Calorimetry lab

Calorimetry – Measuring the energy in Foods

Introduction:
There are two processes that organisms use to make usable energy. The process by which autotrophs convert sunlight to a usable form of energy is called photosynthesis. Photosynthesis supports all life on earth. Products from photosynthesis include food, textiles, fuel, wood, oils, and rubber. During photosynthesis, light energy is used to make organic compounds from inorganic water and carbon dioxide. Photosynthesis goes through light dependent reactions and the light independent reactions which include the Calvin cycle.
The process where heterotrophs break down food molecules to release energy for work is called cellular respiration. Cellular respiration is the reverse of photosynthesis; the reactants of one are the products of the other. The reactants of cellular respiration are glucose and oxygen, and the products are carbon dioxide, water, and energy.  Cellular respiration breaks down glucose to form carbon dioxide and water, while releasing energy usable by the cells. The first step, glycolysis is the process  that converts glucose to pyruvate and releases a small amount of cellular energy.  The second step may be aerobic or anaerobic depending on the amount of oxygen available.  Aerobic respiration is the breakdown of pyruvate in the presence of oxygen.  A larger amount of cellular energy or ATP is produced during the Kreb’s cycle and electron transport chain. Anaerobic respiration is the breakdown of food molecules in the absence of oxygen. Less ATP is produced by anaerobic respiration or fermentation.

Hypothesis:
If the heat given off by a burning pecan is measured by how much the temperature increases in a given amount of water, then the number of calories of energy stored in the nut during photosynthesis can be determined.

Materials:
Items needed for the lab included a large paper clip, a 100 ml graduated cylinder, thermometer, 2 soft drink cans, electronic balance, butane lighter, plastic tray, scissors, paper, and pencil.

Procedure:
Use a graduated cylinder to measure 100 ml of water and add this to an empty soft drink can. Cut holes on two sides of a second soft drink can so there is room to place a large bent paper clip.  Measure and record the mass of one pecan using the electronic balance. Bend a large paper clip to make a “nut stand” and measure and record  the mass of this clip. Place the pecan on the nut stand and put the stand inside the cut-out drink can.  Use a thermometer to measure and record the temperature of the water in the second can.  Place this can on top of the can with the nut. Use a butane lighter to ignite the nut. Record the temperature of the water when the nut is completely burned. Complete the data table by calculating the  the total calories in the pecan.

Data:    

Data Table 1

Before Burning After Burning Difference
Mass of Nut 1.7 g 0.1g 1.6g
Temperature of Water 20 40.1 20.1
Mass of Paper Clip 1.4g 1.4g 0g

 

Data Table 2
Mass of pecan 0.1 g
Temperature change of 100 ml of water 20.1
Calories required to produce temperature change in 100 ml water 2010
Calories per gram contained in the pecan 1182.4

Error Analysis:
Errors may have occurred in several ways during this experiment. One error that may have occurred is that some of the energy may have been lost during the burning. Some of the pecan’s energy was lost as light instead of heat energy. Also some of the heat measured in the water could have been due to the butane lighter used to ignite the pecan.

Conclusion:
The temperature of the 100 ml of water in the can above the burning pecan was changed by the energy given off by the pecan when it was burned.  The energy given off by the burning pecan was great enough to increase the water temperature by 20.1 degrees Celsius. The mass of the unburned pecan was 1.7g. It takes 100 calories to raise the temperature of 1 ml of water by 1 degree Celsius. The temperature of 100 ml of water was recorded to have increased by 20.1 degrees Celsius; therefore, the total number of calories in the pecan equals 20.1 x 100 or 2010 calories. Since the nut had a mass of 1.7g, the number of calories per gram equals 2010 divided by 1.7 or 1182.4 calories per gram.
The increase of temperature in the water showed that energy had been stored in the pecan. In this experiment, the amount of calories of heat energy stored in a pecan during photosynthesis was measured by a process known as calorimetry.

