Amphibian

 

Amphibians   All Materials © Cmassengale  

 

Amphibian Evolution:

Arose from lobe-fined ancestor called Crossopterygians
Land plants & insects provided new food source
Had primitive lungs & short, limb like fins for short periods on land
Appeared during late Devonian
Icthyostega early amphibian with 4 limbs, lungs, & a tail for swimming

Adaptations:

Four limbs with claws on digits (toes)
Lungs instead of gills
Both internal & external nares (nostrils)
Three chambered heart (two atria & one ventricle)
Double loop blood circulation to lungs & rest of body cells

Skin with keratin (protein) to prevent water loss
Necks to more easily see & feed
Most with smooth, moist skin to take in dissolved oxygen
Some with oral glands to moisten food they eat
Webbed toes without claws
Ectothermic – body temperature changes with environment
Show dormancy or torpor (state of inactivity during unfavorable environmental conditions)
Hibernate in winter and aestivate in summer
Aquatic larva called tadpole goes through metamorphosis to adult
Metamorphosis controlled by hormone called thyroxine

American Toad Tadpole photograph
Tadpole

External fertilization with amplexus (male clasps back of female as sperm & eggs deposited into water)
Eggs coated with sticky, jelly like material so they attach to objects in water & do not float away
Eggs hatch into tadpoles in about 12 days


Eggs

  • Males with vocal sacs to croak
  • Digested system adapted to swallow prey whole
  • Well developed muscular system

Classification:

  • Anura – frogs & toads
  • Urodela – salamanders & newts
  • Apoda – caecilians
  • Trachystoma – sirens or mud eels

Anuran Characteristics:

  • Both terrestrial & freshwater species
  • Tadpole with tail, gills, & two-chambered heart
  • Adults without a tail, four limbs, & lungs
  • Frog skin smooth & moist for cutaneous respiration, while toads is rough & warty (poison glands)

 


Frog

Toad

 

  • Long hind limbs for jumping
  • Long, forked tongue hinged at front of mouth

Urodela Characteristics:

  • Includes salamanders & newts
  • Have elongated bodies with a tail & four limbs 
  • Smooth, moist skin for cutaneous respiration
  • Less able to stay on dry land than anurans

Spotted salamander photograph
Spotted Salamander

  • Size from a few centimeters long to 1.5 meters
  • Nocturnal when live in drier areas
  • Newts are aquatic species

red-spotted newt photograph
Red Spotted Newt

  • Lay eggs in water or damp soil
  • Some bear live young
  • May or may not go through tadpole stage (some hatch & look like small adult)

Apodan Characteristics:

  • Includes caecilians
  • Tropical, burrowing, worm like amphibians
  • Legless
  • Small eyes & often blind
  • Eat worms & other invertebrates
  • Average length 30 centimeters, but can grow up to 1.3 meters
  • internal fertilization
  • Female bear live young


Caecilian

Trachystoma Characteristics:

  • Includes mud eels or sirens
  • Known as “rough mouth” amphibians
  • Found in eastern U.S. & southern Europe
  • Have minute forelimbs & no hindlimbs


Mud Eel or Siren

External Frog Anatomy:

  • Live double life on land & water
  • Powerful hind legs for jumping & swimming fold under body when at rest
  • Bulging eyes to stay submerged but still see predators
  • Blinking eyelids protect eyes from dust & dehydration
  • Nictitating membranes clear to moisten eye & see underwater
  • Internal nostrils or nares allow frog to breathe underwater
  • Tympanic membranes or eardrums behind each eye transmit sound through bone called columella to inner ear
  • Eustachian tubes connect mouth & middle ear to equalize pressure

  • Males croak or make sound to attract females & ward off other males
  • Have protective coloration from cells called chromatophores
  • Granular glands secrete foul tasting or poisonous substance
  • Mucus glands lubricate skin for oxygen to be dissolved & absorbed

Internal Frog Anatomy:
Skeletal System

  • Nine spinal vertebrae (1 cervical in neck, 7 trunk, & 1 sacral supporting hind legs)
  • Urostyle long, slim bone connecting sacral vertebrae & trunk
  • No rib cage, but pectoral girdle forms shoulders & connects front legs
  • Pelvic girdle connects to hind legs

Digestive System

  • Tongue sticky, forked, & hinged at front of mouth so can be extended out to catch insects
  • Can pull eyes inward to help swallow food
  • Two, sharp, backward-pointing  vomerine teeth in roof of mouth help prevent prey from escaping
  • Maxillary teeth line the edge of the upper jaw
  • Alimentary canal (mouth, esophagus, stomach, small & large intestines, and cloaca) is where food is digested, absorbed & wastes eliminated
  • Stomach makes gastric juices to break down food
  • Pyloric sphincter muscle controls movement of food from stomach into first part of small intestine called duodenum
  • Liver makes bile to digest fats; stored in gall bladder
  • Pancreas makes pancreatic juice to digest food in small intestine
  • Ileum is coiled mid portion of small intestine
  • Mesentery is a fanlike membrane holding the intestine in place
  • Wastes collect in large intestine & then move into cloaca along with eggs, sperm, & urine until they leave body through the anus

 

 

