Chapter 16 – Molecular Basis of Inheritance

 

Chapter 16   Molecular Basis of Inheritance
Objectives
DNA as the Genetic Material
1. Explain why researchers originally thought protein was the genetic material.
2. Summarize the experiments performed by the following scientists that provided evidence that DNA is the genetic material:
a. Frederick Griffith
b. Oswald Avery, Maclyn McCarty, and Colin MacLeod
c. Alfred Hershey and Martha Chase
d. Erwin Chargaff
3. Explain how Watson and Crick deduced the structure of DNA and describe the evidence they used. Explain the significance of the research of Rosalind Franklin.
4. Describe the structure of DNA. Explain the base-pairing rule and describe its significance.
DNA Replication and Repair
5. Describe the semiconservative model of replication and the significance of the experiments of Matthew Meselson and Franklin Stahl.
6. Describe the process of DNA replication, including the role of the origins of replication and replication forks.
7. Explain the role of DNA polymerases in replication.
8. Explain what energy source drives the polymerization of DNA.
9. Define antiparallel and explain why continuous synthesis of both DNA strands is not possible.
10. Distinguish between the leading strand and the lagging strand.
11. Explain how the lagging strand is synthesized even though DNA polymerase can add nucleotides only to the 39 end. Describe the significance of Okazaki fragments.
12. Explain the roles of DNA ligase, primer, primase, helicase, topoisomerase, and single-strand binding proteins.
13. Explain why an analogy can be made comparing DNA replication to a locomotive made of DNA polymerase moving along a railroad track of DNA.
14. Explain the roles of DNA polymerase, mismatch repair enzymes, and nuclease in DNA proofreading and repair.
15. Describe the structure and function of telomeres.
16. Explain the possible significance of telomerase in germ cells and cancerous cell.

 

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Introduction Notes

Introduction

All Materials © Cmassengale

Study of Life  

  • First life forms arose on Earth more than 3.5 billion years ago  
  • Single-celled, microscopic organisms (living thing) appeared first & floated alone in seas  
  • Over 40 million species (types of organisms) exist with only about 2 million identified 
  • Many organisms are unidentified & new species are still discovered  
  • Biology is the study of all living things and how they interact with each other & their environment  
  • Over long periods of time, species changed or evolved so that new species arose from earlier organisms & came to inhabit almost every part of the earth (bacteria living in thermal vents, parasites living inside another organism, etc.)  
  • Organisms must adapt to their specific environment to survive & reproduce

 

Unifying Themes of Biology 

 The six unifying themes include:  

  1. Cell Structure & Function
  2. Stability & Homeostasis
  3. Reproduction & Inheritance
  4. Evolution
  5. Interdependence of Organisms
  6. Matter, Energy, & Organization

Cell Structure & Function  

  • Cell is the basic unit of structure & function  
  • All organisms are made of one or more cells; Unicellular (one celled) or Multicellular (Composed of more than one cell)  


UNICELLULAR AMOEBA

  • Cells are small but highly organized; they contain specialized structures that carry out the jobs of a cell called organelles  


CELLULAR ORGANELLES

  • There are many different kinds of cells, but all cells have similarities
  • All cells are surrounded by a cell membrane, contain cytoplasm, and have DNA (the genetic information for making new cells or cell structures)  
  • New cells made by unicellular organisms are identical (clones) to the parent cell that produced them – asexual reproduction  
  • Multicellular organisms begin life as one fertilized cell (sexual reproduction), but the cells multiplied and underwent differentiation (changed structure & function) to become many different kinds of cells  

Stability & Homeostasis  

  • All organisms maintain stable internal conditions such as body temperature & water content
  • Stable level of internal conditions called homeostasis  

Reproduction & Inheritance  

  • All organisms reproduce new organisms like themselves by transmitting hereditary material to their offspring  
  • DNA (Deoxyribonucleic acid) is a large molecule containing the hereditary material of the cell


DNA MODEL

  • In unicellular organisms like bacteria, DNA exists as a single loop or chromosome in the cytoplasm  


BACTERIA

  • In multicellular organisms, DNA is enclosed in a membrane known as the nucleus 
  • Genes are short segments of DNA the carry the instructions for a single trait of an organism  
  • DNA of a cell contains all of the genes (instructions) it will ever need  
  •  All body cells have a complete set of DNA (genome), but different types of cells use certain genes from the set; example: Muscle cells have the genes to make thyroxine, but they don’t use these genes  
  • In sexual reproduction, an egg (ovum) is fertilized by a sperm to form a zygote so the new organism is made of cells with hereditary information from both parents  
  • In asexual reproduction, cells copy their DNA & split so all new cells are identical  

Evolution  

  • Populations of organisms change over time or evolve (Theory of Evolution)  


DARWIN – THEORY OF EVOLUTION

  • Natural selection or “survival of the fittest” is the process that drives evolution  
  • Organisms with favorable traits are better able to survive & reproduce  
  • The survival of organisms with favorable traits causes a gradual change in populations of organisms over many generations  
  • Evolution by natural selection is driven by competition for resources such as food, habitat, mates  

Interdependence of Organisms  

  • Ecology is the study of the interaction of organisms with each other and their environment  
  • Sunlight is the ultimate energy for all organisms  
  • Energy from the sun is passed from one organism to another; producers (plants) to herbivore (plant eater) to carnivore (meat eater) to decomposers (break down dead organisms)  


