Biochemistry Bi Worksheet

 

Biochemistry Worksheet

 

Section 3.1 – Properties of Water  

 

1. Why is water such an important molecule to living things?

 

2. Describe the chemical make up and type of bonding found in water molecules.

 

3. Explain why the hydrogen and oxygen atoms don’t share electrons equally in a water molecule.

 

4. What is the effect of this uneven sharing of electrons in water?

 

5. Sketch a molecule of water showing the charges on the molecule.

 

 

6. What is the overall charge on a water molecule? Explain why.

 

 

7. Define polar compound and give an example.

 

8. Water’s polarity makes it very effective in _____________ other substances.

9. Name 2 types of compounds that dissolve well in water.

10. What happens when an ionic compound such as sodium chloride (table salt) dissolves in water?

 

11. Water molecules are ___________ to other water molecules.

12. What type of bonding holds 2 or more water molecules together?

13. Are hydrogen bonds strong or weak bonds? Can they be easily broken?

14. Water molecules attracting other water molecules is called _________________.

15. Cohesion of water molecules produces ________________ tension making water seem like it has a “skin” on it. Surface tension enables some _____________ to walk across the surface of the water.

16. Water molecules attracting other types of molecules is called _________________.

17. Adhesion and cohesion together enable water molecules to move ____________ through narrow tubes against the force of gravity.

18. The above property of water is called _________________.

19. Give an example of an organism using capillarity.

 

20. What must be true for water to change temperature?

21. What effect does heating water have on the hydrogen bonds holding the water molecules together? What happens to the speed at which the molecules are moving?

 

22. Give an example of how this water property helps organisms in the environment.

 

Section 3.2 – Carbon Compounds

23.  What is an organic compound?

 

24. Besides carbon, name 3 other elements that make up most organic compounds.

25. Carbon dioxide, CO2, is NOT an organic compound. Explain why.

 

26. How many electrons are in the outermost energy level of carbon? How many does it need to have this energy level filled?

27. How many covalent bonds can carbon form?

28. Name 3 structural shapes that form whenever carbon atoms bond to other carbon atoms.

 

29. How many electrons are being shared in a single covalent bond? double covalent bond? triple covalent bond? quadruple covalent bond?

 

30. Draw these 3 molecules and circle a single bond, double bond, and a triple bond in your drawings — benzene, acetylene, and ethanol.

 

 

31. Explain what is meant by a functional group, & tell what effect they have on the molecules they are attached to.

 

32. Write the formula for these functional groups (use your textbook & handout) — hydroxyl, carboxyl, phosphate group, amino group, and methyl group.

 

 

33. Hydroxyl groups attached to carbon atoms forms an _____________. Name an alcohol used in humans to assemble molecules needed for life.

34. Large carbon molecules are built from smaller, simpler molecules called ____________.

35. Large carbon molecules made of monomers are called _______________.

36. What are large polymers called?

37. What type of reaction links monomers to make polymers?

38. Sketch a molecule of sucrose (table sugar) formed from condensation. Name the 2 sugars that were combined to form sucrose.

 

 

39. Condensation reactions involve the removal of a molecule of ____________.

40. What reaction is used to breakdown polymers? Is water added or removed? How does this compare to condensation?

 

41. All life processes require a constant supply of ____________. Name the molecule used by cells to get energy. Give its abbreviation.

42. ATP contains what 3 functional groups covalently bonded together? Write the formula for this functional group.

43. Which bonded phosphate group on ATP releases the MOST energy when broken?

Section 3.3 – Macromolecules

44. Name the 4 main classes of macromolecules (organic molecules) & tell what 3 elements all of these contain.

 

Carbohydrates store energy for organisms!

45. In what ratio are hydrogen & oxygen atoms in carbohydrates?

46. In what 3 forms do carbohydrates exist?

47. What are the monomers of carbohydrates called? What is their common name? Give the ratio of carbons, hydrogens, & oxygens.

48. Name the 3 MOST common monosaccharides.

49. Sketch & label a molecule of each of these monosaccharides. How do they compare? Write the chemical formula for all three.

 

 

 

50. Because all 3 simple sugars have the same chemical, but different structural formulas, they are called _______________.