Circulatory System

  • Need more oxygen to burn increased amount of food needed to live on land
  • 3 chambered heart (right atrium receives deoxygenated blood from body, left atrium receives oxygenated blood from lungs, & ventricle pumps blood to lungs & rest of the body)
  • Double loop blood circulation (pulmonary from heart to lungs & systemic from heart to rest of body)
  • Conus arteriosus carries blood from ventricle to body cells

Respiratory System

  • Tadpoles use gills to breathe
  • Adult frogs breathe through lungs & moist skin (cutaneous respiration)
  • Glottis is the opening into throat & lungs

Excretory System

  • Carbon dioxide excreted through skin & lungs
  • Kidneys filter blood & store urine in urinary bladder until leaves cloaca

Nervous System

  • Olfactory lobes at base of brain detect smells
  • Cerebrum behind olfactory lobes controls muscles
  • Optic lobes detect sight
  • Cerebellum controls balance & coordination
  • Medulla oblongata controls heart rate & breathing
  • Cranial nerves connect brain & spinal cord, while spinal nerves branch off the spinal cord to muscles & sensory receptors
BACK

 

Antibiotic Resistant Lab

Antibiotic resistance of bacteria

Procedure Using a sterile loop, pick an isolated colony from you bacterial plate. Try to find one that grew well but is all by itself. Move the colony (don’t scoop up the agar) to a new plate. Using a moist, sterile, cotton swab, spread the bacteria around on the plate.

The goal is to get an complete, even, coverage of bacterial growth on the plate (called a “lawn”). Remember to open the plate only minimally, using the lid as an “umbrella” to prevent contamination (see image below). Label each plate on the bottom (agar contaning side) and store it for examination during next week’s lab.Each new prepared plate will receive four paper discs containing antibiotics. We will be using several different types of Antibiotics and/or antimicrobials.

(please fill in which antibiotics you used below)

  • 1
  • 2
  • 3
  • 4

The antibiotic discs come in a little tube-like dispenser. To remove the discs take a sterile toothpick and push out a disc into your plate. Use the toothpick to gently press the disc onto the agar. Once you have added the five antibiotic discs to your plates, make sure the plates are labelled and store them in the back of the lab until next week.

If the bacteria are susceptible to the antibiotic a zone of inhibited growth will be evident next week. Measuring the size of this zone is a relative indication of the effect of the antibiotic on the particular bacteria.

Bacteria possess several characteristics that enable them to become resistant to antimicrobial drugs:

  • Asexual reproduction
  • Short generation times
  • High mutation rates

Some Information on Antibiotics

Questions

1. Name two ways (1. and 2. ) that common human practices towards antimicrobials aids bacteria in becoming resistant.

 

2. Name two reasons your Physician will perform cultures such as the ones you have done in this lab.

 

 

4. How are materials are collected for cultures?

 

 

5. Why is neccesary to use sterile technique when obtaining cultures?

 

Amphibian Study Guide BI

Amphibian Study Guide

Describe what happens to a tadpole during its metamorphosis.
Explain why amphibians must return to the water to reproduce.
What characteristics of the frog’s skeleton make it adapted for jumping.
What is a series of body changes during an amphibian’s life cycle called?
Does a frog’s heart pump oxygenated or deoxygenated blood? Explain.
Are adult frogs omnivores, herbivores, or carnivores? tadpoles?
Tell some unusual ways some frogs undergo their metamorphosis.
Where  must frogs lay their eggs?
Give the order for amphibians without tails.
Where do toads lay their eggs?
What does the word “amphibian” mean?
What is the earliest known amphibian fossil?
Where does fertilization take place in most amphibians?
Describe the skin of amphibians. How does this compare to reptiles?
Describe caecilians.
What is the purpose of the cloaca in amphibians?
Amphibians with slender bodies and no limbs are _____________.
Why do amphibians need thin, moist skin?
Name the 2 ways gases are exchanged in amphibian adults.
Give the order for newts & salamanders.
BACK

 

Amino Acids

Listed below are the common names and structural formulas of the twenty amino acids found in proteins. They are arranged alphabetically.

Structure of alanine. [str5ala.jpg]
alanine
Structure of arginine. [str5arg.jpg]
arginine
Structure of asparagine. [str5asn.jpg]
asparagine
Structure of aspartic acid. [str5asp.jpg]
aspartic acid
Structure of cysteine. [str5cys.jpg]
cysteine
Structure of glutamic acid. [str5glu.jpg]
glutamic acid
Structure of glutamine. [str5gln.jpg]
glutamine
Structure of glycine. [str5gly.jpg]
glycine
Structure of histidine. [str5his.jpg]
histidine
Structure of isoleucine. [str5ile.jpg]
isoleucine
Structure of leucine. [str5leu.jpg]
leucine
Structure of lysine. [str5lys.jpg]
lysine
Structure of methionine. [str5met.jpg]
methionine
Structure of phenylalanine. [str5phe.jpg]
phenylalanine
Structure of proline. [str5pro.jpg]
proline
Structure of serine. [str5ser.jpg]
serine
Structure of threonine. [str5thr.jpg]
threonine
Structure of tryptophan. [str5trp.jpg]
tryptophan
Structure of tyrosine. [str5tyr.jpg]
tyrosine
Structure of valine. [str5val.jpg]
valine