Safety Study Guide

 

 

Safety & Equipment Study Guide
Be able to recognize pictures of the following equipment:

dissecting pan
scalpel
probe
graduated cylinder
pipette
petri dish
test tube
depression slide
beaker
dissecting scissors
cover slips
Erlenmeyer flask
microscope slides
dissecting pins
test tube holder
forceps

Know the best solution for each of these safety problems:

Not being sure of what to do in a lab
An incorrect procedure causes a fire & explosion
Work area left in a messy condition for the next class
Long hair catches on fire
A chemical splashes into a student’s eye
A specimen slips when dissecting
An incorrect chemical is accidentally used from a bottle
A preservative  is ingested (taken into the mouth) from a dissecting specimen
Liquid spills while trying to label a beaker
The environment becomes polluted from lab wastes

 

Scientific Method & Blood Flow

 

Scientific Method & Blood

 

INTRODUCTION:

In this lab you will learn to form a hypothesis, conduct experiments around that hypothesis, and collect and analyze data. One of the most important characteristics of modern science is its quantitative approach to solving problems. One of the first scientists to use quantitative methods was William Harvey, who discovered that blood circulated through the body. At the time Harvey began his work, anatomists believed that the liver produced blood from the food that the body consumed. The blood was then carried by veins to the heart, purified in the lungs, and then pumped to the various organs of the body, where it was consumed. Harvey measured that the left ventricle of the heart held roughly 100 ml of blood. He also measured that the heart beats an average of 64 times per minute.

QUESTION 1:

From the information above, and assuming that 1 ml of blood weighs 1 g, how much blood would the body need to produce per hour in ( g/hr.) to replace the blood consumed by the organs? _______g/hr.

Harvey hypothesized that the same blood must circulate continuously throughout the body.

MATERIALS:

Watch with second hand, or clock

PROCEDURE:

  1. While sitting quietly at your desk, find the pulse in your wrist and count the beats for one minute. You and your lab partner can do this on yourselves, or each other. Record the names of both subjects and their beats per minute heart rate on DATA TABLE 1 as sample 1.
  2. Repeat step 1 two more times for each subject. Record the data in the appropriate place on DATA TABLE 1.
  3. Calculate the average pulse rate for each subject and record the results on DATA TABLE 1.

How do you think standing or holding your breath will affect your pulse rate? ______________________________________________________

QUESTION 2:

Choose one of these activities and formulate a hypothesis about its effect on pulse rate. What is the independent variable? What is the dependent variable?

Hypothesis _______________________________________________

Independent Variable _______________________________________

Dependent Variable ________________________________________

  1. Repeat steps 1, 2, and 3 for each subject, this time with the subjects standing or holding their breath. Record your data and calculations in the appropriate DATA TABLE

 

 

DATA TABLE 1: Resting heart rate
NUMBER OF BEATS PER MINUTE AVERAGE NUMBER OF
BEATS PER MINUTE
SUBJECT sample 1 sample 2 sample 3

 

 

 

DATA TABLE 2: Heart rate standing
NUMBER OF BEATS PER MINUTE AVERAGE NUMBER OF
BEATS PER MINUTE
SUBJECT sample 1 sample 2 sample 3

 

 

 

DATA TABLE 3: Heart rate holding breath
NUMBER OF BEATS PER MINUTE AVERAGE NUMBER OF
BEATS PER MINUTE
SUBJECT sample 1 sample 2 sample 3

 

Conclusion:  Compare your data from step 4 with your data from step 3.

1. How do your results in step 4 compare with the hypothesis you made?

 

2. What measurement did you use as a control in this investigation?

 

3. What are some possible sources of error in this experiment?

Salamander Key

 

 

Dichotomous Key to Salamanders

 

Introduction:

A dichotomous key is constructed of a series of couplets, each consisting of two separate statements. For example: couplet 1. Seeds round soybeans
1. Seeds oblong 2 (this statement indicates that you go to couplet “2”)

couplet 2. Seeds white northern beans
2. Seeds black black beans

By reading the two statements of each couplet, you progress through the key from typically broad characteristics to narrower characteristics until only a single choice remains. As long as the correct statement of each couplet is chosen, and the unknown organism is included in the key, a confident identification is usually achieved. Many types of organisms can be identified using a dichotomous key. In this lab, you will identify salamanders.

