Starfish Dissection

 

 

Starfish Dissection starfish anatomy

Introduction:

Echinoderms are radially symmetrical animals that are only found in the sea (there are none on land or in fresh water). Echinoderms mean “spiny skin” in Greek. Many, but not all, echinoderms have spiny skin. There are over 6,000 species. Echinoderms usually have five appendages (arms or rays), but there are some exceptions.

Radial symmetry means that the body is a hub, like a bicycle wheel, and tentacles are spokes coming out of it (think of a starfish). As larvae, echinoderms are bilaterally symmetrical. As they mature, they become radially symmetrical. Most adult echinoderms live on the bottom of the ocean floor. Many echinoderms have suckers on the ends of their feet that are used to capture and hold prey, and to hold onto rocks in a swift current.

Sea Stars
Sea StarSea stars (group name Stelleroidea) are sometimes called starfish, though they are not real fish (they lack both vertebrae and fins). There are two sub-types of sea stars:

  • Asteroideas are the true sea stars and sun stars.
  • Ophiuroideas are brittle stars and basket stars.

The differences between the two sub-types lies in how the arms connect to the central disk. Ophiuroids have arms that do not connect with each other. There is a distinct boundary between arm and central disk. Asteroids have arms that are connected to each other. Also, it is harder to tell with asteroids where the central disk ends and the arms begin. The sea star’s top surface (or skin) looks spiny if you examine it. If you look very closely you will notice that there are different types of growths on the surface. Some bumps are used to absorb oxygen, they are called dermal branchiae.  Pedicellaria are pincher-like organs used to clean the surface of the skin. Barnacle larvae could land on a sea star and start growing if it were not for these organs.

How Do Sea Stars Move?
Underside of a Sea StarEach sea star had hundreds of tiny feet on the bottom of each ray. These are tube feet, or podia. These tiny feet can be filled with sea water. The vascular system of the sea star is also filled with sea water. By moving water from the vascular system into the tiny feet, the sea star can make a foot move by expanding it. This is how sea stars move around. Muscles within the feet are used to retract them. Each ray of a sea star has a light sensitive organ called an eyespot. Though it can not see nearly as well as we do, sea stars can detect light and its general direction. They have some idea of where they are going.

Sea Star Anatomy

 

Prelab Questions (click here)

Materials:
Preserved starfish, dissecting pan, scissors, scalpel, forceps, T-pins, pencil, lab apron, safety glasses

Procedure (Aboral Surface):

  1. Obtain a preserved starfish and rinse off any preservative with water.
  2. Place the starfish in the dissecting pan with its dorsal or aboral (top) surface upward.
  3. Observe the starfish and determine  its symmetry.
  4. Locate the central disc in the center of the starfish. Count and record the number of arms or rays the starfish has.
  5. Locate the small, round hard plate called the madreporite on top of the central disc. Water enters through this into the water vascular system. Label the central disc, arms, and madreporite on Figure 1.
  6. Feel the upper surface of the starfish for spines. These spines protect the starfish and are part of their internal skeleton. Label these on figure 1.
  7. Look at the tip of each arm and find the eyespot. Label this on Figure 1.

Figure 1 -Aboral Surface

Procedure (Oral Surface):

  1. Turn the starfish over to its ventral or oral surface (underside).
  2. Locate the mouth in the center of the central disc. Find the ring of oral spines surrounding the mouth. Label these  on figure 2.
  3. Find the groove that extends down the underside of each arm. This is called the ambulacral groove. Label this on figure 2.
  4. Feel the numerous, soft tube feet inside each groove. these are part of the water vascular system & aid in movement and feeding. Label these on Figure 2.

Figure 2 – Oral Surface

Procedure (Internal anatomy):

  1. With the starfish’s aboral surface facing you, cut off the tip of a ray. Cut along lines a, b, and c (Figure 3) and then remove this flap of skin.

Figure 3 – Cuts in Arm

  1. Inside each arm, locate two long digestive glands called the pyloric caeca. These make enzymes to digest food in the stomach. Label these in Figure 4.
  2. Cut a circular flap of skin from the central disc. (You will have to also cut around the madreporite in order to remove this flap.) Observe the stomach under the central disc. Label this on Figure 4.
  3. Remove the pyloric caeca from the dissected ray. Find the gonads (testes or ovaries) underneath. These may be small if the starfish is NOT in breeding season. Label these on figure 4. Remove these to see the rest of the water vascular system.
  4. Cut off the tip of a ray to observe the parts of the tube feet. Find the zipper-like ridge that extends the length of the ray. The tube feet are attached to these.
  5. Locate the bulb-like top of a tube foot called the ampulla. This sac works like the top of an eyedropper to create suction. The bottom of the tube foot is a sucker. Label these in Figure 4.
  6. Embedded in the soft body wall are skeletal plates called ossicles. Locate these and label them in Figure 4.