51. What are double sugars called? Name & describe the process that forms them.

 

52. Name a disaccharide.

53. What forms a polysaccharide? Name a polysaccharide found in animals. Name 2 found in plants?

 

54. What chemical reaction formed these large molecule? What reaction would be needed to break these molecules?

 

Proteins are used to build cells, & they act as enzymes!

55. What are the 4 main elements making up proteins? How many covalent bonds does each of these elements form?

 

56. Sketch these two amino acids — glycine & alanine.  Circle the center carbon, place a triangle around the amino group, and put a box around the carboxyl group.

 

 

 

 

 

57. What are the monomers of proteins called? How many are there? Name the 4 things bonded to the center carbon of this monomer.

 

 

58. The main difference among amino acids is their ___________ group. What is the R-group on glycine? on alanine?

59. Differences in R-groups give different proteins different ______________.

60. How does a dipeptide form? Sketch the dipeptide formed from glycine and alanine. What molecule had to be removed to join these 2 amino acids?

 

 

 

61. What do you call the covalent bonds that hold amino acids together? Put a box around these bond in the sketch you did on question 60.

62. Long chains of amino acids are called ___________________ and these join together to make a ________________.

63. Hydrogen bonding among individual amino acids in a chain cause what effect on the protein’s shape?

64, What is the effect of temperature on protein shape? Give an example of this.

 

65. Most proteins act as catalysts or __________________ inside of cells.

66. The substance an enzyme is acting upon is called the _____________ and it must ______ into a place called the active site on the enzyme.

67. When a substrate joins with an enzyme, what effect does this have on chemical bonding of that substrate? Is the enzyme affected temporarily or permanently? How is it affected?

 

68. When chemical bonds in a substrate are weakened, what effect does this have on activation energy needed to start the reaction?

69. After the reaction, what happens to the products? Can the enzyme be re-used & why?

 

70. Besides temperature, what else can effect how an enzyme works by changing the enzyme’s shape? Can the reaction still take place?

Lipids include fats that are used for long-term energy storage!

71. Are lipids polar or nonpolar? What happens to lipids when they are placed in water?

72. Compared to carbohydrates, what is true about the ratio of carbon & hydrogen atoms to oxygen atoms? If a compound has more bonds, what can it store more of in those bonds?

 

73. Most lipids are made of ______________ acids. Describe their shape. What functional group is found on the head end of the molecule?

 

74. Sketch these 2 fatty acids — palmitic & linoleic.  Circle the carboxyl group on the “head” of the molecule. Is this end polar or nonpolar? Will this end be attracted to  or repelled by water?

 

 

 

 

 

75. Are both ends of a fatty acid polar? Explain.

76. Hydophilic means water ___________. Which end of  a fatty acid is hydrophilic. The nonpolar end of a fatty acid is said to be _______________ or “water fearing”.

77. Which end of a fatty acid chain WOULD dissolve in water? Which WOULDN’T?

 

78. In what type of fatty acid are there only single bonds in the carbon chain? Name one such fatty acid.

79. What type of bond appears in an unsaturated fatty acid? Give an example of an unsaturated fatty acid. Go back to your fatty acid drawings in question 74 and put a box around the double bond in the unsaturated fatty acid.

 

80. Name the 3 groups of complex lipids.

81. What makes up a triglyceride? What is the difference between a saturated & unsaturated triglyceride?

 

82. What type of triglycerides tend to be solids at room temperature & why? Which are liquids & why?

 

83. What type of triglyceride would this of  substance be — vegetable oil?  butter & shortening?

84. What makes up a phospholipid? How are they different from triglycerides? What main part of a cell is made of phospholipids?

 

85. What is meant by a lipid bilayer? What makes this such an effective barrier between the inside & the outside of the cell?

 

86. Wax is another complex lipid. Describe its structure.

 

87. Waxes are highly _________________. Explain how plants make use of this property? animals?

 

88. What makes up steroids? To what group of organic compounds do steroids belong? How are they used in animals?

 

89. Name a steroid made by the body & used by nerve cells.

Nucleic acids  store genetic information for cells!

