sea stars can grow back arms that have been damaged or removed. For a few species, the severed arm can grow back into a complete sea star! For most sea stars, however, a severed limb dies.

What Do Sea Stars Eat?
Sea stars eat many things. A sea star’s diet can include: barnacles, snails, sea urchins, clams, and mussels. A few species, such as the spiny star of the North Atlantic, eat other sea stars! Many sea stars eat mussels and clams in an interesting way. They surround the shell and use the suckers on their feet to pull the two shells (or valves) apart. The sea star has enough force in its arms to actually bend the shell! This creates an opening between the two shells that is only .01 inches wide. Using this tiny gap, the sea star puts its stomach into the clam’s shell and eats its insides. When it is done, nothing is left but an empty shell.

Materials:

Preserved starfish, dissecting pan, scissors, scalpel, forceps, T-pins, pencil, lab apron, safety glasses

Procedure:

 

 

Dorsal view of starfish showing external anatomy

Ventral view of starfish showing external anatomy

Dorsal view of a dissected starfish showing rectal cecum, anus, madreporite, pyloric stomach, pyloric duct

Dorsal view of a dissected starfish showing madreporite, stone canal, cardiac stomach, and ampullae

Dissection showing where cardiac stomach opens into the mouth

Close up of madreporite and stone canal

Dorsal view of a dissected starfish showing pyloric caecum and pyloric ducts

Dorsal view of a dissected starfish showing gonads and ampullae

Ventral view of starfish showing external anatomy

Starfish Dissection

 

 

Starfish Dissection starfish anatomy

Introduction:

Echinoderms are radially symmetrical animals that are only found in the sea (there are none on land or in fresh water). Echinoderms mean “spiny skin” in Greek. Many, but not all, echinoderms have spiny skin. There are over 6,000 species. Echinoderms usually have five appendages (arms or rays), but there are some exceptions.

Radial symmetry means that the body is a hub, like a bicycle wheel, and tentacles are spokes coming out of it (think of a starfish). As larvae, echinoderms are bilaterally symmetrical. As they mature, they become radially symmetrical. Most adult echinoderms live on the bottom of the ocean floor. Many echinoderms have suckers on the ends of their feet that are used to capture and hold prey, and to hold onto rocks in a swift current.

Sea Stars
Sea StarSea stars (group name Stelleroidea) are sometimes called starfish, though they are not real fish (they lack both vertebrae and fins). There are two sub-types of sea stars:

  • Asteroideas are the true sea stars and sun stars.
  • Ophiuroideas are brittle stars and basket stars.

The differences between the two sub-types lies in how the arms connect to the central disk. Ophiuroids have arms that do not connect with each other. There is a distinct boundary between arm and central disk. Asteroids have arms that are connected to each other. Also, it is harder to tell with asteroids where the central disk ends and the arms begin. The sea star’s top surface (or skin) looks spiny if you examine it. If you look very closely you will notice that there are different types of growths on the surface. Some bumps are used to absorb oxygen, they are called dermal branchiae.  Pedicellaria are pincher-like organs used to clean the surface of the skin. Barnacle larvae could land on a sea star and start growing if it were not for these organs.

How Do Sea Stars Move?
Underside of a Sea StarEach sea star had hundreds of tiny feet on the bottom of each ray. These are tube feet, or podia. These tiny feet can be filled with sea water. The vascular system of the sea star is also filled with sea water. By moving water from the vascular system into the tiny feet, the sea star can make a foot move by expanding it. This is how sea stars move around. Muscles within the feet are used to retract them. Each ray of a sea star has a light sensitive organ called an eyespot. Though it can not see nearly as well as we do, sea stars can detect light and its general direction. They have some idea of where they are going.

Sea Star Anatomy

 

Prelab Questions (click here)

Materials:
Preserved starfish, dissecting pan, scissors, scalpel, forceps, T-pins, pencil, lab apron, safety glasses

Procedure (Aboral Surface):

  1. Obtain a preserved starfish and rinse off any preservative with water.
  2. Place the starfish in the dissecting pan with its dorsal or aboral (top) surface upward.
  3. Observe the starfish and determine  its symmetry.
  4. Locate the central disc in the center of the starfish. Count and record the number of arms or rays the starfish has.
  5. Locate the small, round hard plate called the madreporite on top of the central disc. Water enters through this into the water vascular system. Label the central disc, arms, and madreporite on Figure 1.
  6. Feel the upper surface of the starfish for spines. These spines protect the starfish and are part of their internal skeleton. Label these on figure 1.
  7. Look at the tip of each arm and find the eyespot. Label this on Figure 1.