Figure 4 – Starfish Digestive & Reproductive Systems

  1. Running down the center of each arm is a lateral canal to which tube feet are attached. Label this in Figure 5.
  2. In the central disc the five lateral canals connect to a circular canal called the ring canal. Find this canal & label it on figure 5.
  3. A short, canal called the stone canal leads from the ring canal to the madreporite where water enters. Find this canal & label the stone canal & madreporite on Figure 5.
  4. Draw an arrow on Figure 5 tracing the path that water takes when it enters & moves through the starfish.

Figure 5 – Water Vascular System

 

Starfish Anatomy Questions:

1. What type of symmetry did your starfish have?

2. What is the upper surface of the starfish called?

3. What is the lower surface of the starfish called?

 

4. On which surface are these parts of a starfish visible:

a. Mouth –

b. Madreporite –

c. Suckers –

d. Oral spines –

e.  Eyespots –

d. Ambulcaral groove –

5. In words, trace the path water takes through the water vascular system.

 

 

6. What part of the tube foot creates suction to open clams whenever the starfish feeds?

7. Why do the gonads sometimes appear larger?

8. What type of skeleton, endoskeleton or exoskeleton, does the starfish have?

9. What bony plates make up its skeleton?

10. What is the function of the pyloric caeca?

11. where is the stomach of a starfish located? What can the starfish do with its stomach when feeding on clams & oysters?

 

12. Name the kingdom, phylum, and class for the starfish you dissected.

 

Starfish Prelab

 

Starfish Prelab

1. In what phylum are starfish found?

2. What is the habitat for starfish?

3. On what do starfish feed?

4. What system in their body helps them catch & hold their food?

5. What does echinoderm mean in Greek? Why is this a good name for this group?

 

6. Name 2 classes of echinoderms & a member of each class.

 

7. Where does water enter a starfish? Where does it leave?

 

Stemcell Article

Stem Cell Research Basics: Introduction

Stem cells in the human body have a unique ability to renew themselves and give rise to the more specialized cell types that do the work of the body. Stem cells remain unspecialized until a signal from the body tells them to develop into specific cells of the body like a heart, nerve, or skin cell.

illustration of stem cells to differentiated cells

For years, researchers have been studying the unique characteristics of stem cells. The first stem cells studied by researchers were derived from adult tissues and, more recently, scientific breakthroughs have enabled research on stem cells that are removed from one of the earliest human cellular formations, the blastocyst.

 

What is a stem cell?

All stem cells, no matter their source, are unspecialized cells that give rise to more specialized cells. Stem cells can become one of more than 200 specialized cells in the body. They serve as the body’s repair system by renewing themselves and replenishing more specialized cells in the body.

Background: SCNT

What do stem cells look like under the microscope?

stem cell colonies  stem cell colonies
Early human stem cells

How many types of stem cells are there?

The easiest way to categorize stem cells is by dividing them into two types: mature and early. Mature stem cells are found in specific mature body tissues as well as the umbilical cord and placenta after birth. Early stem cells, often called embryonic stem cells, are found in the inner cell mass of a blastocyst after approximately five days of development. See the below tables for more details on the characteristics of mature and early stem cells.

chart comparing mature versus early stem cells

What are the advantages and disadvantages of mature and early human stem cells?

We still have a great deal to learn about both mature and early human stem cells and their potential for treating and hopefully curing disease. Both have distinct advantages and disadvantages associated with them and each offer important insight into how cells rejuvenate the body and cause disease. See the below table for more information:

chart comparing Advantages and Disadvantages of Mature versus Early stem cells

Stem Cell Research Basics: Mature Stem Cells

What is a mature stem cell?

Stem cells are associated with most tissues of the body as part of a tissue/cell renewal mechanism — how the body regenerates its tissues. What is known to date is that mature stem cells are primarily multipotent, meaning they can yield all of the cell types associated with the tissues from which they originate. The mature stem cell is an undifferentiated (unspecialized) cell that is found in a differentiated (specialized) tissue, which can renew itself for a lifetime.

multipotent stem cell graphic

What is the role of mature stem cells?

Mature stem cells maintain and repair the body’s tissues in which they are found.

Can mature stem cells become any type of body cell?

Traditionally, mature stem cells have been considered limited in their potential to become any type of body cell. In other words, they only produce cell varieties within their own lineage or type and are considered multipotent. For example, stem cells found in bone marrow can become bone as well as cartilage, fat cells, various kinds of muscle, and the cells that line blood vessels.