 

Campbell Problem 7

Molecular Genetics Problem 7
7. Using the information from problem 6, a further testcross was done using a heterozygote for height and nose morphology. The offspring were tall-upturned nose, 40; dwarf-upturned nose, 9; dwarf-downturned nose, 42; tall-downturned nose, 9. Calculate the recombination frequency from these data; then use your answer from problem 6 to determine the correct sequence of the three linked genes.

Experiment 3. (Frequency/Distance between T and S)

Determine the recombination frequency for the genes controlling Tallness and Snout:

40 tall-upturned snout = 40% expected
42 dwarf-downturned snout = 42% expected
9 dwarf-upturned snout = 9% recombinant
9 tall-downturned snout = 9% recombinant

Total = 100%

Therefore this recombination frequency between genes T and S is 18%

One can determine the relative frequency between genes using the percent frequencies as distances.

The Recombinant relationships from experiments 1-3 are:

Exp. 1 T-A = 12 map units Exp. 2 A-S = 5 map units Exp. 3 T-S = 18 map units

An arrangement that fits the data would be:

BACK

 

Carbon Dioxide Use in Plants

 

 

Do Plants Consume or Release CO2?

 

Introduction

The rate of photosynthesis can be determined by measuring the rate of production of sugar or oxygen or by measuring the rate of decrease in carbon dioxide concentration. A common aquarium plant called, Elodea,  can be used to show fast carbon dioxide is being removed from the water in which the Elodea is submerged.

6CO2 + 12H2O + light energy —> C6H12O6 + 6O2 + 6H2O

 

In this lab, you will use phenol red as an indicator to show whether CO2 is being consumed or produced in a reaction. It is well known that in the presence of light, plants perform photosynthesis. At the same time, plants are also performing cell respiration. To demonstrate this, we will determine whether CO2 is consumed or produced as Elodea is placed in either a light or dark environment. The change in CO2 will be detected by the pH indicator phenol red. Phenol red is yellow under acidic conditions (high H+ ion concentration), pink to magenta under basic or alkaline conditions (low H+ ion concentration), and orange under neutral conditions. A change in the amount of CO2 will cause a directly proportional change in H+ ion.

If the CO2 concentration decreases, the H+ ion concentration will also decrease, and the solution will change to pink, becoming basic.

If the CO2 concentration increases, the H+ ion concentration will also increase, and the solution will change to yellow, becoming acidic.

Neutral solutions of phenol red will be orange.

Materials:

phenol red solution,  4 sprigs of Elodea, soda straw, 4 test tubes, labeling marker, 100 ml graduated cylinder, beaker, aluminum foil

Procedure:

  1. Create a solution of phenol red by adding concentrated phenol red to about 100 ml of water in a beaker. The phenol red may change color as a result of adding water (depending on how acidic your tap water is). Your goal is to make your solution a neutral orange color. You can do this by gently blowing into the solution with a straw.
  2. Label 4 test tubes 1, 2, 3, and 4.
  3. Once you have the solution at an orange color, transfer it to 4 test tubes (they should be filled about 2/3 full with your orange solution).
  4. Place a cut piece of Elodea (cut end up) into tubes 2 and 4 and tightly cap.
  5. Test tubes 3 and 4 will not have Elodea. Cap and then cover these tubes with aluminum foil so no light can enter.
  6. Place tubes 1 and 2 in bright light.
  7. Place tubes 2 and 4 in the dark.
  8. After 24 hours, uncover and examine all 4 test tubes and record the results.

Data:

 

Test Tube # Contents of Tube Initial Color Final Color
1
2
3
4

 

Conclusion:

1. What test tubes served as the controls in this experiment. Why?

 

 

2. What was the dependent variable?

 

3. Do you think there would have been any change in any of the test tubes if they were left for 48 or 72 hours? Explain.

 

 

4. Describe and explain what happened in the test tubes.

 

 

 

5. Why did the color change occur?

 

6. Where does the carbon dioxide that is removed from the solution go?

 

7. What other process goes on in plant cells that requires oxygen and produces carbon dioxide?

 

8. What was the purpose in tightly capping all four test tubes?

 

 

Campbell Problem 10

Molecular Genetics Problem 10
10. An aneuploid person is obviously female, but her cells have two Barr bodies. What is the probable complement of sex chromosomes in this individual?