ENERGY FLOW IN A FOOD CHAIN

  • Abiotic (nonliving factors) such as air, water, energy, soil, temperature, & minerals are also needed for survival  
  • Biotic factors include all living things on earth (plants, animals, fungi, microorganisms)  
  • Biosphere supports life & includes the biotic (all organisms) & the abiotic (all nonliving factors) on earth  
  • Organisms respond to their environment by:
    * Fleeing
    * Adapting
    * Dying
  • Most organisms can survive a temporary change, but permanent change can lead to extinction (dinosaurs)  
  • Thousands of species are listed endangered (population so small could become extinct)  
  • Human interference is the main cause for endangerment & extinction
    * Pollution of land, air, and water
    *Hunting for sport, food, and commercial products also threatens the survival of many organisms
    * Clear-cutting rain forests
    * Diverting rivers & lakes
    * Draining wetlands (everglades)
    *Global Warming  
  •   Endangered organisms can be protected & returned to larger population size (American Bison almost wiped out –60 million to 250 in 90 years- now several thousand herds)  


AMERICAN BISON

  • Species is a group of organisms so similar to one another that they can interbreed & produce fertile offspring  
  •  Extinction of any species upsets the balance of nature (Almost extinct Pacific Yew tree found to contain chemical used to treat cancer)  


PACIFIC YEW TREE

Matter, Energy, & Organization  

  • Organisms are highly organized, maintain internal order, & require a constant energy supply
  • Plants & unicellular organisms with chlorophyll capture sunlight through photosynthesis & store it in food to be used by other organisms  
  • Autotrophs or producers use sunlight, water, & carbon dioxide to make glucose (energy rich sugar) & oxygen – photosynthesis  

  • Heterotrophs (consumers) feed on producers or other consumers to get energy & release carbon dioxide  

   Biology affects life in many ways  

  • Biotechnology uses organisms to make products needed by people (human insulin made by bacteria)
  •   Fossils fuels (coal, oil, & natural gas) provide energy & materials such as nylon & polyester  


OIL DRILLING

  • Animal products such as wool, silk, and leather make clothing  
  •  Wood provides energy & shelter for us, but endangers other animals (spotted owl) when forests are cut  
  • New medicines, better water treatment & garbage disposal improves our health
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Chapter 17 AP Objectives

 

Chapter 17    From Gene to Protein
Objectives
The Connection Between Genes and Proteins
1. Explain why dwarf peas have shorter stems than tall varieties.
2. Explain the reasoning that led Archibald Garrod to first suggest that genes dictate phenotypes through enzymes.
3. Describe Beadle and Tatum’s experiments with Neurospora and explain the contribution they made to our understanding of how genes control metabolism.
4. Distinguish between the “one geneÐone enzyme” hypothesis and the “one geneÐone polypeptide” hypothesis and explain why the original hypothesis was changed.
5. Explain how RNA differs from DNA.
6. Briefly explain how information flows from gene to protein.
7. Distinguish between transcription and translation.
8. Compare where transcription and translation occur in prokaryotes and in eukaryotes.
9. Define codon and explain the relationship between the linear sequence of codons on mRNA and the linear sequence of amino acids in a polypeptide.
10. Explain the early techniques used to identify what amino acids are specified by the triplets UUU, AAA, GGG, and CCC.
11. Explain why polypeptides begin with methionine when they are synthesized.
12. Explain what it means to say that the genetic code is redundant and unambiguous.
13. Explain the significance of the reading frame during translation.
14. Explain the evolutionary significance of a nearly universal genetic code.
The Synthesis and Processing of RNA
15. Explain how RNA polymerase recognizes where transcription should begin. Describe the promoter, the terminator, and the transcription unit.
16. Explain the general process of transcription, including the three major steps of initiation, elongation, and termination.
17. Explain how RNA is modified after transcription in eukaryotic cells.
18. Define and explain the role of ribozyme.
19. Describe the functional and evolutionary significance of introns.
The Synthesis of Protein
20. Describe the structure and functions of tRNA.
21. Explain the significance of wobble.
22. Explain how tRNA is joined to the appropriate amino acid.
23. Describe the structure and functions of ribosomes.
24. Describe the process of translation (including initiation, elongation, and termination) and explain which enzymes, protein factors, and energy sources are needed for each stage.
25. Describe the significance of polyribosomes.
26. Explain what determines the primary structure of a protein and describe how a polypeptide must be modified before it becomes fully functional.
27. Describe what determines whether a ribosome will be free in the cytosol or attached to the rough endoplasmic reticulum.
28. Describe two properties of RNA that allow it to perform so many different functions.
29. Compare protein synthesis in prokaryotes and in eukaryotes.
30. Define point mutations. Distinguish between base-pair substitutions and base-pair insertions. Give examples of each and note the significance of such changes.
31. Describe several examples of mutagens and explain how they cause mutations.
32. Describe the historical evolution of the concept of a gene.

 

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BACK TO BIOLOGY I HOME PAGE CHAPTER 8

 

CHAPTER 8,  CELL REPRODUCTIONSECTION 8-1, CHROMOSOMES

DNA is a long thin molecule that stores Genetic Information.  The DNA in a human cell is estimated to consist of six billion pairs of nucleotides.

OBJECTIVES:  Describe the structure of a chromosome.  Compare prokaryotic chromosomes with eukaryotic chromosomes.  Explain the differences between sex chromosomes and autosomes.  Give examples of diploid and haploid cells.

CHROMOSOME STRUCTURE

1. During Cell Division, the DNA (CHROMATIN) in an Eukaryotic Cell’s Nucleus is coiled into very tight compact structures called CHROMOSOMES.(Figure 8-1)

2. Chromosomes are Rod Shaped structures made of DNA and Proteins.

3. The Chromosomes of stained Eukaryotic cells undergoing cell division are visible as darkened structures inside the Nuclear Membrane.