90. Give the name & abbreviation for 2 nucleic acids found in cells.

 

91. DNA and RNA are both examples of _____________ made of linked monomers called ________________.  The instructions in these molecules is used to make ____________.

92. Name the 3 parts to a nucleotide then draw and label one.

 

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Biochemistry Quiz

Name: 

Biochemistry Quiz

 

True/False
Indicate whether the sentence or statement is true or false.
1.
Because water is a polar molecule, it tends to cause ionic compounds mixed in water to dissociate into ions.
2.
Capillarity is apparent when you put a straw in water and the water level inside the straw rises higher than the level in the surrounding container.
3.
Organic compounds are substances produced and found in living things.
4.
Functional groups are side groups of carbon compounds that confer specific properties to these compounds.
 

Multiple Choice
Identify the letter of the choice that best completes the statement or answers the question.
5.
Which of the following characteristics of water is not a result of hydrogen bonding?
a.
adhesive strength
b.
capillarity
c.
cohesive strength
d.
All of the above are a result of hydrogen bonding.
6.
Carbon is different from most other elements in that
a.
it has four electrons in its outermost energy level.
b.
it readily bonds with other carbon atoms.
c.
it can form single, double, or triple bonds with other atoms.
d.
it shares two electrons with another atom when it forms a covalent bond.
7.
Which of the following is not true of alcohols?
a.
They contain a hydroxyl group (–OH).
b.
They are polar molecules.
c.
They can affect processes in living things, either positively or negatively.
d.
They are the only kind of functional group in organic molecules that contain oxygen.
8.
Amino acids are monomers of
a.
disaccharides.
c.
nucleotides.
b.
proteins.
d.
steroids.
9.
Which organic molecule below is most closely related to lipids?
a.
amino acids
c.
nucleotides
b.
CH2 chains
d.
sugars
10.
Which organic molecule below is most closely related to nucleic acids?
a.
amino acids
c.
nucleotides
b.
CH2 chains
d.
sugars
 

Completion
Complete each sentence or statement.
11.
Water is very effective at dissolving other polar substances because of its ____________________.

12.
Breaking of ____________________ bonds is the first thing that happens when water is heated, which means that it takes a great deal of thermal energy to raise the temperature of water.

13.
In the molecule that has the chemical formula C2H4, the carbon atoms are bonded together with a ____________________ bond.

14.
Because oxygen atoms tend to attract positively charged atoms, organic compounds that contain oxygen atoms tend to form ____________________ bonds.

15.
In a condensation reaction, two molecules become linked together and a molecule of  ____________________ is produced.

16.
The formation of polymers from monomers occurs as a result of ____________________ reactions, and the breakdown of polymers into monomers occurs as a result of ____________________ reactions.

17.
Lipids are ____________________ molecules because they have no negative and positive poles.

18.
A phospholipid is a molecule with a(n) ____________________ head.

19.
Lipids are ____________________ molecules because they have no negative and positive poles.

20.
A phospholipid is a molecule with a(n) ____________________ head.

 

Check Your Work     Reset

Bacteria Culturing Activity

 

Where are Bacteria Found?  

 

 

Introduction:

They’re everywhere. Bacteria are the huddled masses of the microbial world, performing tasks that include everything from causing disease to fixing nitrogen in the soil. The estimated number of bacteria on Earth is five million trillion trillion — that’s a five with 30 zeroes after it.  When people think of bacteria, they likely first consider the nasty ones that cause disease, but the bacteria inside all animals combined — including humans — makes up less than one percent of the total amount. By far the greatest numbers are in the subsurface, soil and oceans.

 

Objectives:

  1. To take bacterial swabs from various places in the school
  2. To inoculate a petri dish with a bacterial culture
  3. To count bacterial colonies
  4. To determine what kind of environmental conditions influence bacterial growth

Materials: 

Petri dish,  pencil,  incubator, hot water bath, nutrient agar, thermometer

Procedure (Part A): Petri Dish Preparation

  1. Set up a hot water bath at 95oC.
  2. Loosen the caps and place nutrient agar bottle in hot water bath until agar liquefies. (Agar melts above 95oC and remains liquid until cooled to about 45oC.)
  3. Remove agar bottles and allow the agar to cool to about 50-55oC.
  4. Partially lift the cover of the petri dish and pour about 15-20ml of liquid to cover 2/3 of the plate surface.
  5. Lower the lid of the dish and gently swirl the plate to spread the media over all the bottom surface.