Materials:

pictures of various salamanders, dichotomous key, metric ruler, pencil

Procedure:

  1. Use the dichotomous key provided to identify the salamanders in Figure 1.
  2. Write the pathway you took to get to the name of the salamander next to the drawing.
  3. Write the correct name for the salamander on the line below each picture.

Figure 1 – Types of salamanders

Key to the Salamanders:

 

1 Hind limbs absent Siren
Hind limbs present Go to 2
2 External gills present in adults Mud puppy
External gills absent in adults Go to 3
3 Large size (over 7 cm long) Go to 4
Small size (under 7 cm long) Go to 5
4 Body background black, large white spots irregular in shape and size completely covering body & tail Tiger salamander
Body background black, small, round, white spots in a row along each side fro eye to tip of tail Spotted Salamander
5 Body background black with white spots Go to 6
Body background light color with dark spots and or lines on body Go to 7
6 Small white spots on a black background in a row along each side from head to tip of tail Jefferson salamander
Small white spots on a scattered throughout a black background from head to tip of tail Slimy salamander
7 Large irregular black spots on a light background extending from head to tip of tail Marbled salamander
b No large irregular black spots on a light background Go to 8
8 a Round spots scattered along back and sides of body, tail flattened like a tadpole Newt
b Without round spots and tail not flattened like a tadpole Go to 9
9 a Two dark lines bordering a broad, light mid-dorsal stripe with a narrow median dark line extending from the head onto the tail Two-lined salamander
b Without two dark lines running the length of the body Go to 10
10 a A light stripe running the length of the body and bordered by dark pigment extending downward on the sides Red-backed salamander
b A light stripe extending the length of the body, a marked constriction at the base of the tail Four-toed salamander

 

Scientific Method Notes

 

Scientific Method
All Materials © Cmassengale
How can we determine if something is a fact or an opinion? How can we determine an answer to a problem? The answer is use the scientific method.What is the Scientific Method? It is a series of steps used to help solve a problem.

  • Step 1. Make an Observation. After making an observation of the natural world, define the problem and make sure only one problem is being studied. ALL scientific experimentation starts with observation.
  • Step 2. Research the problem (question). Use all available resources to collect data on the subject being covered. Libraries, Internet, books, magazines, personal interviews, etc.
  • Step 3. Develop a hypothesis (educated guess). Make it a short definitive statement. It may be an “if” then” statement. The “if” part will become the hypothesis and the then part should be the results received at the end of the controlled experiment. Remember your hypothesis can be changed if the results do not support it.
  • Step 4. Develop a controlled experiment. A controlled experiment is an experiment that contains only one experimental variable. An experimental or independent variable is the thing being tested (what the scientist changes). Everything else in the experiment or all other variables must be the same. These variables are also called the controlled variables. Keeping these variables the same allows the experimenter to show that it was the experimental variable that caused the results. The dependent variable is what changes when the independent variable changes – the dependent variable depends on the outcome of the independent variable.  Data should be organized into charts, tables, or graphs.
  • Step 5. Analyze the data and come up with a conclusion. Data may be quantitative (numbers) or qualitative (appearance, properties, etc.).  The conclusion may or may not support the hypothesis. Additional experimentation must then take place to build documentation concerning the problem. If the hypothesis is proven wrong, change the hypothesis, not the data. Scientists must be unbiased.
  • WHAT FOLLOWS: Scientific research must be published, but first it must be reviewed by peers (other scientists) and verified for accuracy.  Research may result in a scientific theory or law.

Example:

Observation: Toaster stops working.
Question/Research: What is wrong with the toaster? (Read toaster Manual.)
Hypotheses: (1) It is unplugged. (2) The unit is burned out.
Experiments:  (1) Check the plug. (2) Take the toaster apart and look at the heating wires.
Results & Conclusion: If it was unplugged the first hypothesis is supported, if the wires inside are broken, then the second hypothesis is supported.

 

BACK

Puzzle Solution – Cell Reproduction

 

Cell Reproduction

Answer Key: (Students may have a different order.)

 

reproduction
sexual
asexual
chromosomes
histones
nonhistones
deoxyribonucleic
helix
supercoil
eukaryote
nucleus
nucleotides
autosomes
homologous
centromere
diploid
haploid
karyotype
fission
prokaryote
mitosis
meiosis
interphase
metaphase
prophase
anaphase
telophase
cytokinesis
centriole
spindle
cleavage
wall
gamete
synapsis
tetrad
assortment
oogenesis
spermatogenesis
spermatids
ootids