4. The DNA in Eukaryotic cells wraps tightly around Proteins called HISTONES. They help to maintain the shape of Chromosomes and aid in the tight packing of DNA.

5. Proteins called NONHISTONE Proteins Do Not participate in packing of DNA, they are involved in Controlling the Activity of Specific Regions of the DNA.

6. When preparing for Cell Division, Chromosomes form Copies of themselves, Each half of the Chromosome is called a CHROMATID or SISTER CHROMATIDS. Chromatids form as the DNA makes copies of itself before cell division.  (Figure 8-2)

7. The constricted area of each Chromatid is called a CENTROMERE . The Centromere holds the Two Chromatids together until the separate during Cell Division.

8. Between Cell Division, DNA IS NOT so Tightly Coiled into Chromosomes.  The Less tightly coiled DNA-Protein complex is called CHROMATIN .

9. Chromosomes are simpler in prokaryotes.  The DNA of most Prokaryotes comprises only ONE Chromosome, which is attached to the inside of the Cell Membrane.

10. Prokaryotic Chromosomes consist of a circular DNA Molecule and associated Proteins.

CHROMOSOME NUMBERS

1. EACH HUMAN BODY CELL CONTAINS 46 CHROMOSOMES, (2n) OR TWO COMPLETE SETS.

2. ANY CELL THAT CONTAINS TWO COMPLETE SETS OF CHROMOSOMES IS CALLED A DIPLOID CELL. A Diploid Cell  is commonly abbreviated as 2n.

3.  THE NUMBER OF CHROMOSOMES IN A DIPLOID CELL IS CALLED THE DIPLOID NUMBER.  EVERY ORGANISM HAS A CHARACTERISTIC DIPLOID NUMBER (2n).

4.  EXAMPLES:  FRUIT FLIES – 8, LETTUCE – 14, GOLDFISH 94, AND HUMANS 46.

5.  A CELL WITH ONLY ONE COMPLETE SET OF CHROMOSOMES IS CALLED A HAPLOID CELL.
A Haploid Cell is abbreviated as 1n.

6.  GAMETES, EGGS AND SPERM CONTAIN ONLY ONE COMPLETE SET.  EACH HUMAN SPERM OR EGG (GAMETE) CONTAINS 23 CHROMOSOMES, THE HAPLOID NUMBER (1n) FOR ALL HUMANS.

7.  WHEN AN EGG AND A SPERM OF THE SAME TYPE OF ORGANISM JOIN TO PRODUCE A NEW INDIVIDUAL, THE PROCESS IS CALLED FERTILIZATION.

8.  THE SINGLE CELL THAT RESULTS FROM FERTILIZATION IS KNOWN AS A ZYGOTE. THE ZYGOTE CONTAINS TWO COMPLETE SETS OF CHROMOSOMES, ONE SET FROM EACH GAMETE, FORMING A DIPLOID CELL.  IN MOST MULTICELLULAR ORGANISMS, THE ZYGOTE IS THE FIRST CELL OF THE NEW INDIVIDUAL.

9.  The Chromosomes in the Zygote exist in PAIRS.  For every Chromosome that was in the egg, there is a matching Chromosome from the sperm.

10. Human and Animal Chromosomes are categorized as either SEX CHROMOSOMES or AUTOSOMES.

11. SEX CHROMOSOMES are Chromosomes that Determine the SEX of an Organism.

12. In Humans, Sex Chromosomes are either X or Y.  Females have TWO X Chromosomes and Males have an X and Y Chromosome.

13. All the Other Chromosomes in an Organism are called AUTOSOMES.

14. TWO of the 46 Human Chromosomes are Sex Chromosomes, while the reaming 44 are Autosomes.

15. MATCH SET OF AUTOSOMES IN A DIPLOID CELL ARE CALLED HOMOLOGOUS PAIRS.  BOTH CHROMOSOMES IN A HOMOLOGOUS PAIR CONTAIN INFORMATION THAT CODE THE SAME TRAIT (GENES).  Example Eye Color.

SECTION 8-2, CELL DIVISION

All cells are derived from preexisting cells.  Cell division is the process by which cells produce offspring cells.  Cell division differs in prokaryotes and eukaryotes.  In eukaryotes, cell division differs in different stages of an organisms life cycle.

OBJECTIVES:  Describe the events of binary fission.  Describe each phase of the cell cycle.  Summarize the phases of mitosis.  Compare cytokinesis in animal cells with cytokinesis in plant cells.

CELL DIVISION IN PROKARYOTES

1. BINARY FISSION is the Division of a Prokaryotic cell INTO TWO Offspring Cells.

2. Binary Fission consist of THREE General Stages: (Figure 8-4):

STAGE 1 – The Chromosome, which is attached to the Inside of the Cell Membrane, makes a COPY of Itself, Resulting in Two Identical Chromosomes Attached to the Inside of the Inner Cell Membrane.

STAGE 2 – The Cell continues to grow until it reaches approximately TWICE its Normal Size. Then a CELL WALL Begins forms between the Two Chromosomes.

STAGE 3 – The Cell SPLITS into TWO NEW CELLS.  Each New Cell contains on the Identical Chromosomes.

CELL DIVISION IN EUKARYOTES

1. The trillions of cells that make up your body came from just ONE ORIGINAL CALLED: A FERTILIZED EGG (Zygote).  The Cell Theory states “CELLS COME ONLY FROM THE REPRODUCTION OF EXISTING CELLS” Chapter 4.

