Category: Curriculum Map

Cell Size

THE SURFACE AREA TO VOLUME RATIO OF A CELL
INTRODUCTION:

Cells are limited in how large they can be. This is because the surface area and volume ratio does not stay the same as their size increases. Because of this, it is harder for a large cell to pass materials in and out of the membrane, and to move materials through the cell.
In this lab, you will make cube shaped models to represent cells. The dimension along one side will be doubled with each model. You will then calculate the surface area, volume, and the ratio between the two.

MATERIALS:

Scissors
Construction paper
Tape
Metric ruler

PROCEDURE:

1. Construct three cell models like the pattern shown. The dimensions of a side will double each time, with the sides being 2 cm, 4 cm, and 8 cm. Fold and tape into cubes with the tabs to the inside. Record the dimensions in the DATA TABLE (the first one is done for you in the table).

 

 

DATA TABLE: Cell Size Comparison
Cell Dimensions
(cm)		 Surface Area
(cm2)		 Volume
(cm3)		 Ratio
Surface area to Volume
1 2 X 2 X 2
2
3

 

CALCULATIONS:

2. Calculate the total surface area for each cell model by the following formula:

surface area = (Length X Width) X 6 sides

 

Record the surface areas in the DATA TABLE.

3. Calculate the volumes for each cell model by the following formula:

volume = length X width X height

 

Record the volumes in the DATA TABLE.

4. Calculate the surface area-to-volume ratio for each cell model by the following formula:

ratio = surface area
volume

 

Record the ratio values in the DATA TABLE.
These ratios show how many times larger the surface area is as compared to the volume. Notice that it becomes less than one very quickly.

QUESTIONS:

1. Which model has the largest surface area?

2. Which model has the largest volume?

3. Which model has the largest ratio?

4. To maintain life, and carry-out cellular functions, materials must be able to move into and out of the cell. Also, material needs to be able to move within the cell. What might be the advantage of having a large surface area?

 

 

5. What might be the disadvantage of having a large volume?

 

Chapter 10 Questions PPT

DNA & Replication
ppt Questions

History of DNA

1. Early scientists believed that _________ was the genetic material of the cell. Explain why.

 

2. Proteins are made of 20 different ________________.

3. Long chains of amino acids make up _________________.

4. Fred Griffith worked with what type of bacteria?

5. What did he find to be true after his experiments with the S and R strains of bacteria?

 

6. This process of picking up DNA from the environment is called ____________________.

7. Did Griffith’s experiment prove DNA was the genetic material?

8. What 2 main things make up chromosomes?

9. What did Hershey and Chase use in their experiments to prove DNA was the cell’s genetic material?

10. Hershey and Chase radioactively tagged the viral DNA with _______________ and the protein capsid with ______________.

11. Which radioactive substance was injected into and took over the host cell’s DNA?

12. What scientists showed the amount of the 4 nitrogen bases present in DNA?

13. Name the bases and their amounts found in somatic or body cells of humans.

 

14. What bases are complementary (pair with each other) on DNA?

15. What type of bonds join base pairs on DNA?

16. Are these strong or weak bonds?

17. What was Rosalind Franklin’s contribution to finding DNA’s structure?

 

18. Who built the first model of DNA and what did they use to help get the correct measurements for the molecule?

DNA Structure 

19. DNA is two coiled strands known as a ___________________.

20. What makes up the sides of a DNA molecule?

21. What bonds the deoxyribose sugars to the phosphate groups?

22. Where are the nitrogen bases found on DNA & how are they bonded?

23. Most DNA has a ______________ twist with ________ base pairs in a complete turn.

24. DNA stands for ___________________________.

25. ______________ are the subunits making up DNA.

26. Name the 3 parts of a nucleotide.

 

27. Why is deoxyribose called a pentose sugar?

28. Sketch a pentose sugar and include the carbons correctly numbered.

 

 

29. The sugars on one DNA strand of DNA are from 5′ to _________, while the sugars on the other strand are antiparallel running from _________ to ___________.

30. Double ring nitrogen bases are called ____________, while single ring nitrogen bases are called ________________.

31. Name the purines.

32. Name the pyrimidines.

33. Purines will only pair with ________________.

34. __________ hydrogen bonds are required to join guanine to cytosine, while only ________ bonds join adenine to thymine.

35. If there is 30% adenine, how much cytosine is present?

DNA Replication 

36. Cells must copy their DNA before they do what? Explain why.

 

37. During what part of the cell cycle is DNA copied?

38. In eukaryotes, where are the copies of DNA made?

39. The process of copying DNA is known as DNA ________________.

40. Replication of DNA begins at points called __________________________.

41. The two DNA strands ______________ at origins of replication forming Y-shaped areas called _______________________.

42. New DNA strands grow at the _____________.

43. As the two strands open at the origins of replication, replication _____________ form.

44. Prokaryotic chromosomes have a ___________ replication bubble, while eukaryotic cells have ___________ bubbles.

45. What enzyme uncoils DNA so it can be replicated or copied?

46. What other job does this enzyme perform?

47. What is the job of single-strand binding proteins?

 

48. What enzyme relieves stress on the DNA strands at the replication forks?

49. An RNA __________ must be present to start the addition of new nucleotides.

50. What enzyme makes the RNA primer?

51. DNA ______________ is the enzyme that adds new complementary base pairs.

52. DNA polymerase can only add nucleotides to what end of the DNA molecule?

53. Which DNA strand is synthesized continuously as a single strand?

54. The leading strand is made from the ______________ toward the _________________ as it opens.

55. The ____________ strand is synthesized ____________ the overall direction of replication.

56. The lagging strand is made in __________ short segments from the _____________ fork toward the _____________ of replication.