Figure 1 -Aboral Surface

Procedure (Oral Surface):

  1. Turn the starfish over to its ventral or oral surface (underside).
  2. Locate the mouth in the center of the central disc. Find the ring of oral spines surrounding the mouth. Label these  on figure 2.
  3. Find the groove that extends down the underside of each arm. This is called the ambulacral groove. Label this on figure 2.
  4. Feel the numerous, soft tube feet inside each groove. these are part of the water vascular system & aid in movement and feeding. Label these on Figure 2.

Figure 2 – Oral Surface

Procedure (Internal anatomy):

  1. With the starfish’s aboral surface facing you, cut off the tip of a ray. Cut along lines a, b, and c (Figure 3) and then remove this flap of skin.

Figure 3 – Cuts in Arm

  1. Inside each arm, locate two long digestive glands called the pyloric caeca. These make enzymes to digest food in the stomach. Label these in Figure 4.
  2. Cut a circular flap of skin from the central disc. (You will have to also cut around the madreporite in order to remove this flap.) Observe the stomach under the central disc. Label this on Figure 4.
  3. Remove the pyloric caeca from the dissected ray. Find the gonads (testes or ovaries) underneath. These may be small if the starfish is NOT in breeding season. Label these on figure 4. Remove these to see the rest of the water vascular system.
  4. Cut off the tip of a ray to observe the parts of the tube feet. Find the zipper-like ridge that extends the length of the ray. The tube feet are attached to these.
  5. Locate the bulb-like top of a tube foot called the ampulla. This sac works like the top of an eyedropper to create suction. The bottom of the tube foot is a sucker. Label these in Figure 4.
  6. Embedded in the soft body wall are skeletal plates called ossicles. Locate these and label them in Figure 4.

Figure 4 – Starfish Digestive & Reproductive Systems

  1. Running down the center of each arm is a lateral canal to which tube feet are attached. Label this in Figure 5.
  2. In the central disc the five lateral canals connect to a circular canal called the ring canal. Find this canal & label it on figure 5.
  3. A short, canal called the stone canal leads from the ring canal to the madreporite where water enters. Find this canal & label the stone canal & madreporite on Figure 5.
  4. Draw an arrow on Figure 5 tracing the path that water takes when it enters & moves through the starfish.

Figure 5 – Water Vascular System

 

Starfish Anatomy Questions:

1. What type of symmetry did your starfish have?

2. What is the upper surface of the starfish called?

3. What is the lower surface of the starfish called?

 

4. On which surface are these parts of a starfish visible:

a. Mouth –

b. Madreporite –

c. Suckers –

d. Oral spines –

e.  Eyespots –

d. Ambulcaral groove –

5. In words, trace the path water takes through the water vascular system.

 

 

6. What part of the tube foot creates suction to open clams whenever the starfish feeds?

7. Why do the gonads sometimes appear larger?

8. What type of skeleton, endoskeleton or exoskeleton, does the starfish have?

9. What bony plates make up its skeleton?

10. What is the function of the pyloric caeca?

11. where is the stomach of a starfish located? What can the starfish do with its stomach when feeding on clams & oysters?

 

12. Name the kingdom, phylum, and class for the starfish you dissected.

 

Starfish Prelab

 

Starfish Prelab

1. In what phylum are starfish found?

2. What is the habitat for starfish?

3. On what do starfish feed?

4. What system in their body helps them catch & hold their food?

5. What does echinoderm mean in Greek? Why is this a good name for this group?

 

6. Name 2 classes of echinoderms & a member of each class.

 

7. Where does water enter a starfish? Where does it leave?

 

Strawberry DNA

 

Strawberry DNA Extraction


Adapted from a lab by C. Sheldon

Introduction:

DNA is found in cells from Animals and Plants.  DNA is a double stranded macromolecule composed of nucleotide bases pairing Adenine with Thymine and Guanine with Cytosine.  DNA can be extracted from cells by a simple technique with household chemicals, enabling students to see strands of DNA with the naked eye.

Purpose:

To extract DNA from the fruit of a strawberry plant

Safety Precautions:

  • Do not eat or drink in the laboratory.
  • Wear Apron & Safety Goggles.