This individual probably is XXX.

The individual is a female. Nondisjunction of sex chromosomes produces a variety of aneuploid conditions in humans. Most of these conditions appear to upset genetic balance less than aneuploid conditions involving autosomes. Extra copies of the X chromosome are deactivated as Barr bodies in the somatic cells. Females with trisomy of the X chromosome (XXX), which occurs about once in approximately 1000 live births, are healthy and cannot be distinguished from XX females except by karyotype.

An Example of nondisjunction:

Klinefelter’s syndrome

49 ,XXXXY

This karyotype shows a variant of Klinefelter’s syndrome.

Individuals with this syndrome are male, typically with the karyotype 47,XXY.

Individuals with Klinefelter’s syndrome exhibit a characteristic phenotype including tall stature, infertility, gynecomastia and hypogonadism.

Aneuploidy above one extra chromosome is usually fatal but because of X-inactivation, which “turns off” all but one X chromosome per cell, the effects of 3 extra chromosomes are reduced.

BACK 

Cell Division Worksheet Ch 8 BI

 

Cell Division

 

 

Section 8-1 Chromosomes

 

1. What molecule in cells stores the genetic information?

2. DNA is built of billions of subunits called __________________________.

3. What are chromosomes, & when can they be seen?

4. What is the shape of chromosomes, & what 2 things are they made of?

5. Each chromosome is a single ________________ molecule with ____________________.

6. What is the purpose of histones?

7. What is the purpose of nonhistone proteins?

8. Chromosomes consist of two identical _____________________ called _________________.

9. What is a centromere?

10. Draw and label the parts of a chromosome.

11. Chromatids _______________________ during cell division so the two new cells will each
receive _________________ chromatid.

12. How does DNA appear in a cell between cell divisions?

13. What is chromatin & when does it exist in cells?

14. How many chromosomes do prokaryotes have?

15. How many chromosomes do each of these organisms have:
a. humans?
b. dog?
c. fruit fly?

16. What are the two categories of chromosomes?

17. Give 2 functions of the sex chromosomes.

18. Name the 2 sex chromosomes & tell what combination determines a male and a female organism.

19. What are autosomes & how many autosomes are in human cells?

20. What are homologous chromosomes & how do they compare to each other?

21. What is a karyotype?

22. In a human karyotype, where would each of these be found:
a. sex chromosomes?
b. longest homologs?
c. autosomes?

23. What is the main difference between diploid & haploid cells?

24. What type of cells in human would be diploid? Haploid?

25. How are diploid cells abbreviated? Haploid cells?

26. When a haploid (1n) ______________________ cell combines with a haploid
_______________________ cell, the new cell will be ______________________
or ____________________________.

 

Section 8-2 Cell Division

 