Can mature stem cells be pluripotent?

Unlike early stem cells, there is no evidence to date that any mature stem cells are capable of forming all cells of the body. However, recent studies have demonstrated that mature stem cells may be more flexible than previously thought. More studies are necessary to validate these results.

Where can mature stem cells be found?

Sources of mature stem cells have been found in areas of the body including bone marrow, blood stream, cornea and retina of the eye, the dental pulp of the tooth, liver, skin, gastrointestinal tract, and pancreas.

What is the most common type of mature stem cell used today?

The mature stem cells associated with those that form blood in bone marrow are the most common type of stem cell used to treat human diseases today.

bone marrow stem cell graphic

How do mature stem cells treat diseases like cancer?

For more than 30 years, bone marrow stem cells have been used to treat cancer patients with conditions like leukemia and lymphoma. During chemotherapy, most of the leukemia cells are killed as are the bone marrow stem cells needed as a patient recovers. However, if stem cells are removed before chemotherapy, and then re-injected after treatment is completed, the stem cells in the bone marrow are able to produce large amounts of red and white blood cells, to keep the body healthy and to help fight infections.

bone marrow stem cell graphic

Stem Cell Research Basics: Early Stem Cells

What is an early stem cell?

Often called embryonic stem cells, early stem cells are found in the earliest human cellular formations. In normal human reproductive development early stem cells become all the cells of the body, making them pluripotent. When extracted from the inner cell mass of a blastocyst, early stem cells grow easily in cell culture and can multiply into many stem cell lines that can be maintained for a long period of time. Early human stem cells are thought to be an important source of potentially all types of clinically relevant cells that could become replacement tissue (e.g., organs) in the future or be used to treat and prevent disease.

What is a blastocyst?

A blastocyst is a thin-walled hollow sphere made up of an outer layer of cells, a fluid filled cavity, and an inner cell mass containing pluripotent stem cells. Also called the blastula, the blastocyst develops after cleavage and prior to implantation at approximately five days. Further reproductive development occurs only if the blastocyst is successfully implanted in the uterus.

blastocyst illustration and microscopic view

How big is a blastocyst?

A blastocyst is a microscopic group of cells — smaller than Roosevelt’s eye on the face of a U.S. dime. At day five when stem cells can be harvested, a blastocyst is approximately 0.1 to 0.2 millimeters (0.0039 to 0.0079 inches) in size.

graphic of ruler with dime and blastocyst

Where do stem cells come from?

Early stem cells are found in the inner cell mass of the blastocyst. Once the inner cell mass is removed from the blastocyst, the stem cells are placed in a culture dish where they grow and replicate over time. In normal reproductive development, the inner cell mass becomes all the tissues of the body and the yolk sac.

graphic of Blastocyst with inner cell mass and Petri dish

What is the primary source of blastocysts for research?

The primary sources of blastocysts for use in stem cell research are pre-embryos created by the in vitro fertilization process and donated by consenting adults once they are no longer needed for reproductive purposes.

When were early human stem cells first isolated from IVF-blastocysts?

Early human stem cells were first isolated in 1998 by Dr. James Thomson and his research team at the University of Wisconsin. For more information on Dr. Thomson’s research please visit his web site at the University of Wisconsin. Between 1998 and 2001, approximately 78 early stem cell lines have been created using IVF-blastocysts globally. For a table with information on the 78 stem cell lines, click here.

IVF graphic

How many unwanted pre-embryos are there in the United States?

According to a survey conducted in 2003, there are approximately 400,000 unwanted pre-embryos in the United States. (Source: Hoffman, D.I., et al. 2003. Cryopreserved embryos in the United States and their availability for research. Fertility and Sterility 79: 1063-1069.) These pre-embryos are no longer needed for fertility purposes and remain frozen or will be destroyed.

Are there any new sources of blastocysts for research that do not involve a fertilized egg?

Somatic cell nuclear transfer (SCNT) was successfully employed in 2004 to create the first human blastocyst from an unfertilized egg for the purposes of extracting early stem cells for research. Prior to 2004, SCNT was only used for animal research.

What is Somatic Cell Nuclear Transfer (SCNT)?

Somatic cell nuclear transfer (SCNT) is a laboratory procedure that produces a blastocyst from an unfertilized egg and an ordinary adult somatic cell (e.g., from a single skin cell). While SCNT techniques have been utilized in animal research for many years, human pluripotent stem cells were only first derived from SCNT-blastocysts in 2004 by a South Korean research team.

How does SCNT work?