2. Each time A Cell Reproduces, the NEW Cells that are formed contained all the ESSENTIAL CYTOPLASM, ORGANELLES, AND NUCLEIC ACIDS NEEDED TO SURVIVE AND FUNCTION.

3. A Cell typically goes through PHASES during its Life, performing life processes of GROWTH AND DEVELOPMENT before it divides into new cells.

4. THE PHASES OF LIFE OF A CELL ARE CALLED THE CELL CYCLE . THE CELL CYCLE CONSISTS OF THREE PHASES:
      A. INTERPHASE
        B. MITOSIS
        C. CYTOKINESIS.

5. The CELL CYCLE is the Repeating Events that make up the Life of a Cell. (Figure 8-5)

6. Cell Division is One Phase of the Cell Cycle.  Cell Division consists of MITOSIS AND CYTOKINESIS.

7. MITOSIS is a Series of PHASES in Cell Division during which the NUCLEUS of a Cell Divides into TWO NUCLEI WITH IDENTICAL GENETIC MATERIAL.  MITOSIS OCCURS ONLY IN EUKARYOTES.

INTERPHASE

1. INTERPHASE IS THE PORTION OF THE CELL CYCLE BETWEEN DIVISION.

2. Interphase is the LONGEST Phase in the Cell Cycle of a typical Cell.  Interphase used to be referred to as the “RESTING PHASE”.

3. During Interphase, calls carry on all their usual functions, such as respiration and enzyme production.  The Cell also GROWS and DEVELOPS into MATURE FUNCTIONING Cells while in Interphase.  It is the period of normal metabolic activity.

4. INTERPHASE CONSIST OF THREE PHASES:

A. G1 PHASE – PERIOD OF NORMAL METABOLIC CELLULAR ACTIVITIES: THE NUMBER OF ORGANELLES AND AMOUNT OF CYTOPLASM IN A CELL INCREASE. Offspring Cells Grow to Mature Size.

B. S PHASE – THE GENETIC MATERIAL (DNA) IS DUPLICATED (COPIED).  THE CHROMOSOMES OF THE CELL REPLICATE.

C. G2 PHASE – Structure directly involved with mitosis are formed.  The Cell makes the Organelles and substances it needs for Cell Division.  A time during which the Cell prepares to divide.

5. REPLICATION IS THE PROCESS OF COPYING GENETIC MATERIAL.

6. REPLICATION RESULTS IN TWO IDENTICAL COPIES OF A CHROMOSOME CALLED SISTER CHROMATIDS.

7. CHROMOSOMES MUST REPLICATE DURING INTERPHASE SO THERE WILL BE A COMPLETE COPY OF EACH CHROMOSOME IN EACH NEW CELL.

8. BECAUSE THE DNA CONTAINED IN CHROMOSOMES CONTROL GROWTH DEVELOPMENT, AND FUNCTION OF EVERY CELL, EACH NEW CELL MUST HAVE AN EXACT COPY OF THE ORIGINAL SET OF CHROMOSOMES.
CELL DIVISION

1. CELL DIVISION IS THE PROCESS BY WHICH ONE CELL PRODUCES TWO NEW IDENTICAL DAUGHTER CELLS.

2. CELL DIVISION INVOLVES TWO STEPS: CALLED MITOTIC CELL DIVISION.

A.  MITOSIS – FIRST STEP. A SERIES OF  PHASES IN CELL DIVISION DURING WHICH THE NUCLEUS OF A CELL DIVIDES INTO TWO NUCLEI WITH IDENTICAL GENETIC MATERIAL.

B. CYTOKINESIS – SECOND STEP. THE CYTOPLASM OF THE CELL DIVIDES INTO TWO NEW CELLS CALLED DAUGHTER CELLS.

3. DAUGHTER CELL NUCLEI ARE IDENTICAL TO THE PARENT CELL NUCLEUS IN EVERY WAY.  LIKE THEIR PARENT CELL, SOME DAUGHTER CELLS WILL PASS THROUGH THE CELL CYCLE OF GROWTH, DEVELOPMENT, AND CELL DIVISION.

4. MULTICELLULAR ORGANISMS GROW AS MORE CELLS REPEAT THE CYCLE OF CELL DIVISION AND GROWTH.

MITOSIS

1. Mitosis is the Division of the Nucleus, which occurs during Cell Division.

2. Biologist have named the Steps, or Phases, of Mitosis to help study the process.  The FOUR Phases of Mitosis are called PROPHASE, METAPHASE, ANAPHASE, AND TELOPHASE. (Figure 8-6)

3. THE ACTUALLY PROCESS OF MITOSIS IS CONTINUOUS.

4. MITOSIS IS THE PROCESS BY WHICH A NUCLEUS GIVES RISE TO TWO IDENTICAL NUCLEI.

5. INTERPHASE PRIOR TO MITOSIS, THE PERIOD OF NORMAL METABOLIC ACTIVITY. The Chromosomes REPLICATE and the CYTOPLASM Increases as he cell prepares to divide. Interphase includes G1, S, G2 Phases of the Cell Cycle.

FOUR PHASES OF MITOSIS

PHASE 1- PROPHASE  (Figure 8-6 (a))

1. Chromatin condenses into Chromosomes of TWO Sister Chromatids joined together by the CENTROMERE, and visible when viewed through a microscope.