  1. Repeat step 5 to fill other petri dishes.
  2. DO NOT MOVE the covered plates until the nutrient agar has solidified.
  3. Once the plates are solidified, turn the plates upside down (presents condensation from getting on the agar surface).
  4. From this moment on, keep the plates upside down (condensation will disappear) in a dark, dust-free place in the room until ready to add bacteria. If plates will not be used for several days, refrigerate them.
  5. Check plates for contamination before proceeding to Part B. Discard contaminated plates.

Materials: 

Petri dish with nutrient agar, sterile cotton swabs, permanent marker, index card with sample location, pencil,  incubator

 

Procedure (Part B): Collecting Bacteria

  1. Choose an index card to determine your sample location
  2. Turn the petri dish upside down, and using your marker, place your initials, date and sample location along the bottom perimeter of the dish, NOT in the middle
  3. Get your sterile Q-tip, being very careful not to touch the side that will collect your sample. Go to your assigned area and quickly swab and return with your sample! (Sample locations included door handles, water faucets, desk tops, etc.)
  4. Carefully open your dish just enough to lightly rub your Q-tip in a zigzag pattern across the agar.

  1. Draw what your dish looks like in Figure 1 and record the number of bacterial colonies, if any, present on the agar surface in table 1
  2. Place your petri dish upside down in the incubator to be examined again in a few days.
  3. Recheck the plates after 1 day, 2 days, and 5 days. Count and record the number of bacterial colonies on each plate. If the plate is  completely covered with bacteria, record “lawn” in the data table.
  4.  Ignore “fuzzy” appearing colonies that are actually fungi!

Example of Bacterial Colonies on Plate

Data:

Reminder — Fuzzy Colonies = Fungus not Bacteria

Figure 1 

Day 1                Day 2                Day 5

   

Table 1:   Number of Colonies on petri dish 

    Location:
Day Number of Colonies

 

Analysis:  

  1. Compare the number of colonies on your plate on day 5 with the plates collected from other locations. Did any of the areas show a greater number of bacteria? How many clusters of bacteria appear to be growing in each petri dish?
  2. Which petri dish had the most growth? The Least?
  3. Why was the agar sterilized before this investigation?
  4. What kind of environmental conditions seem to influence where bacteria are found?
  5. How can you control the amount of bacteria that you will encounter?
  6. Check the plate that the teacher has had open, exposed to the air for several days. What did you observe and why?

Dispose of the petri dishes carefully!  Place them in a biohazard bag to be autoclaved.

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Bacteria PPT Questions

Bacteria
ppt Q’s

Prokaryote & Eukaryote Evolution

1. What does our current evidence tell us about the evolution of prokaryotes and eukaryotes?

 

2. About how long ago did eukaryotes evolve from prokaryotes?

3. Name the 2 theories of cellular evolution.

 

4. Explain the infolding theory.

 

 

5. What does endosymbiosis mean?

 

6. Explain the endosymbiotic theory of cell organelle formation.

 

 

 

7. Name 2 organelles thought to have arisen in this way.

 

Prokaryotic & Eukaryotic Cells

8. Label the parts of this prokaryotic cell.

9. Name several structures that are found in eukaryotic, but NOT prokaryotic cells.

 

 

10. What type of cells are the most numerous on Earth?

11. What are the most common type of prokaryotic cells?

12. How old are the earliest prokaryotic fossils?

Classification of Life

13. Name the 3 domains and the organisms found in each.

     a.

     b.

     c.

14. ______________ are found in harsh environments.

15. Give 3 examples of harsh environments in which Archaebacteria can be found.

 

16. What group is referred to as the true bacteria?

17. What photosynthetic member is in this group?

Characteristics of Bacteria 

18. What must be used to view prokaryotic cells?

19.What cell structures are lacking in prokaryotes?