SCNT substitutes the nucleus of a somatic cell (which contains all the genetic information of the patient) for the nucleus of a donated egg that has not been fertilized. In cell culture, this customized egg is then coaxed with an electronic or chemical catalyst to develop into a zygote as if it had been fertilized. The zygote begins cell division and develops into a ball of cells called the morula and then into the blastocyst at approximately five days. The inner cell mass of the blastocyst is then removed to generate a pluripotent stem cell line. After the inner cell mass is removed, the blastocyst is no longer capable of further development.

SCNT graphic

When can we expect results from SCNT?

The SCNT methodology is still in its infancy. Researchers hypothesize that when the genetic information from the cells of a patient is used, the pluripotent stem cells will be able to make customized tissue that will not be rejected by the patient. SCNT researchers contend that the knowledge gained about developmental biology via the SCNT methodology will allow future researchers to create individualized pluripotent stem cell lines without needing fertilized eggs as sources.

Can SCNT be used to clone humans?

The purpose of SCNT is to find cures and therapies to treat human disease. SCNT awakens the natural capacity for self-repair that resides in a person’s genes. While SCNT has been the technique used to clone animals like “Dolly” the sheep, there is no evidence that it could also successfully clone a human due to the increased complexity of the human organism.

Blastocysts produced by a fertilized egg (IVF) and SCNT are considered by many to be fundamentally different, and no SCNT-blastocysts should ever be implanted in a uterus. There is no conception of new life via SCNT.

For more information about SCNT, please visit:

Stem Cell Research Basics: Promise of Stem Cell Research

Potential U.S. Patient Populations for Stem Cell-Based Therapies Despite the biomedical research advances of the last 50 years, much is still left to be discovered in human biology and millions of people still suffer from devastating diseases. Early human stem cell research is viewed by many as a key to understanding many of the most fundamental questions in basic and clinical biology that can lead to treatments and cures, and ultimately save lives.

With a better understanding of early cell development, researchers expect to increase their knowledge of why cells behave abnormally and produce diseases such as cancer. Research on early human stem cells may also reduce a number of barriers posed by animal studies, which can be a major limitation in getting new therapies to market.

It is expected that early human stem cells could be used to create an unlimited supply of cells, tissues, or even organs that could be transplanted to restore function lost to disease and injury. While early stem cell research in humans is still in its infancy and specific treatments have not yet been developed, many experts expect treatments will be possible in the future for the following types of illnesses, injuries, and diseases:

  • Type 1 Diabetes in children
  • Nervous system diseases, such as Parkinson’s and Alzheimer’s, and spinal cord injuries
  • Primary immunodeficiency disease
  • Diseases of bone and cartilage
  • Cancer.

Research Results

While no early stem cell therapies are being used in humans yet, a number of recent research studies demonstrate success in animals. Some of those research efforts include:

Spinal Cord Injury:

McDonald, J.W., et. al. Transplanted embryonic stem cells survive, differentiate, and promote recovery in injured rat spinal cord. Nature Medicine 5: 1410-1412 (01 Dec 1999).

Heart Disease:

Fraidenraich, D., et al. Rescue of Cardiac Defects in Id Knockout Embryos by Injection of Embryonic Stem Cells. Science 306: 247-252 (8 October 2004).

Menasche, P. 2004. Embryonic stem cells pace the heart. Nature Biotechnology 22: 1237-1238 (01 October 2004).

Parkinson’s Disease:

Takagi, Y., et al. 2005. Dopaminergic neurons generated from monkey embryonic stem cells function in a Parkinson primate model. J. Clin. Invest. 115: 102-109.

ALS (Lou Gehrig’s Disease):

Wichterie, H., et al. 2002. Directed differentiation of embryonic stem cells into motor neurons. Cell 110: 385-397.

 

Article Citation

Thomson, Dr. James. “Stem Cell research basics: Introduction.” University of Wisconsin-Madison. 1998. <http://www.news.wisc.edu/packages/stemcells/facts.html#1.>

Strawberry DNA

 

Strawberry DNA Extraction


Adapted from a lab by C. Sheldon

Introduction:

DNA is found in cells from Animals and Plants.  DNA is a double stranded macromolecule composed of nucleotide bases pairing Adenine with Thymine and Guanine with Cytosine.  DNA can be extracted from cells by a simple technique with household chemicals, enabling students to see strands of DNA with the naked eye.

Purpose:

To extract DNA from the fruit of a strawberry plant

Safety Precautions:

  • Do not eat or drink in the laboratory.
  • Wear Apron & Safety Goggles.