57. The short segments of the lagging strand are called ______________ fragments.

58. What enzyme joins Okazaki fragments together into one strand?

59. Sketch half of a replication bubble. Label the point of origin and the replication fork. Draw two arrows showing the leading strand and the lagging strand.

 

 

 

60. DNA polymerase initially makes how many errors?

61. Proofreading enzymes correct mistakes in DNA to a one in ______________ base pairing errors?

62. Explain the semiconservative model for DNA replication.

 

 

63. Who developed this idea about replication?

64. Chemicals and _____________ radiation can damage DNA in our body cells so it must be ________________ repaired.

65. What is excision repair?

 

66. What 2 enzymes replace damaged sections of DNA and rebind the molecule?

67. What is the complementary DNA strand for: 5′ -CGTATG -3′ ?

 

 

Cell Structure and Function Assi

 

Cell Structure and Function Assignment

 

Project Due – Tuesday, June 28

Instructions:

  1. Draw and color EACH CELL or CELL ORGANELLE on a separate sheet of unlined paper. BE SURE TO CENTER YOUR DRAWING ON THE PAGE!
  2. At the top of each page, CENTER and WRITE THE NAME of the cell or organelle that you are drawing.
  3. LABEL all parts of the cell and/or organelle.
  4. WRITE the function of EACH LABELED PART (FUNCTION MUST BE WRITTEN NEXT TO THE LABEL).
DRAWINGS:

  1. Prokaryote diagram
  2. Animal Cell Diagram
  3. Plant Cell Diagram
  4. Nucleus & Nucleolus Diagram
  5. Rough and Smooth Endoplasmic Reticulum Diagram
  6. Golgi Diagram
  7. Chloroplast Diagram
  8. Mitochondria Diagram
  9. Lysosome Diagram
  10. Cell Membrane Diagram

Chapter 13 Biotechnology PPT Questions

 

DNA Technology
ppt Questions

DNA Extraction

1. When cells are treated with certain chemicals, it causes the plasma membrane to __________ or lyse.

2. DNA can be pulled out of cells because it is ________________ and can be ______________.

3. Describe the appearance of DNA spooled from cells.

 

4. What may be used to cut DNA into smaller pieces?

5. Do all restriction enzymes cut DNA at the same place?

6. What 2 properties can be used to separate DNA fragments?

7. Why does DNA have a negative charge?

 

8. To separate DNA fragments, it is placed in a ____________ with a current of _____________ running through it.

9. This process is called ____________________.

10. What determines the direction DNA will move in a gel?

 

11. Which fragments move further and faster?

12. DNA fragments are loaded into depression on the gel called _____________.

13. The DNA gel floats in a chamber covered with a ____________ solution.

14. DNA fragments closest to the wells are ___________ in size, while the __________ DNA fragments are further from the wells.

Steps in DNA Sequencing 

15. Many copies of a ______________ of DNA are placed in a test tube and ________________ is added to begin the process.

16. What else must be added?

17. How are the different nucleotide bases marked or tagged?

 

18. Dyed and _____________ nucleotides are added, but the large __________ molecules stop the chain from growing producing DNA fragments of _______________ sizes.

19. The fragments make banding patterns on an electrophoresis gel of different _____________ that can be identified.

20. The separated fragments are then read by _____________ from the ________ of the gel to the top.

Copying DNA

21. Define PCR and tell what it stands for.

 

 

22. To make many copies of DNA, DNA polymerase is added that can work at very high _______________. 

23. DNA is _____________ to separate the two strands.

24. What is added next to the test tube of DNA and DNA polymerase?

25. What are primers?

 

26. When the tube is cooled, DNA polymerase adds new ___________ to the separated DNA strands.

27. Even though a small amount of DNA is used to start PCR, ___________ amounts of DNA can be copied.

Cloning

28. What is a clone?

 

29. Clones may be produced by _____________ reproduction.

30. What two types of cells are combined in order to clone an organism?

 

31. Once a body cell fuses to an egg cell, the cell divides like a normal _____________.

32. What was the first successfully cloned organism?

 

Human Genome Project

33.  When was the project started?

34. What is the goal of the project?

 

 

35. How many nucleotides approximately make up the human genome?  How many chromosomes?

 

36. Who is mapping the genes on the human chromosomes?

37. From working on the Human Genome project, scientists have discovered that only about ________ actually codes for proteins; these genes are called _____________.