27. All cells are derived from ___________________________________.

28. What is cell division?

29. Define binary fission.

30. Describe the stages in binary fission of a prokaryote.

31. How do the two new cells compare to each other after binary fission? How do they compare to the original cell?

32. What two main cellular parts must be divided in eukaryotic cell division?

33. Name the 2 types of cell division in eukaryotes.

34. Define mitosis.

35. What type of cell uses mitosis?

36. What effect does meiosis have on the chromosome number of a cell?

37. How do the cells produced by meiosis reestablish a complete set of chromosomes?

38. What is the cell cycle?

39. Draw & label all parts of the cell cycle. (Figure 8-5, page 149)

40. What is the time between divisions in the life of a cell called?

41. How many phases is interphase divided into? Cell division?

42. Name the 2 parts of cell division.

43. What happens to the cell in each of these two parts of cell division?

44. In what stage do cells spend most of their time?

45. What is the size of cells immediately following cell division?

46. Name the 1st stage of interphase & tell what happens to the cell.

47. What stage of interphase do cells enter once they become mature?

48. What happens to a cell during the S phase of interphase?

49. What is the last stage of interphase called & what is happening to the cell?

50. What is the Go phase and what type of human cells are in this phase?

51. Name the 4 stages of mitosis in order.

52. What cellular part actually divides during mitosis?

53. Describe everything that happens to a cell during prophase.

54. Sketch and label a picture of a cell in prophase.

55. What are centrosomes & when do they appear?

56. What type of cell has centrosomes?

57. What is found inside centrosomes?

58. Are centrioles found in both plant & animal cells? Explain.

59. What forms from centrioles & what is their function?

60. Name the 2 types of fibers that make up the mitotic spindle & describe each one.

61. Describe everything that happens to a cell during metaphase.

62. Sketch & label a cell during metaphase.

63. Describe everything that happens to a cell during anaphase.

64. Sketch and label a cell during anaphase.

65. Describe everything that happens to a cell during telophase.

66. Sketch & label an animal cell during telophase.

67. Mitosis is division of the _______________________, while ________________________
is the division of the cytoplasm.

68. Define cytokinesis.

69. Describe how cytokinesis occurs in animal cells & include a drawing (figure 8-7, page 151)

70. The ____________________ pinches a dividing animal cell into two new cells by the action
of ______________________________.

71. How does the cell plate from during cytokinesis of a plant cell?

72. Sketch and label a plant cell during cytokinesis (figure 8-8, page 151)

73. How do the new cells formed after mitosis & cytokinesis compare in size & chromosome number to each other and the original cell that divided?

74. The original cell that divides is called the _______________________ cell, while the
two new cells are called ____________________ cells. (from lecture)

 

Section 8-3 Meiosis

 

75. What is meiosis?

76. What type of cell undergoes meiosis?

77. Meiosis produces ___________________ reproductive cells called ____________________.

78. Name the 2 human gametes & tell their chromosome number.

79. What is the chromosome number for humans?

80. The fusion of a _________________ and an _____________ produces a
________________ with 46 (2n) chromosome number.

81. Cells starting mitosis & meiosis begin with a ____________________ set of chromosomes.

82. How many times do cells divide during meiosis?

83. What are the stages of meiosis called?

84. Explain what happens during Meiosis I to each of these structures:
a. chromosomes?
b. spindle?
c. nucleus?
d. nucleolus?

85. What is synapsis & when does it occur?

86. What is a tetrad?

87. How are genes aligned on homologous chromosomes?

88. Explain what happens during crossing-over?

89. Sketch and color a picture of chromosomes during crossing-over. (Figure 8-10, page 154)

90. What type of material is exchanged during crossing-over?

91. Crossing over results in genetic _______________________________.

92. Draw a cell during anaphase I and explain what is occurring.

93. What is independent assortment & what result does it produce?

94. Name 2 things that occur during telophase I.

95. How many cells are formed at the end of Meiosis I & how many copies of chromosomes does each cell have?

96. Is DNA copied before Meiosis II?

97. How many cells form at the end of Meiosis II and how many chromosomes do they contain?

98. In humans, meiosis occurs in the ___________________ and in the __________________
producing cells called ________________________.

99. Define spermatogenesis & tell where it occurs.

100. Sketch spermatogenesis (Figure 8-12a, page 155).

101. What are spermatids & how many form from meiosis?

102. Define oogenesis & tell where it occurs.

103. Sketch oogenesis (Figure 8-12B, Page 155).

104. Mature egg cells are called ___________________.

105. Explain how only one egg cell is formed instead of four from meiosis.

106. What are the 3 other products of meiosis called?

107. Define asexual reproduction.

108. Name 2 types of asexual reproduction.

109. Name a type of asexual reproduction in unicellular organisms.

110. How do the offspring of asexual reproduction compare to their parents?

111. Define sexual reproduction.

112. How do offspring from sexual reproduction compare to their parents?

113. Is there ever a case in sexual reproduction where offspring can be genetically alike? Explain.

114. What is the evolutionary advantage of sexual reproduction?

BACK