2. THE NUCLEOLUS AND NUCLEAR MEMBRANE DISAPPEAR.

3. TWO Structures called CENTROSOMES appear next to the Disappearing Nucleus.  In Animal Cells, each Centrosome contains a pair of small, cylindrical bodies called CENTRIOLES. Plant Cells lack Centrioles.

4. In BOTH Animal and Plant Cells, the Centrosomes move toward opposite poles of the cell. As they Separate, SPINDLE FIBERS made of microtubules radiate from the Centrosomes in preparation for Mitosis.  The array of Spindle fibers is called the MITOTIC SPINDLE, which serves to Equally divides the Sister Chromatids between the Two Offspring Cells.

5. There are TWO Type of Spindle Fibers:

A. KINETOCHORE FIBERS – They Attached to the Centromere Region of each Sister Chromatids.

B. POLAR FIBERS – they extend across the dividing cell from Centrosome to Centrosome.

PHASE 2 – METAPHASE  (Figure 8-6 (b))

1. The Chromosomes are moved to the CENTER of the CELL (Equatorial Plane) by the Kinetochore Fibers attached to the Centromeres.

2. The Two Sister Chromatids of each Chromosome are attached to Kinetochore Fibers radiating from OPPOSITE ENDS OF THE CELL.

PHASE 3 – ANAPHASE  (Figure 8-6 (c))

1. The Centromeres of Each Chromosome are pulled by the Kinetochore Fibers toward the ends of the cell (OPPOSITE POLES).

2. THE SISTER CHROMATIDS ARE THUS SEPARATED FROM EACH OTHER.  They are now Considered to be Individual Chromosomes.

PHASE 4 – TELOPHASE (Figure 8-6 (d))

1. After the Chromosomes reach opposite ends of the Cell, the Spindle Fibers Disassemble.

2. The Chromosomes return to less tightly coiled Chromatin State.

3. New Nuclear Envelope begins to form around the Chromosomes at each end of the cell.

4. CYTOKINESIS BEGINS.

5.  THE PROCESS OF MITOSIS IS NOW COMPLETE.  THE CELL MEMBRANE BEGINS TO PINCH THE CELL IN TWO AS CYTOKINESIS BEGINS.

CYTOKINESIS

1. Following the last phase of Mitosis, Cytokinesis COMPLETES the process of Cell Division.

2. During Cytokinesis, the Cytoplasm of a cell and its ORGANELLES SEPARATE INTO TWO NEW DAUGHTER CELLS.

3. Cytokinesis proceeds differently in animal and plant cells.

4. CYTOKINESIS OF ANIMAL CELLS: The Cytoplasm Divides when a GROOVE called the CLEAVAGE FURROW forms through the Middle of the Parent Cell.  The Cleavage Furrow Deepens until the parent cell pinches into TWO New Identical Cells.  The New Cells are Now in INTERPHASE.

5. CYTOKINESIS OF PLANT CELLS: In a Plant Cell, the material for NEW CELL WALL CALLED THE CELL PLATE  AND MEMBRANES GATHER AND FUSE ALONG THE EQUATOR, OR MIDDLE OF THE CELL, BETWEEN TWO NUCLEI. Forming TWO New Identical Cells.

6. In Both Animal and Plant Cells, New Offspring Cells are approximately equal in Size.

SECTION 8-3, MEIOSIS

Meiosis is a process of nuclear division that Reduces the number of chromosomes in new cells to Half the number in the original cell. The Halving of the chromosome number counteracts a fusion of cells later in the life cycle of the organism.  For example, in humans, meiosis produces haploid reproductive cells called GAMETES. Human gametes are sperm and egg cells, each which contains 23(1n) chromosomes.  The fusion of sperm and egg results in a zygote that contains 46 (2n) chromosomes.

OBJECTIVES:  List and describe the phases of meiosis.  Compare the end products of mitosis with those of meiosis.  Explain crossing-over and how it contributes to the production of unique individuals.  Summarize the major characteristics of spermatogenesis and oogenesis.

1. Most organisms are capable of COMBINING CHROMOSOMES FROM TWO PARENTS TO PRODUCE OFFSPRING.

2. WHEN CHROMOSOMES OF TWO PARENTS COMBINE TO PRODUCE OFFSPRING, THE PROCESS IS KNOWN As SEXUAL REPRODUCTION.

3. THE CHROMOSOMES THAT COMBINE DURING SEXUAL REPRODUCTION ARE CONTAINED IN SPECIAL REPRODUCTIVE CELLS CALLED GAMETES.

4. IN MOST ORGANISMS, GAMETES CAN BE EITHER EGG OR SPERM .

5. EGGS are larger than sperm and contain a lot of Cytoplasm.  An egg is nonmotile.

6. SPERM Cells contain very little Cytoplasm, have Flagella, that helps them swim to the nonmotile egg.

7. The Chromosomes of Two Gametes are added together when they join.  The number of Chromosomes in the offspring DOES NOT DOUBLE WITH EACH GENERATION, BUT REMAINS THE SAME BECAUSE OF MEIOSIS.

8. MEIOSIS IS THE WAY MANY ORGANISMS PRODUCE GAMETES THROUGH A TYPE OF CELL REPRODUCTION.

9. MEIOSIS IS A TYPE OF NUCLEAR DIVISION IN WHICH THE CHROMOSOME NUMBER IS HALVED.  LIKE MITOSIS, MEIOSIS IS FOLLOWED BY CYTOKINESIS.