38. What is the other 98% or non-coding genes of DNA known as?

39. How many genes have been found on DNA?  Is this more or less than the expected number?

 

40. What are SNP’s that the scientists found?

 

41. Define proteome.

 

42. Human Genome researchers discovered transposons.  What are these structures?

 

43. The Human Genome Project was produced an area of science known as bioinformatics.  how is this helpful in sequencing DNA?

 

44. Define biotechnology.

 

45. Give an example of an agricultural crop grown in this area that has been improved by genetic engineering.

46. What product was 1st made in 1982 by genetic engineering to help diabetics?

47. Explain how biotechnology has improved each of these fruits or vegetables:

a. bananas

b. rice

c. garlic

d. potatoes

 

48. Give 4 ways biotechnology has helped the environment.

 

 

Cell Worksheet Ch4 BI

 

 

Cells

 

Section 4-1 Introduction to the Cell

1. What is a cell?

2. Who was the first person to use a simple microscope and view microscopic organisms?

3.. What English scientist was first to view dead plant cells?

4. State the 3 parts to the cell theory.

5. Tell how each of these scientists contributed to the cell theory — Matthias Schleiden, Theodor Schwann, and Rudolf Virchow.

6. Give 3 ways that cells are not alike.

7. What is one of the longest animal cells?

8. Explain why cells are limited in how large they can grow.

9. The shape of a cell reflects its ______________________. Give an example of this.

10. Define organelle & tell what they do for a cell.

11. What surrounds the outside of all cells?

12. Where is the nucleus of a cell & what does it do?

13. What two characteristics do all eukaryotes share?

14. What type of cell is a bacterium?

15. Where is the genetic information (chromosome) of a bacterium found?

16. What are prokaryotes & are they in the same kingdom as eukaryotes?

Section 4-2 Parts of the Eukaryotic Cell

17. Why can cells not survive if they are totally isolated from their environment?

18. What controls what enters or leaves a cell?

19. Define selectively permeable.

20. Describe the phospholipid make up of cell membranes.

21. Cells are bathed in an aqueous environment. What does this mean?

22. Sketch the lipid bilayer of a cell showing the inside & outside of the cell. Be sure to label all parts of the membrane.

23. What is the difference between peripheral & integral proteins in the cell membrane?

24. What is the purpose of the carbohydrate tails attached to some integral proteins?

25. Do all integral proteins look alike? Explain.

26. Explain the fluid mosaic model of the cell membrane.

27. Name 12 organelles found in cells. (See table 4-2)

28. In what part of a cell are organelles found?

29. What is cytosol & what does it contain?

30. Name 3 organelles found in plant, but not animal cells. (See bottom of table 4-2)

31. What is the function of mitochondria? What energy molecule is made there?

32. Why do liver & muscle cells have more mitochondria than other types of cells?

33. Describe the outer covering of the mitochondria.

34. What are cristae & what is their purpose?

35. Mitochondria are able to reproduce inside cells because they have their own ___________.

36. What organelles are the most numerous inside cells?

37. What two things make up ribosomes & are ribosomes surrounded by membrane like other organelles?

38. Ribosomes are made inside the _______________ of a cell.

39. Ribosomes may remain unattached or __________ in cytosol or attach to what other organelle’s surface?

40. What organic compounds to ribosomes synthesize or make?

41. What does ER stand for & what is the ER in a cell?

42. What is the ER’s function?

43. Name the two types of ER inside cells.

44. What is on the surface of rough ER?

45. Rough ER synthesizes large amounts of _________________ for cells.

46. Give 3 functions of smooth ER.

47. What is the Golgi apparatus?

48. Golgi is a system of ________________ or flattened _____________.

49. How does the Golgi work with the endoplasmic reticulum of a cell?

50. What are lysosomes & what do they do?

51. Name 8 things that the enzymes inside lysosomes digest?

52. In what type of cells are lysosomes common? In what type of cell are they rare?

53. Where is the cytoskeleton & what is its function?

54. What are the two major components of the cytoskeleton?

55. How do microfilaments & microtubules differ from each other?

56. What are spindle fibers & what are they made of?

57. What protein makes up microfilaments?

58. Compare cilia & flagella.

59. What is the purpose of the nuclear matrix?

60. What double membrane surrounds the nucleus?

61. Where is chromatin found & what 2 things is it made of?

62. When a cell is ready to divide, chromatin condenses & coils into _____________________.

63. What is the purpose of DNA inside the nucleus?

64. How do nuclear pores help RNA?

65. Where is the nucleolus found and what is made there?

66. Where is the cell wall in plants found, what is its function, and what is it made of?

67. What are the 2 types of cell walls in plants?

68. What is the difference between the primary & secondary cell walls?

69. What is found inside plant vacuoles?

70. What takes up much of the volume of plant cells? What happens to the other organelles?

71. How are plastids similar to mitochondria?

72. What is found inside of plastids?

73. Name the most familiar plastid & tell its function.

74. What are thylakoids?

Section 4-3 Multicellular Organization

75. Cells are organized into ______________________. Give an example.

76. What is an organ & give an example?

77. What forms organ systems?

78. The digestive system is an organ system. Name the organs that make up this system.

79. All the systems working together make up an ______________________ such as a plant or animal.

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