Category: Chemistry

Water Properties Handout

Physical Properties of Water

All of water’s unique physical properties are caused by water’s polarity.

  1. Cohesion: water molecules stick to each other.  This is caused by hydrogen bonds that form between the slightly positive and negative ends of neighboring molecules.  This is the reason why water is found in drops; perfect spheres.  It’s hard to imagine water behaving any other way.

  1. Adhesion: water molecules stick to other surfaces.  This causes water to move upward against gravity in plant stems and to be absorbed by paper towels.  It also causes water to adhere to spider webs.

  1.   Surface Tension: water has the ability to support small objects.  The hydrogen bonds between neighboring molecules cause a “film” to develop at the surface.

 

Organisms like the water strider can be seen taking advantage of this property.

 

4.  Water has a high boiling point.  Water is one of the few substances that remain a liquid at such a large range of temperatures (O-100 °C).  A large amount of energy must be invested to overcome the hydrogen bonds in liquid water to change it to the gas phase.

 

                            

Liquid Water Molecules with hydrogen bonds                              Water Vapor Molecules

5.  Capillary Action: water has the ability to “climb” structures.  Think about what happens when you stick the tip of a straw in a glass of water.

6.  Ability to Dissolve: water is consider to be the universal solvent.  More substances will dissolve in water than any other liquid.  This includes other polar substances (such as sugar) and ionic compounds (such as salt).

 

When a sugar crystal is placed in water, the slightly positive and negative ends of the water molecule attract the sugar molecules in the crystal (they are also polar) and pull them into solution.

 

When an salt crystals are placed in water, the slightly positive and negative ends of the water molecules attract the ions in the crystal.  The ionic bonds holding the sodium and chlorine ions together are broken and the ions are pulled into solution.

 

7. High Heat of Vaporization:

Water Properties Prelab

 

 

Properties of Water

Pre-Lab Questions:

1. Explain why water is referred to as the universal solvent.

 

2. What is the overall charge on a molecule of water?

3. Water is a polar molecule (appears to have a charge). Explain why this is so.

 

4. Which end of a water molecule “acts negative”? Which “acts positive”?

5. Is water the only molecule that is polar?

6. Explain what occurs whenever several water molecules are near each other in a droplet. Include a sketch of this.

 

 

 

7. The property of water molecules being attracted to other water molecules is called ________________.

8. Explain what causes water to have surface tension.

 

9. Surface tension causes causes water to _____________ on surfaces such as glass.

10. In order to clean a surface, what must happen to surface tension? What type of chemicals can do this? Give an example

 

11. Besides reducing surface tension, what 4 other things can surfactants perform?

 

 

Sucrose Hydolysis by Sucrase

 

Sucrose Hydrolysis Using Sucrase

INTRODUCTION:

In this lab, you will demonstrate the production of the enzyme sucrase (invertase) by yeast. The enzyme sucrase catalyzes the hydrolysis of the disaccharide sucrose to invert sugar. Invert sugar is a mixture of glucose and fructose, which are both monosaccharides. Yeast cannot directly metabolize (ferment) sucrose. For the yeast to utilize sucrose as an energy source, it must first convert it to the fermentable monosaccharides glucose and fructose.
Benedict’s solution is a test reagent that reacts positively with simple reducing sugars. All monosaccharides and most disaccharides are reducing sugars, possessing a free carbonyl group (=C=O). Sucrose is an exception in that it is not a reducing sugar. A positive Benedict’s test is observed as the formation of a brownish-red cuprous oxide precipitate. A weaker positive test will be yellow to orange. Both glucose and fructose test positive with benedict’s solution, sucrose does not.

MATERIALS NEEDED:

*Yeast filtrate solution
one 7 gram package active dry yeast per 80 mL distilled water
Ring stand and ring to hold funnel
Filtering funnel
Filter paper (fast speed)
**5% sucrose solution
5 grams sucrose per 95 mL distilled water
***5% glucose (dextrose) solution
5 grams dextrose per 95 mL distilled water
Benedict’s qualitative solution
Distilled water
Five 10-mL graduated cylinders (one for each solution)
7 test tubes 18 x 150 mm
Test tube holder
Test tube rack
2 400-mL beakers
Hot plate

PRE-LAB:

* To prepare a yeast filtrate solution, mix one package of active dry yeast with 80 mL of distilled water. Let stand for 20 minutes, stirring occasionally. Filter the resulting suspension and save the filtrate solution. This is your invertase extract. Refrigerate the extract if held overnight. Approximately enough for 8 labs.

**To prepare a 5% sucrose solution, dissolve 5 grams of sucrose in 95 mL of distilled water. This should be prepared shortly before use. Approximately enough for 4 labs.

***To prepare a 5% glucose solution, dissolve 5 grams of dextrose (this is the name used when dry) in 95 mL of distilled water. This should be prepared shortly before use. Approximately enough for 9 labs.

PROCEDURE:

1. Label 3 test tubes A1, A2, and A3, and place in the test tube rack. Place into the test tubes as follows:

    • Into tube

A1

    • , place

10 mL

    • of 5% sucrose solution.

Into tube A2, place 10 mL of 5% sucrose solution and 4 mL of invertase extract.

Into tube A3, place 10 mL distilled water and 4 mL of invertase extract.

Thump the tubes to mix.

2. Put approximately 250 mL of 30 to 35 oC water into a 400-mL beaker. Incubate the three tubes in this warm water bath for 35 minutes.

3. Label 4 test tubes B1, B2, B3 and B4, and place in the test tube rack. Place 5 mL of Benedict’s qualitative solution into each tube. Now transfer to the B tubes as follows:

    • Into tube

B1

    • , transfer the contents of tube

A1

    • .

Into tube B2, transfer the contents of tube A2.

Into tube B3, transfer the contents of tube A3.

Into tube B4, place 10 mL of 5% glucose solution.

Thump the tubes to mix.

4. Place tubes B1, B2, B3, and B4 into a boiling water bath. CAUTION: do not let the bath boil hard. Keep it just at the boiling point. After 3 or 4 minutes, remove the tubes and note whither any change is evident.

QUESTIONS AND OBSERVATIONS:

1. Did tube B1 test positive or negative?

2. What does this show?

 

3. Did tube B2 test positive or negative?

4. What does this show?

5. Did tube B3 test positive or negative?

6. What does this show?

 

7. Did tube B4 test positive or negative?

8. What does this show?