10. IN HUMANS SPECIALIZED REPRODUCTIVE CELLS WITH 46 CHROMOSOMES (2n) (DIPLOID CELL) UNDERGO MEIOSIS AND CYTOKINESIS TO GIVE RISE TO EGG OR SPERM THAT HAVE ONLY 23 CHROMOSOMES (1N) (HAPLOID CELL) EACH.

11. MEIOSIS ONLY OCCURS IN EUKARYOTIC CELLS IN PHASES SIMILAR TO THE PHASES OF MITOSIS.

12. MEIOSIS IS DIFFERENT FROM MITOSIS IN SOME VERY IMPORTANT WAYS.

A. The process of meiosis results in the production of Daughter Cells that have HALF THE NUMBER OF CHROMOSOMES OF THE PARENT CELL (HAPLOID CELL).

B. Daughter Cell produced by meiosis ARE NOT ALL ALIKE.  THE DAUGHTER CELLS     MAY HAVE DIFFERENT CHROMOSOMES FROM EACH OTHER.

C. The NUMBER OF CELLS PRODUCED BY MEIOSIS IS DIFFERENT.

(1) Mitosis – One Parent Cell PRODUCES TWO DIPLOID DAUGHTER CELLS.

(2) Meiosis – One Parent Cell PRODUCES FOUR HAPLOID DAUGHTER CELLS.

STAGES OF MEIOSIS

1. THE PROCESS OF MEIOSIS SEPARATES THE PAIRS OF CHROMOSOMES IN A DIPLOID CELL TO FORM HAPLOID CELLS.

2. ONE PARENT CELL DIVIDES TWICE TO PRODUCE FOUR HAPLOID DAUGHTER CELLS.

3. DURING MEIOSIS, THE NUMBER OF CHROMOSOMES IN EACH CELL IS REDUCED FROM DIPLOID TO HAPLOID BY SEPARATING HOMOLOGOUS PAIRS OF CHROMOSOMES.

4. MEIOSIS PROCEEDS IN TWO MAIN STAGES:

A. MEIOSIS I HOMOLOGOUS PAIRS ARE SEPARATED.

B. MEIOSIS II THE SISTER CHROMATIDS OF EACH CHROMOSOME ARE SEPARATED.

MEIOSIS I  (Figure 8-9)

1. AT THE START OF MEIOSIS I EACH CHROMOSOME CONSIST OF TWO STRANDS OF SISTER CHROMATIDS CONNECTED AT THE CENTROMERE.

2. HOMOLOGOUS PAIRS OF CHROMOSOMES COME TOGETHER BEFORE MEIOSIS BEGINS, AN EVENT THAT DOES NOT OCCUR IN MITOSIS. THIS EVENT IS CALLED SYNAPSIS .

3. Each Pair of Homologous Chromosomes is called a TETRAD .

PROPHASE I.

1. Chromosomes become thick and visible, the chromosomes of each homologous pair are tangled together.

2. Portions of Chromatids may Break Off and attach to Adjacent Chromatids on the homologous Chromosome – a process called CROSSING-OVER. (Figure 8-10)

3. Crossing-Over results in Genetic Recombination by producing a New Mixture of Genetic Material.

4. Each pair consists of FOUR CHROMATIDS, BECAUSE EACH CHROMOSOME IN THE PAIR HAD REPLICATED BEFORE MEIOSIS BEGAN.

5. The Nucleoli and the Nuclear Envelope disappear and the spindle fibers form.

METAPHASE I.  Homologous pairs (Tetrads) are still together and arrange in the middle of the cell.

ANAPHASE I.  The homologous pairs of chromosomes separate from each other, spindle fibers pull one member from each pair to opposite ends of the cell. The Random separation of the Homologous Chromosomes is called INDEPENDENT ASSORTMENT.

TELOPHASE I.  Cytokinesis takes place; each new cell is haploid, containing one chromosome
from each pair.

MEIOSIS II  (Figure 8-11)

1. CHROMOSOMES DO NOT REPLICATE BEFORE BEGINNING THE SECOND PHASE MEIOSIS II WILL DIVIDE CHROMOSOMES INTO HAPLOID CELLS CALLED GAMETES.

2. Each Diploid Cell from Meiosis I will go through a second division, forming the FOUR GAMETES HAPLOID CELL.  (Review Figure 8-11)

CROSSING-OVER

1. CHROMOSOMES OF ALL ORGANISMS CONTAIN REGIONS CALLED GENES .

2. EACH GENE CODES FOR ONE TRAIT, OR CHARACTERISTIC, OF THE ORGANISM.

3. ONE VERY IMPORTANT EVENT THAT CAN OCCUR DURING MEIOSIS I IS CROSSING- OVER.

4. CROSSING-OVER IS THE EXCHANGE OF GENES BETWEEN PAIR OF HOMOLOGOUS CHROMOSOMES.

5. CROSSING-OVER OCCURS ONLY DURING PROPHASE I (ONLY!) WHEN HOMOLOGOUS PAIRS ARE STILL JOINED TOGETHER.  THESE PAIRS CAN SOMETIMES BREAK WHERE THEY MEET AN EXCHANGE GENES. (Figure 8-10)

FORMATION OF GAMETES

1. In Animals, meiosis produces haploid reproductive cells called GAMETES.

2. Meiosis occurs within the Reproductive Organs, in the TESTES or OVARIES.

3. In the Testes, meiosis is involved in the production of Male Gametes known as Sperm Cells or Spermatozoa.

4. In the development of Sperm Cells, a Diploid Reproductive Cell divides Meiotically to form FOUR Haploid Cells called SPERMATIDS.