20. Do bacteria have ribosomes like other types of cells?

21. Describe the genetic material of the bacteria.  be sure to tell where it is found.

 

22. What surrounds the cytoplasm of bacterial cells?

23.What surrounds the outside of all bacterial cells?

24. Cell walls of true bacteria contain ____________________.

25. Some bacteria have a sticky ____________ around the cell wall to attach to __________ or other bacteria.

26. Besides the circular chromosome, where else can DNA be found inside a bacterial cell?

27. What is the size of most bacterial cells?

28. Compare the size of bacteria to the tip of a pin.

 

29. ____________ of the bacterial cell membrane are called _______________.

30. What two cellular processes can take place in mesosomes?

 

31. At what pH do bacteria do best?

32. Most bacteria act as ________________. Why is this so important?

 

33. How can some bacterial be harmful?  Give an example.

 

34. name two other important uses for bacteria.

 

35. What does motile mean?

36. Motile bacteria may have one or more ______________ for movement.

37. Flagella attach to the bacteria by the ___________ ___________.

38. The basal body attaches to the cell through both the cell _________and the cell ___________.

39. What protein makes up bacterial flagella?

40. Tell how these types of bacteria differ from each other:

     a. Monotrichous

     b. Lophotrichous

     c. Amphitrichous

     d. Peritrichous

41. What type of bacteria is this?

42. What are bacterial pili?

 

43. How do pili compare to flagella in size?

44. Give three functions of pili.

     a.

     b.

     c.

 

Bacterial Shapes

45. Name and describe 5 shapes used to classify bacteria. 

     a.

     b.

     c.

     d.

     e.

46. What does each of these prefixes tell you about the bacteria’s shape:

     a. Diplo-

     b. Strepto-

     c. Staphylo-

47. Sketch the shape of these bacteria:

     a. Coccus

     b. Bacillus

     c. Spirillium

     d. Diplococcus

     e. Streptococcus

     f. Staphylococcus

     g. Diplobacillus

48. E. coli is classified as what shape bacteria?

Bacterial Kingdoms

49. How do the cell walls of Archaebacteria differ from the true bacteria?

50. How do the cell membranes differ?

51. Are the ribosomes the same?

52. Are the gene sequences the same?

53. Do Archaebacteria require oxygen?

54. How is there environment different from true bacteria?

 

55. What are they commonly called?

56. How many groups make up the ancient bacteria and name them?

 

57. Methanogens live in _____________ environments. What is lacking in this environment?

58. How do methanogens get their energy?

 

59. Name 3 environments in which methanogens are found.

 

 

60. How do methanogens help cows?

 

61. How did the methanogens get their name?

 

62. The __________ ___________ live in very salty environments.

63. How do they get their energy?

 

64. Name two bodies of water in which halophiles are found.

 

65. ______________ live in extremely hot environments.

66. Thermophiles that also live in acidic conditions are called _____________________.

67. Name 3 habitats in which thermophiles are found.

 

Kingdom Eubacteria

68.  Most true bacteria are ____________ and come in ________ basic shapes. Name the shapes.

 

69. Do eubacteria require oxygen?

70. How are they identified?

71. When was gram staining developed?

72. Describe Gram staining.

 

73. What colors do bacterial cell walls stain?

74. Describe the cell wall of Gram positive bacteria.

 

 

75. What color do they stain?

76. Can Gram positive bacteria be treated with antibiotics?

77.Name 5 Gram positive bacteria and tell how they’re used or what they may cause.

     a.

     b.

     c.

     d.

     e.

78. Describe the cell walls of Gram negative bacteria.

 

79. Are antibiotics effective against Gram negative bacteria?

80. Some photosynthetic Gram negative bacteria make ___________ instead of oxygen.

81. How do some Gram negative bacteria help plants?

82. Where can Rhizobacteria be found and what is their job?

 

83. _____________ are parasitic bacteria carried by ticks that may cause ___________ disease or _____________ _______________ _____________ fever.

84. Cyanobacteria are Gram ____________ and carry on ______________ to make food.

85. What is the common name for cyanobacteria?

86. What two main pigments do cyanobacteria contain?

87. What colors are cyanobacteria?

 

88. _______________ is a cyanobacterium that grows in chains.