Materials / Equipment (per student group):

1. heavy duty zip-lock baggie

2.  1 strawberry (fresh or frozen and thawed)

3.  cheesecloth

4.  funnel

5.  100 ml beaker

6.  test tube

7.  wooden coffee stirrer

8. DNA Extraction Buffer (One liter: mix 100 ml of shampoo (without conditioner), 15 g NaCl, 900 ml water OR 50 ml liquid dishwashing detergent, 15 g NaCl and 950 ml water)

9.  Ice-cold 95% ethanol or 95% isopropyl alcohol

Procedure:

1.  Place one strawberry in a zip lock baggie and carefully press out all of the air and seal the bag.

2.  Smash the strawberry with your fist for 2 minutes.

3.  Add 10 ml extraction buffer to the bag and carefully press out all of the air and seal the bag.

4.  Mush again for one minute.

5.  Filter through cheesecloth in a funnel into beaker. Support the test tube in a test tube rack.

6.  Discard the extra mashed strawberry.

7.  Pour filtrate into test tube so that it is 1/8 full.

8.  Slowly pour the ice-cold alcohol into the tube until the tube is half full and forms a layer over the top of the strawberry extract.

9.  At the interface, you will see the DNA precipitate out of solution and float to the top. You may spool the DNA on your glass rod or pipette tip.

10.                    Spool the DNA by dipping a pipette tip or glass rod into the tube right where the extract layer & alcohol are in contact with each other. With your tube at eye level, twirl the rod & watch as DNA strands collect.

Prelab:

Take a look at the sketch of the plant cell below. The chromosomes (which are made of DNA) are in the nucleus. This is the only place where DNA is located.

 

Now match the procedure with what it is doing to help isolate the DNA from the other materials in the cell.

 

_____1. Break open the cell A. Squish the fruit to a slush

 

_____2. Dissolve cell membranes B. Filter your extract through cheesecloth
_____3. Precipitate the DNA (clump the DNA together C. Mix in a detergent solution
_____4. Separate organelles, broken cell wall, and membranes from proteins, carbohydrates, and DNA D. Layer cold alcohol over the extract

 

 

DNA Extraction Table

AMOUNT ADDED OR OBTAINED INITIAL COLOR PURPOSE
BUFFER
(soap-salt mixture)
STRAWBERRY
COLD ALCOHOL
DNA

SKETCH OF TEST TUBE WITH CONTENTS

 

 

Questions:

1.  Where can DNA be found in the cell?

2.  Discuss the action of the soap (detergent) on the cell.  What is the purpose of the soap in this activity?

3.  What was the purpose of the Sodium Chloride? Include a discussion of polarity and charged particles.

4.  Why was the cold ethanol added to the soap and salt mixture?

5.  Describe the appearance of your final product?

6.  Draw a diagram of DNA containing 5 sets of nucleotide bases labeling the hydrogen bonds between the bases.

 

Biology Study Guides Summary of Links

Biology Study Guides
All Materials © Cmassengale

Safety & Equipment Chromosomes Flat & Round Worms Unsegmented Worm Review
Study of Life
Intro to Biology Review
Chapter 1 Introduction
Taxonomy
Taxonomy Review

Cladogram Practice
Mollusks
Mollusk Review
Chemistry

Chemistry Review

Evolution
Evolution Review
Annelids
Annelid Review
Biochemistry
Biochemistry Review
Viruses

Virus Review

Arthropods
Arthropod Review  
Cells
Cells – Units of Life
Cells & Their Functions
Cell Review

Cell Study Guide
Bacteria & Viruses
Bacteria & Viruses
Bacteria Review
Insects

Insect Review


Homeostasis & Transport

Handout – TRANSPORT
Cell Membrane Review
Transport Study Guide
Fungi
Fungi Review
Echinoderms
Echinoderm Review 
Photosynthesis
Photosynthesis Review
Protists
Protist Review
Fish
Fish Review  
Photosynthesis & Respiration

Photosynthesis & Cell Respiration

Mosses & Ferns Amphibians
Amphibian Review  
Cellular Respiration
Cell Respiration Review
Seed Plants Reptiles
Reptile Review  
Nucleic Acids
Nucleic Acid Review
Plant Structure & Function Birds
Birds Review 
Cell Growth & Division
Cell Cycle & Mitosis
Cell Reproduction Review
Introduction to Animals
Intro to Animals Review
Invertebrate Table
Mammals

Mammal Review  

Genetics
Genetics flashcards
Genetics Review
Sponges & Cnidarians
Review Worksheet
Ecology
Ecology Review

Cycles Worksheet  
Biogeochemical worksheet 
1st Semester 2003
2nd Semester  2003
1st Semester 2004
2nd Semester  2004
1st Semester 2006
1st Semester
2012

 

 

Biology I                


PreAP Biology