5. Each Spermatid then develops into a Mature Sperm Cell.

6. The production of Sperm Cells is called SPERMATOGENESIS . (Figure 8-12 (b))
7. OOGENESIS is the production of Mature Egg Cells or OVA.  (Figure 8-12 (c))

8. Notice that the Female only produces ONE EGG (OVUM) under normal circumstances.

9. Although creating 4 Haploid Cells through meiosis, only One Becomes the Egg, the other Three products of meiosis are called POLAR BODIES ,and Degenerate.  This is due to the unequal dividing of the cytoplasm during Cytokinesis I & II.

ASEXUAL AND SEXUAL REPRODUCTION

1. EVOLUTION IS THE PROCESS OF CHANGE IN LIVING POPULATIONS OVER TIME.

2. ASEXUAL REPRODUCTION is the production of Offspring from ONE PARENT.

3. Asexual reproduction DOES NOT Usually involve Meiosis or the Union of Gametes.

4. In Unicellular Organisms, such as bacteria, New Organisms are created by either BINARY FISSION or MITOSIS.

5. Asexual Reproduction in multicellular organisms results from BUDDING OFF a Portion of Their Bodies. (Plants)

6. The Offspring From Asexual Reproduction are Genetically Identical to the Parent.

7. SEXUAL REPRODUCTION is the Production of Offspring through Meiosis and the Union of a Sperm and an Egg.

8. MEIOSIS AND SEXUAL REPRODUCTION RESULTS IN NEW COMBINATIONS OF CHARACTERISTICS WITHIN A POPULATION.

9. ORGANISMS IN A POPULATION THAT REPRODUCE SEXUALLY ARE NOT ALL ALIKE.

10. DIFFERENCES AMONG MEMBERS OF A POPULATION ARE COLLECTIVELY CALLED VARIATION.  WHICH RESULTS FROM THE RECOMBINATION OF GENES DURING MEIOSIS AND FERTILIZATION.

11. MEIOSIS AND FERTILIZATION SHUFFLE THE GENES FROM PARENT ORGANISMS, PRODUCING NEW COMBINATIONS OF GENES IN THE OFFSPRING.

12. AN ORGANISMS CHARACTERISTICS ENABLE IT TO SURVIVE IN IT’S ENVIRONMENT. THE CONDITIONS OF THE ENVIRONMENT DETERMINE WHICH CHARACTERISTICS OR TRAITS BENEFIT THE SURVIVAL AND WHICH DO NOT.

13. THE ORGANISMS WITH THE TRAITS TO SURVIVE WILL THEN REPRODUCE TO PASS THOSE POSITIVE TRAITS ON TO THEIR OFFSPRING.

14. OVER TIME THIS PROCESS LEADS TO THE CHANGE IN THE POPULATIONS, BECAUSE ONLY THOSE WITH POSITIVE TRAITS TO PASS ON WILL REPRODUCE.  NATURAL SELECTION.

15. THE ACCUMULATION OF SUCH GENES AND TRAITS IN EACH GENERATION IS THE BASIS OF EVOLUTION.

16. SINCE ASEXUAL OFFSPRING HAVE THE EXACT SAME GENES AND TRAITS AS THE PARENT, GENETIC VARIATION RARELY OCCURS.

17. A CHANGE IN THE ENVIRONMENT THAT CAN DESTROY ONE INDIVIDUAL COULD DESTROY THE ENTIRE POPULATION.

Protein Synthesis Quiz

Name: 

DNA & Protein Synthesis

 

 

True/False
Indicate whether the sentence or statement is true or false.
1.
When a tRNA anticodon binds to an mRNA codon, the amino acid detaches from the tRNA molecule and attaches to the end of a growing protein chain.
2.
Only ribosomal RNA plays a role in translation.
3.
During DNA replication, the molecule unzips and the exposed DNA nucleotides pair with other
specific nucleotides present in the nucleus
4.
Humans pass exact copies of their DNA to their offspring.
5.
Watson and Crick proposed a model of DNA
6.
Amino acids are linked together by hydrogen bonds.
7.
During transcription, the information on a DNA molecule is “rewritten” into an mRNA molecule.
8.
All codons encode amino acids.
 