Materials / Equipment (per student group):

1. heavy duty zip-lock baggie

2.  1 strawberry (fresh or frozen and thawed)

3.  cheesecloth

4.  funnel

5.  100 ml beaker

6.  test tube

7.  wooden coffee stirrer

8. DNA Extraction Buffer (One liter: mix 100 ml of shampoo (without conditioner), 15 g NaCl, 900 ml water OR 50 ml liquid dishwashing detergent, 15 g NaCl and 950 ml water)

9.  Ice-cold 95% ethanol or 95% isopropyl alcohol

Procedure:

1.  Place one strawberry in a zip lock baggie and carefully press out all of the air and seal the bag.

2.  Smash the strawberry with your fist for 2 minutes.

3.  Add 10 ml extraction buffer to the bag and carefully press out all of the air and seal the bag.

4.  Mush again for one minute.

5.  Filter through cheesecloth in a funnel into beaker. Support the test tube in a test tube rack.

6.  Discard the extra mashed strawberry.

7.  Pour filtrate into test tube so that it is 1/8 full.

8.  Slowly pour the ice-cold alcohol into the tube until the tube is half full and forms a layer over the top of the strawberry extract.

9.  At the interface, you will see the DNA precipitate out of solution and float to the top. You may spool the DNA on your glass rod or pipette tip.

10.                    Spool the DNA by dipping a pipette tip or glass rod into the tube right where the extract layer & alcohol are in contact with each other. With your tube at eye level, twirl the rod & watch as DNA strands collect.

Prelab:

Take a look at the sketch of the plant cell below. The chromosomes (which are made of DNA) are in the nucleus. This is the only place where DNA is located.

 

Now match the procedure with what it is doing to help isolate the DNA from the other materials in the cell.

 

_____1. Break open the cell A. Squish the fruit to a slush

 

_____2. Dissolve cell membranes B. Filter your extract through cheesecloth
_____3. Precipitate the DNA (clump the DNA together C. Mix in a detergent solution
_____4. Separate organelles, broken cell wall, and membranes from proteins, carbohydrates, and DNA D. Layer cold alcohol over the extract

 

 

DNA Extraction Table

AMOUNT ADDED OR OBTAINED INITIAL COLOR PURPOSE
BUFFER
(soap-salt mixture)
STRAWBERRY
COLD ALCOHOL
DNA

SKETCH OF TEST TUBE WITH CONTENTS

 

 

Questions:

1.  Where can DNA be found in the cell?

2.  Discuss the action of the soap (detergent) on the cell.  What is the purpose of the soap in this activity?

3.  What was the purpose of the Sodium Chloride? Include a discussion of polarity and charged particles.

4.  Why was the cold ethanol added to the soap and salt mixture?

5.  Describe the appearance of your final product?

6.  Draw a diagram of DNA containing 5 sets of nucleotide bases labeling the hydrogen bonds between the bases.

 

Biology Study Guides Summary of Links

Biology Study Guides
All Materials © Cmassengale

Safety & Equipment Chromosomes Flat & Round Worms Unsegmented Worm Review
Study of Life
Intro to Biology Review
Chapter 1 Introduction
Taxonomy
Taxonomy Review

Cladogram Practice
Mollusks
Mollusk Review
Chemistry

Chemistry Review

Evolution
Evolution Review
Annelids
Annelid Review
Biochemistry
Biochemistry Review
Viruses

Virus Review

Arthropods
Arthropod Review  
Cells
Cells – Units of Life
Cells & Their Functions
Cell Review

Cell Study Guide
Bacteria & Viruses
Bacteria & Viruses
Bacteria Review
Insects

Insect Review


Homeostasis & Transport

Handout – TRANSPORT
Cell Membrane Review
Transport Study Guide
Fungi
Fungi Review
Echinoderms
Echinoderm Review 
Photosynthesis
Photosynthesis Review
Protists
Protist Review
Fish
Fish Review  
Photosynthesis & Respiration

Photosynthesis & Cell Respiration

Mosses & Ferns Amphibians
Amphibian Review  
Cellular Respiration
Cell Respiration Review
Seed Plants Reptiles
Reptile Review  
Nucleic Acids
Nucleic Acid Review
Plant Structure & Function Birds
Birds Review 
Cell Growth & Division
Cell Cycle & Mitosis
Cell Reproduction Review
Introduction to Animals
Intro to Animals Review
Invertebrate Table
Mammals

Mammal Review  

Genetics
Genetics flashcards
Genetics Review
Sponges & Cnidarians
Review Worksheet
Ecology
Ecology Review

Cycles Worksheet  
Biogeochemical worksheet 
1st Semester 2003
2nd Semester  2003
1st Semester 2004
2nd Semester  2004
1st Semester 2006
1st Semester
2012

 

 

Biology I                


PreAP Biology