89. Name the specialized structures on cyanobacteria that help fix nitrogen.

90. How do cyanobacteria cause eutrophication?

 

91. Spirochetes are Gram __________ bacteria that move by ___________.

92. Describe the motion of spirochetes.

93. Do all spirochetes need oxygen?

94. Spirochetes may be _______________, _______________, or symbiotic.

95. What are enteric bacteria? Give an example.

 

96. _______________ is an enteric bacterium that causes food poisoning.

97. How do chemoautotrophic bacteria get their energy?

 

Nutrition, Respiration, and Reproduction

98. Name and describe 4 modes of nutrition in bacteria.

     a.

     b.

     c.

     d.

99. Explain each of the following methods of respiration in bacteria.

     a. Obligate Aerobes-

 

     b. Obligate Anaerobes-

 

     c. Facultative Anaerobes-

 

100. Anaerobes carry on ______________ to release energy from food, while aerobes carry on ____________ _______________.

101. Bacteria reproduce asexually by what method?

102. Before the cell can divide, what must happen?

103. Is binary fission a slow or fast process?

104. How do the new cells compare with each other after binary fission? What are they called?

 

105. Bacteria can reproduce sexually by ________________.

106. Describe how conjugation occurs.

 

107. What is the function of pili in conjugation?

108. How do the new cells compare to each other after conjugation?

109. When can bacteria produce spores and why?

 

110. What are the spores called?

111. How long can an endospore survive?

112. Why are endospores such a problem in health care facilities and in the canning industry?

 

113. Bacteria can genetically change by _________________ and ____________________.

114. Disease-causing bacteria may become ______________ _____________ when they genetically change.

115. How do bacteria transform?

 

116. Describe transduction in bacteria and give an example of a product made by bacteria using this method.

 

Pathenogenic Bacteria

117. What are pathogens?

 

118. Pathogens may cause ____________.

119. What are toxins?

 

120. What is the difference between endotoxins and exotoxins?

 

121. Name a bacterium that produces each type of toxin.

     a. Endotoxin?

     b. Exotoxin?

 

 

AP Unit Cell Cycle Division

 

 

OPENERS:

Right Click on Topic & choose “SAVE AS” to Show any of these 5 minute class openers!

CELL CYCLE & MITOSIS

MEIOSIS

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NOTES:

 

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POWERPOINTS:

 

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WORKSHEETS & INTERACTIVES:

 

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LABS:

 

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TEST PREP:

 

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GREAT LINKS:

  • Cell Division: Binary Fission and Mitosis This site, from the University of Arizona, is an illustrated lecture place on mitosis and cell division. It contains many diagrams that may help you understand all the process of cell division.
  • Studying Cells  Introduce yourself to the cell as the fundamental unit of life and the scientific method.
  • The Cell Cycle & Mitosis  Understand the events that occur in the cell cycle and the process of mitosis that divides the duplicated genetic material creating two identical daughter cells.
  • Mitosis Animation Although the diagrams here are somewhat rough, they do a good job of showing the essential features of mitosis. Just remember that the figures show the nucleus, not the entire cell!
  • Meiosis Understand the events that occur in process of meiosis that takes place to produce our gametes.
  • Prokaryotes, Eukaryotes, & Viruses Learn about the cells that make up all living systems, their organelles, and the differences between living cells and viruses.
  • The Cytoskeleton Learn that the cytoskeleton acts both a muscle and a skeleton, and is responsible for cell movement, cytokinesis, and the organization of the organelles within the cell.
  • Online Onion Root Tips Estimate the amount of time cell spent in each mitotic phase in this animated cyber-version of the chapter’s lab investigation. After completing this activity, identifying the phases of mitosis will be a snap.
  • Spindle Microtubules These amazing pictures show microtubule organization at interphase and during several stages of mitosis. (The microtubules are stained green, and the DNA is stained blue.)
  • Cytokinesis Movie This site shows a very nice cytokinesis of a mouse cell growing in a dish.
  • Amphibian Embryology This site provides a good overview of how mitosis takes a fertilized egg and produces an animal from it

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