Multiple Choice
Identify the letter of the choice that best completes the statement or answers the question.
9.
Purines and pyrimidines are
a.
bases found in amino acids.
b.
able to replace phosphate groups from defective DNA.
c.
names of specific types of DNA molecules.
d.
bases found in nucleotides.
10.
Chargaff’s rules, or the base-pairing rules, state that in DNA
a.
the amount of adenine equals the amount of thymine.
b.
the amount of guanine equals the amount of cytosine.
c.
the amount of guanine equals the amount of thymine.
d.
Both a and b
11.
ATTG : TAAC ::
a.
AAAT : TTTG
c.
GTCC : CAGG
b.
TCGG : AGAT
d.
CGAA : TGCG
12.
Which of the following types of RNA carries instructions for making proteins?
a.
mRNA
c.
tRNA
b.
rRNA
d.
All of the above
13.
Which of the following is not found in DNA?
a.
adenine
c.
uracil
b.
cytosine
d.
None of the above
14.
Suppose that you are given a polypeptide sequence containing the following sequence of amino acids: tyrosine, proline, aspartic acid, isoleucine, and cysteine. Use the portion of the genetic code given in the table below to determine the DNA sequence that codes for this polypeptide sequence.
mRNA
Amino acid
UAU, UAC
tyrosine
CCU, CCC, CCA, CCG
proline
GAU, GAC
aspartic acis
AUU, AUC, AUA
isoleucine
UGU, UGC
cysteine
a.
AUGGGUCUAUAUACG
c.
GCAAACTCGCGCGTA
b.
ATGGGTCTATATACG
d.
ATAGGGCTTTAAACA
15.
In order for protein synthesis to occur, mRNA must migrate to the
a.
ribosomes.
c.
RNA polymerase.
b.
lac operon.
d.
heterochromatin.
16.
After the primary structure of a protein has been completed
a.
the codons and anticodons unite.
b.
an enzyme attaches adjacent amino acids to each other to form a chain.
c.
the protein folds into the secondary and tertiary structures.
d.
the tRNA molecules remain attached until the protein is secreted from the cell.
17.
Which of the following is  not  part of a molecule of DNA?
a.
deoxyribose
c.
phosphate
b.
nitrogenous base
d.
ribose
18.
During replication in a molecule of DNA, one separation likely to occur is between
a.
cytosine and guanine
c.
ribose and adenine
b.
phosphate and deoxyribose
d.
uracil and thymine
19.
A gene may be described as
a.
a sequence of amino acids.
b.
special proteins found in chromosomes.
c.
a sequence of nucleotides that controls the production of a certain protein.
d.
a sequence of nucleotides coding for the production of starches and sugars.
20.
The synthesis of a new double strand of DNA begins when the two strand of the original DNA helix
a.
‘unzip’.
c.
attract nitrogenous bases.
b.
act as a template.
d.
destroy a genetic code.
21.
Genes(DNA) affect cell structure and function by directing the synthesis of
a.
nucleic acids
c.
nucleotides
b.
hereditary traits
d.
proteins
22.
Protein molecules are made up of
a.
fats
c.
lipids
b.
nucleotides
d.
amino acids
23.
During, DNA replication, DNA
a.
converts to RNA
c.
joins mRNA
b.
joins tRNA
d.
strands separate
24.
Which is not true about proteins?
a.
They control biochemical pathways within the cell.
b.
They direct the synthesis of lipids.
c.
They are composed of sugars.
d.
They take responsibility for cell movement.
25.
Molecules of DNA are composed of long chains of
a.
amino acids.
c.
monosaccharides.
b.
fatty acids.
d.
nucleotides.
26.
Watson and Crick were the first scientists to state that DNA
a.
contains phosphate groups
c.
has four nitrogen bases
b.
undergoes transcription
d.
has a double helix shape
27.
The two chains of a DNA molecule are connected by
a.
nitrogen bonds
c.
bases
b.
relatively weak chemical bonds
d.
nucleotides
28.
All nucleotide molecules contain the same kind of
a.
ribose sugar
c.
pyrimidine
b.
purine
d.
phosphate group
29.
After DNA replication, the two DNA molecules that are made
a.
are complementary.
c.
must replicate again.
b.
are identical.
d.
cannot replicate again.
30.
Sixty-four codons for 20 amino acids requires that
a.
some amino acids lack codons
b.
some amino acids have more than one codon
c.
all amino acids have two codons
d.
none of the above
31.
Which of the following combines with amino acids
a.
DNA
c.
tRNA
b.
mRNA
d.
B and C
32.
rRNA has a function in
a.
synthesizing DNA.
c.
forming ribosomes.
b.
synthesizing mRNA.
d.
transferring amino acids to ribosomes.
33.
The DNA code consists of sequences of nucleotides arranged in groups of
a.
variable number
c.
threes
b.
twos
d.
fours
34.
Unlike mRNA, the DNA molecule is
a.
double-stranded
c.
like a ladder
b.
single-stranded
d.
both A and C
35.
The number of bases in a row in a gene that codes a protein composed of 200 amino acids is
a.
200
c.
600
b.
400
d.
800
36.
A DNA molecule unzips during
a.
replication
c.
translation
b.
transcription
d.
both A and B
37.
A DNA chain has the following sequence of bases, TAG.  The corresponding messenger RNA
chain should have the sequence
a.
ATC
c.
ATG
b.
UTC
d.
AUC
38.
Unlike DNA, RNA
a.
contains deoxyribose.
c.
contains thymine.
b.
is double stranded.
d.
contains uracil.
39.
Which molecule contains deoxyribose
a.
DNA
c.
tRNA
b.
mRNA
d.
both B and C
40.
Each combination of three nitrogenous bases on messenger RNA forms a (an)
a.
anticodon.
c.
enzyme.
b.
codon.
d.
nuclei acid.
41.
In RNA, uracil is complementary to:
a.
guanine
c.
thymine
b.
adenine
d.
cytosine
42.
Once a molecule of transfer RNA has released its amino acid, the tRNA
a.
becomes attached to messenger RNA.
b.
becomes attached to ribosomal RNA.
c.
is destroyed as an individual molecule.
d.
moves away to pick up another amino acid.
43.
If the sequence of bases in a segment of a DNA strand were cytosine, guanine, adenine, thymine, adenine, then the sequence in a complimentary strand of newly-made mRNA would be
a.
cytosine, uracil, adenine, guanine, uracil
c.
uracil, adenine, cytosine, uracil, guanine
b.
guanine, cytosine, uracil, adenine, uracil
d.
cytosine, guanine, uracil, uracil, adenine
44.
Which sugar is present in RNA
a.
glucose
c.
ribose
b.
sucrose
d.
deoxyribose
45.
RNA differs from DNA, in that RNA
a.
is single-stranded.
c.
contains the nitrogen base uracil.
b.
contains a different sugar molecule.
d.
All of the above are correct.

 

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